Target Name
ADAM metallopeptidase domain 2
Alternative Names
A disintegrin and metalloproteinase domain 2 ELISA Kit; ADAM 2 ELISA Kit; ADAM metallopeptidase domain 2 ELISA Kit; Adam2 ELISA Kit; ADAM2_HUMAN ELISA Kit; AI323749 ELISA Kit; Cancer/testis antigen 15 ELISA Kit; CRYN1 ELISA Kit; CRYN2 ELISA Kit; CT15 ELISA Kit; Disintegrin and metalloproteinase domain-containing protein 2 ELISA Kit; Fertilin beta ELISA Kit; Fertilin subunit beta ELISA Kit; Ftnb ELISA Kit; PH 30 ELISA Kit; PH 30b ELISA Kit; PH-30 ELISA Kit; PH30 ELISA Kit; Ph30 beta ELISA Kit; PH30-beta ELISA Kit
Species
Homo sapiens (Human)
Sample Types
serum, plasma, tissue homogenates
Detection Range
0.312 ng/ml - 20 ng/ml
Detection Wavelength
450 nm
Assay Principle
quantitative
Precision
Intra-assay Precision (Precision within an assay): CV%<8% | | | |
Three samples of known concentration were tested twenty times on one plate to assess. | |
Inter-assay Precision (Precision between assays): CV%<10% | | | |
Three samples of known concentration were tested in twenty assays to assess. | | |
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human ADAM2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
|
| Sample | Serum(n=4) | |
1:1 | Average % | 101 | |
Range % | 97-105 | |
1:2 | Average % | 106 | |
Range % | 102-111 | |
1:4 | Average % | 90 | |
Range % | 86-96 | |
1:8 | Average % | 97 | |
Range % | 90-104 | |
Recovery
The recovery of human ADAM2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
Sample Type | Average % Recovery | Range | |
Serum (n=5) | 102 | 95-109 | |
EDTA plasma (n=4) | 96 | 91-103 | |
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| | | | | | |
ng/ml | OD1 | OD2 | Average | Corrected | |
20 | 2.305 | 2.234 | 2.269 | 2.100 | |
10 | 1.989 | 1.967 | 1.978 | 1.809 | |
5 | 1.447 | 1.388 | 1.418 | 1.248 | |
2.5 | 0.788 | 0.779 | 0.783 | 0.614 | |
1.25 | 0.433 | 0.421 | 0.427 | 0.258 | |
0.625 | 0.330 | 0.323 | 0.327 | 0.157 | |
0.312 | 0.266 | 0.274 | 0.270 | 0.101 | |
0 | 0.173 | 0.165 | 0.169 | | |
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
