Human EGF-containing fibulin-like extracellular matrix protein 1(EFEMP1) ELISA kit

Code CSB-EL007450HU
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Description

This Human EFEMP1 ELISA Kit was designed for the quantitative measurement of Human EFEMP1 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 1.56 ng/mL-100 ng/mL and the sensitivity is 0.39 ng/mL.

Target Name EGF-containing fibulin-like extracellular matrix protein 1
Alternative Names DHRD ELISA Kit; Doyne honeycomb retinal degeneration ELISA Kit; DRAD ELISA Kit; EFEMP 1 ELISA Kit; EFEMP1 ELISA Kit; EGF containing fibulin like extracellular matrix protein 1 ELISA Kit; EGF containing fibulin like extracellular matrix protein 1 precursor ELISA Kit; EGF-containing fibulin-like extracellular matrix protein 1 ELISA Kit; Epidermal growth factor containing fibulin like extracellular matrix protein 1 ELISA Kit; Extracellular protein S1 5 ELISA Kit; Extracellular protein S1-5 ELISA Kit; FBLN 3 ELISA Kit; FBLN3 ELISA Kit; FBLN3_HUMAN ELISA Kit; FBNL ELISA Kit; FIBL 3 ELISA Kit; FIBL-3 ELISA Kit; FIBL3 ELISA Kit; Fibrillin like ELISA Kit; Fibrillin like protein ELISA Kit; Fibrillin-like protein ELISA Kit; FIBULIN 3 ELISA Kit; Fibulin-3 ELISA Kit; Fibulin3 ELISA Kit; FLJ35535 ELISA Kit; Heat shock 70 KD protein 1 ELISA Kit; MGC111353 ELISA Kit; MLVT ELISA Kit; MTLV ELISA Kit; S1 5 ELISA Kit; T16 protein ELISA Kit
Abbreviation EFEMP1
Uniprot No. Q12805
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 1.56 ng/mL-100 ng/mL
Sensitivity 0.39 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Neuroscience
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human EFEMP1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %102
Range %98-109
1:2Average %84
Range %80-88
1:4Average %90
Range %85-94
1:8Average %93
Range %89-97
Recovery
The recovery of human EFEMP1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 8983-94
EDTA plasma (n=4)9590-100
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
1002.261 2.127 2.194 2.074
501.539 1.500 1.520 1.400
250.854 0.902 0.878 0.758
12.50.517 0.545 0.531 0.411
6.250.315 0.304 0.310 0.190
3.120.226 0.233 0.230 0.110
1.560.170 0.182 0.176 0.056
00.121 0.118 0.120
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Background

Function
(From Uniprot)
Binds EGFR, the EGF receptor, inducing EGFR autophosphorylation and the activation of downstream signaling pathways. May play a role in cell adhesion and migration. May function as a negative regulator of chondrocyte differentiation. In the olfactory epithelium, it may regulate glial cell migration, differentiation and the ability of glial cells to support neuronal neurite outgrowth.
Gene References into Functions
  1. The extracellular matrix glycoprotein EFEMP1 is promising for future functional studies. PMID: 28374850
  2. The results demonstrate the implication of Fibulin-3 in malignant mesothelioma due to fluoro-edenite exposure. PMID: 29664355
  3. This study found a nominally different distribution of EFEMP1 genotypes, alleles and haplotypes between suicidal subjects and controls. PMID: 28327445
  4. Glioma risks were higher in carriers of single-nucleotide polymorphisms (SNPs) rs1346787 and rs3791679 in EGF-containing fibulin-like extracellular matrix protein 1 (EFEMP1) gene. PMID: 28380465
  5. MiR-338-5p suppressed proliferation, migration and invasion of glioblastoma cells through inhibiting EFEMP1. PMID: 28292024
  6. Results demonstrated that knockdown of EFEMP1 significantly inhibited ovarian cancer cell proliferation and induced cell cycle arrest at the G1/G0 phase. PMID: 27351229
  7. this review and meta-analysis shows that fibulin-3 is a useful diagnostic marker for malignant pleural mesothelioma PMID: 27769044
  8. Data suggest that HIF2alpha mediates hypoxia-induced cancer growth/metastasis and that EFEMP1 is a downstream effector of hypoxia-induced HIF2alpha during breast tumorigenesis. PMID: 27270657
  9. EFEMP1 suppresses epithelial-mesenchymal transition and migration in endometrial carcinoma PMID: 27015552
  10. Serum starvation of cultured retinal pigment epithelium cells changes the cellular distribution of cholesterol and Fibulin3 in patterns reminiscent of age-related macular degeneration. PMID: 29097185
  11. Results indicate that fibulin-3 serves as a pro-invasive factor in bladder cancer, which may be mediated through modulation of IGFBP5 expression. PMID: 28504717
  12. fibulin-3 confers a relatively high diagnostic efficacy and is acceptable to be an auxiliary biomarker to aid in malignant pleural mesothelioma identification. PMID: 28103581
  13. FBLN-3 promoter region was highly methylated in some pancreatic cancer cell lines. FBLN-3 inhibited c-MET activation and expression and reduced cellular level of ALDH1. PMID: 25451256
  14. Serum EFEMP1 is down-regulated and involved in the progression of Prostate Cancer. It may serve as a useful diagnostic biomarker, with better diagnostic accuracy than PSA in Prostate Cancer. PMID: 28085790
  15. SMRP but not FBLN3 has a role in pleural effusions in in malignant pleural mesothelioma PMID: 28314308
  16. EFEMP1 negatively modulates the migration ability in hepatocellular carcinoma. Reduction of EFEMP1 promotes the migration ability though increasing Matrix Metalloproteinase 2 and Matrix Metalloproteinase 9 expression via Extracellular Signal-Regulated MAP Kinase 1/2 activity. PMID: 27108677
  17. The EFEMP1 rs3791679 polymorphism influences glioma susceptibility in the Chinese Han population. PMID: 27706619
  18. Extensive methylation of the EFEMP1 gene promoter can discriminate invasive from benign IPMNs with superior accuracy owing to their stepwise accumulation of tumor progression PMID: 27095449
  19. The results of our study suggested an association between the EFEMP1 rs3791679 polymorphism and an elevated risk of glioma, especially in those with family history of glioma. PMID: 26823870
  20. Median FBLN-3 concentrations were 12.96. PMID: 26638921
  21. The EFEMP1 gene is significantly down-regulated in leiomyoma compared with myometrium. PMID: 26702771
  22. It was concluded that fibulin-3 is expressed at a lower level in colon cancer, and it can serve as a marker for advanced colon cancer. PMID: 26592829
  23. Novel inguinal hernia susceptibility genes are identified as EFEMP1, WT1, EBF2 and ADAMTS6. PMID: 26686553
  24. EFEMP1 might indirectly enhance the expression of MMP-2, providing a potential explanation for the role of AEG-1 in metastasis. NF-kappaB pathways might be one of the effective ways which EFEMP1 was induced by AEG-1. PMID: 25987128
  25. Suggest that FBLN-1 may play a role in arterial stiffening in patients with atherosclerosis. PMID: 26507503
  26. The polymorphisms of EFEMP1 gene were associated with breast cancer and might contribute to the susceptibility of the progression of breast cancer in Chinese Han women. PMID: 27048115
  27. Studies indicate Arg345Trp (R345W) mutation in fibulin-3 (F3) as the cause of a rare retinal dystrophy, Malattia Leventinese/Doyne Honeycomb Retinal Dystrophy (ML/DHRD). PMID: 26427406
  28. The recent finding that a common variant near EFEMP1 was associated with optic nerve-head morphology supports the possibility that the EFEMP1 variant identified in this Primary open-angle glaucoma family may be pathogenic. PMID: 26162006
  29. restoring fibulin-3 expression in breast cancer cells inhibited TGF-beta signaling, breast cancer cell EMT, invasion and metastasis in vivo. PMID: 25823021
  30. High serum Fibulin-3 levels were associated with malignant mesothelioma. PMID: 25743806
  31. EFEMP1 suppresses growth and invasion of lung cancer cells by downregulating MMP7 expression. PMID: 25676403
  32. R345W mutation in EFEMP1 is responsible for the dominant retinal drusen. PMID: 26037350
  33. The study showed that common genetic variants in EFEMP1 gene were associated with glioma and contributed to glioma susceptibility. PMID: 25638659
  34. Phenotypic assays showed that EFEMP1 de novo expression reduces malignant phenotype of PCa cells. EFEMP1 promoter methylation is prevalent in PCa and accurately discriminates PCa from non-cancerous prostate tissues and other urological neoplasms PMID: 25211630
  35. The R345W mutation in EFEMP1 caused Malattia leventinese/Doyne honeycomb retinal dystrophy in a Chinese family. PMID: 25111685
  36. R345W F3, but not WT F3, requires N-glycosylation to acquire a stable, native-like structure PMID: 25389134
  37. This study revealed that EFEMP1 SNPs could play a role in the modulation of personality traits in both healthy subjects and suicide attempters. PMID: 24241531
  38. Inhibition of ADAM10/17 or knockdown of DLL4 reduced the proangiogenic effects of fibulin-3 in culture PMID: 25139440
  39. Downregulation of fibulin-3 is associated with lung cancer invasion. PMID: 24480807
  40. Extracellular matrix proteins expression profiling in chemoresistant variants of the A2780 ovarian cancer cell line. PMID: 24804215
  41. Inhibition of gamma-secretase by RO4929097 causes at least partial sensitization of glioblastoma cells to temozolomide in vitro and in vivo. In addition, we show that EFEMP1 expression levels correlate with survival in TMZ-treated glioblastoma patients. PMID: 24495907
  42. Aberrant DNA methylation and histone H3-K9 trimethylation of EFEMP1 may be responsible for its downregulation in GC, and thus have an important role in tumor invasion and metastasis. PMID: 24718752
  43. FBLN3 suppresses both epithelial-to-mesenchymal transition and self-renewal of lung cancer stem cells by modulating the IGF1R pathway. PMID: 24013232
  44. EFEMP1 is a newly identified gene over-expressed in ovarian cancer, associated with poor clinicopathologic features and promotes angiogenesis. PMID: 24236050
  45. fibulin-3 negatively modulates the invasiveness of lung cancer cells via regulation of p38-MAPK and MMP-2/9. PMID: 24142183
  46. Subtype-independent loss of EFEMP1 expression in the majority of primary adenomas should prompt more detailed investigation in this tumor type. PMID: 24080855
  47. EFEMP1 decreased secretion of MMPs and inhibited tumor cell proliferation. PMID: 23840707
  48. Fibulin-3 exhibits antitumor effects towards hepatocellular carcinoma PMID: 23936443
  49. The top three inhibitory compounds reduced R345W fibulin-3 secretion. PMID: 23230284
  50. Define the Efemp1-p27(Kip1) axis as a potential marker module of PDAC cell sensitivity towards dual PI3K-mTOR inhibitors. PMID: 23470560

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Involvement in disease Doyne honeycomb retinal dystrophy (DHRD)
Subcellular Location Secreted, extracellular space. Secreted, extracellular space, extracellular matrix.
Protein Families Fibulin family
Tissue Specificity In the eye, associated with photoreceptor outer and inner segment regions, the nerve fiber layer, outer nuclear layer and inner and outer plexiform layers of the retina.
Database Links

HGNC: 3218

OMIM: 126600

KEGG: hsa:2202

STRING: 9606.ENSP00000347596

UniGene: Hs.732348

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