Product Details

Target Name

keratin 6A

Alternative Names

CK-6A ELISA Kit; CK-6D ELISA Kit; CK6A ELISA Kit; CK6C ELISA Kit; CK6D ELISA Kit; Cytokeratin-6A ELISA Kit; Cytokeratin-6D ELISA Kit; K2C6A_HUMAN ELISA Kit; K6A ELISA Kit; K6C ELISA Kit; K6D ELISA Kit; keratin 6A ELISA Kit; Keratin ELISA Kit; Keratin; type II cytoskeletal 6A ELISA Kit; Keratin-6A ELISA Kit; Krt6a ELISA Kit; KRT6C ELISA Kit; KRT6D ELISA Kit; type II cytoskeletal 6A ELISA Kit; Type-II keratin Kb6 ELISA Kit

Abbreviation

KRT6A

Uniprot No.

P02538

Species

Homo sapiens (Human)

Sample Types

serum, plasma, tissue homogenates, cell lysates

Detection Range

0.156 ng/mL-10 ng/mL

Sensitivity

0.039 ng/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Signal Transduction

Assay Principle

quantitative

Measurement

Sandwich

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human KRT6A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	87
	Range %	82-91
1:2	Average %	84
	Range %	80-88
1:4	Average %	103
	Range %	98-108
1:8	Average %	90
	Range %	85-94

Recovery

The recovery of human KRT6A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	98	94-103
EDTA plasma (n=4)	94	90-100

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
10	2.098	2.102	2.100	1.955
5	1.721	1.793	1.757	1.612
2.5	1.382	1.421	1.402	1.257
1.25	1.090	1.150	1.120	0.975
0.625	0.695	0.714	0.705	0.560
0.312	0.459	0.436	0.448	0.303
0.156	0.267	0.262	0.265	0.120
0	0.142	0.147	0.145

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx

Description

This Human KRT6A ELISA Kit was designed for the quantitative measurement of Human KRT6A protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 0.156 ng/mL-10 ng/mL and the sensitivity is 0.039 ng/mL.

Code	CSB-EL012561HU
Size	96T,5×96T,10×96T
Price	Request a Quote or Start an on-line Chat
Trial Size	24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Human Keratin, type II cytoskeletal 6A(KRT6A) ELISA kit

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