Code | CSB-EL004976HU |
Size | 96T,5×96T,10×96T |
Price | Request a Quote or Start an on-line Chat |
Trial Size |
24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now |
Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
Inter-assay Precision (Precision between assays): CV%<10% | |||||||
Three samples of known concentration were tested in twenty assays to assess. | |||||||
To assess the linearity of the assay, samples were spiked with high concentrations of human CDA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
Sample | Serum(n=4) | ||||||
1:1 | Average % | 84 | |||||
Range % | 80-92 | ||||||
1:2 | Average % | 97 | |||||
Range % | 91-105 | ||||||
1:4 | Average % | 102 | |||||
Range % | 98-110 | ||||||
1:8 | Average % | 93 | |||||
Range % | 86-98 |
The recovery of human CDA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
Sample Type | Average % Recovery | Range | |||||
Serum (n=5) | 95 | 89-100 | |||||
EDTA plasma (n=4) | 88 | 84-92 | |||||
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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This Human CDA ELISA Kit was designed for the quantitative measurement of Human CDA protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL.
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i curiously of human CDA ELISA kit about the last step
the protocol say using 450 nm to read plate but also saying can use 540 or 570 nm if availability and 450 nm may be higher and less accurate?? and what kind wavelength do i use?
450nm? 540 nm ? or 570 nm
or which wavelength is you recommended?