Human Interleukin 12,IL-12/P40 ELISA KIT

Instructions
Code CSB-E04598h
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40)
Alternative Names CLMF ELISA Kit; CLMF p40 ELISA Kit; CLMF2 ELISA Kit; Cytotoxic lymphocyte maturation factor 40 kDa subunit ELISA Kit; IL 12B ELISA Kit; IL-12 subunit p40 ELISA Kit; IL-12B ELISA Kit; IL12 p40 ELISA Kit; IL12B ELISA Kit; IL12B_HUMAN ELISA Kit; IMD28 ELISA Kit; IMD29 ELISA Kit; Interleukin 12 beta chain ELISA Kit; Interleukin 12 p40 ELISA Kit; Interleukin-12 subunit beta ELISA Kit; NK cell stimulatory factor chain 2 ELISA Kit; NKSF ELISA Kit; NKSF2 ELISA Kit
Abbreviation IL12B
Uniprot No. P29460
Species Homo sapiens (Human)
Sample Types serum, plasma, cell culture supernates, urine, tissue homogenates
Detection Range 7.8 pg/mL-500 pg/mL
Sensitivity 1.95 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human IL-12/P40 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 94  
Range % 90-98  
1:2 Average % 102  
Range % 97-105  
1:4 Average % 89  
Range % 84-92  
1:8 Average % 98  
Range % 91-101  
Recovery
The recovery of human IL-12/P40 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 91 85-96  
EDTA plasma (n=4) 103 97-106  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
500 2.629 2.589 2.609 2.475  
250 1.664 1.631 1.648 1.514  
125 1.042 1.087 1.065 0.931  
62.5 0.688 0.672 0.680 0.546  
31.2 0.438 0.444 0.441 0.307  
15.6 0.283 0.293 0.288 0.154  
7.8 0.201 0.214 0.208 0.074  
0 0.135 0.133 0.134    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Data

Function Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated killer cells, and stimulate the production of IFN-gamma by resting PBMC.
Gene References into Functions
  1. Effect of polymorphisms in IL-12B p40, IL-17A and IL-23 A/G genes on the response of psoriatic patients to narrowband UVB. PMID: 29763989
  2. showed that IL-12B A1188C (rs3212227) can contribute to the progression of the disease in the Czech population PMID: 30069682
  3. Study confirms an association between IL12B and IL23R genetic polymorphism and psoriasis vulgaris (with a protective effect of minor alleles). PMID: 29454820
  4. IL-12 B gene AA genotype presented more frequently in late stages of Hepatitis C virus chronically ill patients, while, CC genotype had no significant association with staging of liver disease and had low frequency especially in late stages of liver disease. PMID: 28595541
  5. Frequency of IL-12B AA (rs6871626) genotype was increased in Ankylosing Spondylitis patients. Interleukin IL-12B AA (rs6871626) gene polymorphisms could serve as promising biomarkers for diagnosis and prognosis in Ankylosing Spondylitis patients. PMID: 29200018
  6. The demonstrated allelic expression imbalance supports the idea that the IL12B risk haplotype confers susceptibility not only to Crohn's disease onset but to also relapse through increased IL12B mRNA expression. PMID: 28229296
  7. the present study IL-12B gene status was evaluated in 50 new sputum smear-positive pulmonary tuberculosis patients. PMID: 28697396
  8. The rs17860508 polymorphism in the IL12B promoter region may influence the risk of developing ovarian endometriosis by altering the endometrial expression of IL12B of in Northern Chinese women. PMID: 29738836
  9. The rs6871626 single nucleotide polymorphism in IL-12B is associated with the pathophysiology of Takayasu arteritis. PMID: 28874185
  10. Human neutrophil elastase is involved in transactivation of TLR4 through activation of DUOX-2/EGFR and synergistically enhances IL-12p40 production by macrophages stimulated with LPS. PMID: 27282560
  11. our results suggested a significant association between IL-12B 3'-UTR and rs6887695 SNPs and ADs. PMID: 27068848
  12. Single-nucleotide-polymorphisms of IL12B may be considered a high-risk factor for Takayasu arteritis in Chinese Han population PMID: 28160070
  13. IL-12B -1188 C allele may be a protective factor against asthma in East Asians PMID: 28287286
  14. The low expression of HDAC3 and overexpession of inflammatory cytokines (IL-18, IL-12 and TNF-alpha) in intrahepatic cholestasis of pregnancy may be involved in liver cell apoptosis and in the pathophysiology of the disease. PMID: 28697498
  15. Identification of PARP-1 as a unique regulator of Il12b transcription in response to inflammatory insults in an allele-differentiating manner PMID: 28219892
  16. Pre-incubation with IL-12 was able to restore IFN-gamma production and the cytotoxicity capacities of NK cells. This cytokine may therefore be considered as a potential treatment candidate in traumatic brain injury patients with immune suppression. PMID: 26387630
  17. Visceral leishmaniasis in two patients with IL-12p40 and IL-12Rbeta1 deficiencies PMID: 27873456
  18. NOD2 rs3135500 and IL12B rs1368439 SNPs were not genetic risk factors for colorectal cancer in the studied Iranian population. PMID: 27426943
  19. IL-12B gene polymorphisms were not related with rheumatoid arthritis (RA). However, rs6887695 was associated with RA in Asian patients PMID: 27155343
  20. Suggest Il12B SNPs as genetic marker of disease severity in Takayasu arteritis severity. PMID: 25783557
  21. The results of this study suggested that rs6887695 SNP in IL12B gene increase susceptibility to relapsing-remitting multiple sclerosis. PMID: 28276258
  22. these meta-analyses demonstrated that IL-12B rs3212227 and rs6887695 polymorphisms do not confer susceptibility to rheumatoid arthritis [data synthesis] PMID: 27312970
  23. Findings indicate that IL-12p40 + 1188A/C polymorphism as well as IL-12p70 protein levels may be associated with rheumatoid arthritis (RA) in the Polish population. PMID: 27896842
  24. The present meta-analysis suggests that the IL12B +1188A/C (rs3212227) polymorphism might be associated with genetic susceptibility to autoimmune diseases, such as type 1 diabetes, rheumatoid arthritis and Behcet disease, but not Graves disease and ankylosing spondylitis. PMID: 26915668
  25. this study shows that IL-12B gene polymorphism does not contribute to the risk of hepatocellular carcinoma on top of chronic hepatitis C virus infection in Egyptian patients PMID: 27819525
  26. this study has demonstrated (i) a significant association of -1082GG IL-10 genotype with susceptibility to autoimmune thyroid disease and (ii) the concomitant presence of IL-12B +1188CC and IL-10-1082 GG genotype contributes to the development and progression of Hashimoto's thyroiditis PMID: 27774749
  27. Il-12B SNPs have an association with anti-HBs Ab production after vaccination in healthy Korean infants. PMID: 28051794
  28. Our results reveal that the IL12A rs568408 variant may be a marker SNP for risk of both HBV clearance and HBV-related hepatocellular carcinoma development PMID: 26631030
  29. this study shows that the Il-12 genetic polymorphisms is associated with susceptibility to tuberculosis in patients and their household contacts in India PMID: 27108964
  30. High IL12 levels can inhibit Candida albicans colonizing the gastrointestinal tract of children and adolescents with diabetes mellitus type 1. PMID: 28127111
  31. this study shows that IL-12B gene polymorphism is genetic risk factor for systemic lupus erythematosus in the Polish population, and that it predicts disease phenotype PMID: 27059274
  32. analysis of cytokine gene polymorphisms suggests that IL-12 gene may play impact on specific pathogenesis of ophthalmopathy in Korean children with early onset autoimmune thyroid disease. PMID: 26850223
  33. IL-12 polymorphism rs3212227 might not be a critical risk factor for Preeclampsia in Chinese Han women. PMID: 27148908
  34. a tendency of a higher prevalence of the genotype IL-12p40 pro1.1 in systemic arthritis and in rheumatoid factor negative polyarthritis was observed but not significant PMID: 26667304
  35. genetic polymorphism is associated with psoriasis in the South Indian Tamils PMID: 26472011
  36. genetic polymorphism is associated with allergic rhinitis in Chinese patients PMID: 26663019
  37. The IL23R polymorphisms rs10889677, rs7517847, and the IL12B polymorphism rs3212227 are not associated with multiple sclerosis risk. PMID: 26000455
  38. IL-12 immunomodulation delays the onset of lethal peritoneal disease of ovarian cancer. PMID: 26430781
  39. variant IL-12p40 1188C/A genotype was AA (72.92%), AC (23.96%), and CC (3.13%%) in patients as compared to 65%, 30%, and 5%, respectively, in controls. PMID: 26516307
  40. the IL-12B rs3212227 AC and AC+CC genotypes are associated with rheumatoid arthritis risk in older patients,rheumatoid factor positive patients and anti-cyclic peptide antibodies negative patients PMID: 26375522
  41. High serum IL-12 levels associated with increased densities of peritumoral CD8(+) T cells, intraepithelial CD3(+) T cells and intratumoral neutrophils, while high serum CCL4 levels associated with increased densities of peritumoral CD68(+) cells PMID: 26874795
  42. These observations indicate a HDAC1-mediated IL-12B gene expression suppression by live, virulent Mycobacterium tuberculosis to subvert the immune system to survive and replicate in the host. PMID: 26697414
  43. IL-12p40 response to in vitro stimulation in patients with Mendelian susceptibility to mycobacterial disease was below normal levels. PMID: 25201764
  44. 8 SNPs (rs10045431, rs11167764, rs3212227, rs6556412, rs6556416, rs6871626, rs6887695 and rs7709212) were genotyped in Han ankylosing spondylitis patients and controls. rs6871626 may be associated AS susceptibility and activity. PMID: 26103568
  45. Replicating the association of single-nucleotide polymorphisms in the TNFAIP3, IL12B and IL23R genes with psoriasis vulgaris, in subjects from different ethnic backgrounds, underlines their importance in the pathogenesis of the disease. PMID: 25406098
  46. balance of IL-23 vs IL-12/IL-27 signals into CD4(+) effector T cells determines whether tissue inflammation is perpetuated or resolves PMID: 24796719
  47. Compared with the IL-12B-AA genotype, CC and combined CC/AC genotypes were associated with a significant decreased risk of Hepatitis C virus infection in Chinese hemodialysis patients. PMID: 25613737
  48. These results show that IL-15 and IL-12 combination has the ability to expand the selective depletion of invariant natural killer T cells in vitro in HIV-infected individuals. PMID: 24748538
  49. The risk of gastric cardiac adenocarcinoma associated with the IL12B rs3212227 T > G polymorphism was evident among Chinese female patients and Chinese patients who never smoked or consumed alcoholic drinks. PMID: 24529168
  50. Based on these results, we believe that the increased levels of IL-12p40 and IL-16 are associated with an ongoing inflammatory response in obese individuals and could lead to the development of disease conditions related to obesity. PMID: 25710036

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Involvement in disease Immunodeficiency 29 (IMD29); Psoriasis 11 (PSORS11)
Subcellular Location Secreted
Protein Families Type I cytokine receptor family, Type 3 subfamily
Database Links

HGNC: 5970

OMIM: 161561

KEGG: hsa:3593

STRING: 9606.ENSP00000231228

UniGene: Hs.674

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