Human F-box only protein 32(FBXO32) ELISA kit

Instructions
Code CSB-EL008498HU
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name F-box protein 32
Alternative Names 4833442G10Rik ELISA Kit; AI430017 ELISA Kit; Atrogin 1 ELISA Kit; Atrogin-1 ELISA Kit; ATROGIN1 ELISA Kit; Atrophy gene 1 ELISA Kit; F box only protein 32 ELISA Kit; F-box only protein 32 ELISA Kit; F-box protein 32 ELISA Kit; FBX32_HUMAN ELISA Kit; fbxo25 ELISA Kit; FBXO32 ELISA Kit; FLJ32424 ELISA Kit; MAFbx ELISA Kit; MGC108443 ELISA Kit; MGC137646 ELISA Kit; MGC33610 ELISA Kit; Muscle atrophy F box ELISA Kit; Muscle atrophy F box protein ELISA Kit; Muscle atrophy F-box protein ELISA Kit
Abbreviation FBXO32
Uniprot No. Q969P5
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 7.8 pg/mL-500 pg/mL
Sensitivity 1.95 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cell Biology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human FBXO32 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %91
Range %87-94
1:2Average %104
Range %99-107
1:4Average %94
Range %88-97
1:8Average %98
Range %93-101
Recovery
The recovery of human FBXO32 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9387-96
EDTA plasma (n=4)9488-98
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
5002.692 2.645 2.669 2.505
2501.967 1.945 1.956 1.792
1251.215 1.197 1.206 1.042
62.50.731 0.720 0.726 0.562
31.20.433 0.444 0.439 0.275
15.60.309 0.318 0.314 0.150
7.80.243 0.251 0.247 0.083
00.165 0.163 0.164
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 5-7 working days

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Target Background

Function
(From Uniprot)
Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Probably recognizes and binds to phosphorylated target proteins during skeletal muscle atrophy. Recognizes TERF1.
Gene References into Functions
  1. F-box only protein 32 (FBXO32) directly ubiquitinates C-terminal binding protein 1 (CtBP1), which is required for its stability and nuclear retention. PMID: 29142217
  2. Authors found that the FBXO32 and SMAD4 levels were higher in normal tissues than in CRC tissues. The expressions of FBXO32 and SMAD4 were related to clinicopathological parameters in CRC. PMID: 29465067
  3. FBXO32 activates NF-kappaB through IkappaBalpha degradation in inflammatory and genotoxic stress PMID: 28970077
  4. Transcriptional analysis of endophilin-A mutant mice, complemented by proteomics, highlighted ataxia- and protein-homeostasis-related genes and revealed upregulation of the E3-ubiquitin ligase FBXO32/atrogin-1 and its transcription factor FOXO3A. PMID: 27720640
  5. Low FBXO32 expression is associated with breast cancer tumorigenesis. PMID: 28068319
  6. Our results indicate that abnormal SCF activity with subsequent impairment of the autophagic flux due to a novel FBXO32 mutation is implicated in the pathogenesis of Dilated cardiomyopathy . PMID: 26753747
  7. Our data suggest that FBXO32 is a candidate gene for recessive familial dilated cardiomyopathy. Acting as a cardiac ubiquitin ligase, mutated FBXO32 could perturb the degradation of target proteins in the ubiquitin proteasome system. PMID: 26768247
  8. Atrogin-1 expression tended to be increased in the skeletal muscle of patients with malignant disease even before cancer related cachexia weight loss. PMID: 25760630
  9. FBXO32 targets Lys-326 of c-Myc to form polyubiquitin chains, resulting in inhibition of cell proliferation. PMID: 25944903
  10. MAFbx not only regulates protein degradation, but also reduces protein synthesis, exerting a dual role in regulating cardiac mass and preventing from cardiac hypertrophy. PMID: 24650875
  11. FBXO32 methylation status and protein expression were independently associated with survival in ESCC. FBXO32 may be a functional tumor suppressor. Its inactivation through promoter methylation could play an important role in ESCC carcinogenesis. PMID: 24798237
  12. EZH2 is a key factor in the proliferation and survival of PAX3-FOXO1 alveolar RMS cells working, at least in part, by repressing FBXO32 PMID: 24213577
  13. ATROGIN1 gene expression is increased in patients with severe burn injury. PMID: 23816995
  14. Quadriceps muscle atrogin-1 levels were lower in COPD patients than controls, but similar in patients with a low and normal fat-free mass index. Atrogin1 levels were not associated with quadriceps fiber cross-sectional area or strength in patients. PMID: 23844868
  15. Data suggest expression of atrogin-1 and MuRF-1 (muscle-specific RING finger protein 1) play role in aging-related decrease in muscle mass (i.e., sarcopenia); up-regulation of atrogin-1/MuRF-1 has potential to prevent/reverse sarcopenia. [REVIEW] PMID: 22815045
  16. Cardiac and vascular atrogin-1 mRNA expression is not associated with dexamethasone efficacy in the monocrotaline model of pulmonary hypertension. PMID: 22311109
  17. In a transgenic mouse model of autoimmune myositis, cellular inflammation is associated with FOXO3A translocation and Atrogin-1 induction. PMID: 22590725
  18. MAFbx contains two functional nuclear localization signals (NLS). Mutation or deletion of only one NLS induced cytoplasmic localization of MAFbx. PMID: 22249105
  19. investigation of factors regulating expression of two ubiquitin ligases (MAFbx and MURF1) in skeletal muscle (i.e., vastus lateralis): effects of resistance exercise and anabolic dietary supplement (i.e., branched-chain amino acids) PMID: 22127230
  20. SerpinB5 interacts with KHDRBS3 and FBXO32, and KHDRBS3 can interact with FBXO32 mRNA. PMID: 21725612
  21. atrophic AKT-FOXO signaling play major roles in eliciting pathological changes associated with diaphragm disuse. PMID: 20833824
  22. The novel tumor suppressor FBXO32 is epigenetically silenced in ovarian cancer cell lines with disrupted TGF-beta/SMAD4 signaling, and FBXO32 methylation status predicts survival in patients with ovarian cancer. PMID: 20065949
  23. Human skeletal muscle atrophy in the amyotrophic lateral sclerosis shows an increase in atrogin-1 & a decrease in Akt. The transcriptional regulation of human atrogin-1 may be controlled by an Akt-mediated transcription factor other than FKHR. PMID: 16507768
  24. results suggest that Cbl-b- or atrogin-1-mediated ubiquitination plays an important role in unloading-induced muscle atrophy, and that unloading stress may preferentially inhibit transcriptional responses in skeletal muscle PMID: 16868939
  25. Atrogin-1 mRNA expression was significantly increased in quadriceps of patients with COPD; transcriptional regulation of atrogin-1 and MuRF1 may occur via FoxO-1, but independently of AKT PMID: 17478621
  26. CSRP3, MUSTN1, SIX1, and FBXO32 expression changes in response to lengthening and shortening contractions in human muscle PMID: 17519359
  27. We conclude that smoking impairs the muscle protein synthesis process and increases the expression of genes associated with impaired muscle maintenance; smoking therefore likely increases the risk of sarcopenia. PMID: 17609255
  28. Results showed upregulation of MuRf1 and MAFbx in atrophies muscle and support their role as regulatory peptides in various conditions which lead to muscle atrophy. PMID: 17977773
  29. atrogin-1 may be a critical mediator of the muscle damage induced by statins. PMID: 17992259
  30. Testosterone represses MAFbx expression via interactions of the AR with Oct-1. PMID: 18599544
  31. Review discusses findings implicating atrogin-1, a gene required for muscle atrophy, in the pathophysiology of statin-induced muscle injury. PMID: 18681786

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Involvement in disease Defects in FBXO32 are associated with susceptibility to dilated cardiomyopathy (DCM). A disorder characterized by ventricular and impaired systolic function, resulting in heart failure and arrhythmia. Patient are at risk of premature death.
Subcellular Location Cytoplasm, Nucleus
Tissue Specificity Specifically expressed in cardiac and skeletal muscle.
Database Links

HGNC: 16731

OMIM: 606604

KEGG: hsa:114907

STRING: 9606.ENSP00000428205

UniGene: Hs.403933

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