Human Membrane-associated progesterone receptor component 1(PGRMC1) ELISA kit

Code CSB-EL017876HU
Size 96T,5×96T,10×96T
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Product Details

Target Name
progesterone receptor membrane component 1
Alternative Names
PGRMC1; HPR6.6; PGRMC; Membrane-associated progesterone receptor component 1; mPR; Dap1; IZA
Abbreviation
PGRMC1
Uniprot No.
Species
Homo sapiens (Human)
Sample Types
serum, plasma, tissue homogenates, cell lysates
Detection Range
47 pg/mL-3000 pg/mL
Sensitivity
11.7 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Cancer
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human PGRMC1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %92
Range %85-98
1:2Average %96
Range %90-102
1:4Average %93
Range %85-99
1:8Average %89
Range %80-94
Recovery
The recovery of human PGRMC1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 10096-106
EDTA plasma (n=4)9590-100
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
30002.124 2.198 2.161 2.046
15001.409 1.415 1.412 1.297
7500.923 0.951 0.937 0.822
3750.588 0.590 0.589 0.474
187.50.390 0.384 0.387 0.272
940.274 0.286 0.280 0.165
470.197 0.190 0.194 0.079
00.114 0.116 0.115  
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Human PGRMC1 ELISA Kit was designed for the quantitative measurement of Human PGRMC1 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 47 pg/mL-3000 pg/mL and the sensitivity is 11.7 pg/mL .

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Target Background

Function
(From Uniprot)
Component of a progesterone-binding protein complex. Binds progesterone. Has many reported cellular functions (heme homeostasis, interaction with CYPs). Required for the maintenance of uterine histoarchitecture and normal female reproductive lifespan. Intracellular heme chaperone. Regulates heme synthesis via interactions with FECH and acts as a heme donor for at least some hemoproteins.
Gene References into Functions
  1. High PGRMC1 expression is associated with renal cell carcinoma. PMID: 30015972
  2. Down-regulation of PGRMC1 was associated with poor tumor differentiation and worse patient survival in hepatocellular carcinoma. PMID: 29563759
  3. These new findings demonstrate that PGRMC1 might play an important role in progression and therapy resistance of human breast tumors and could offer an interesting target for anticancer therapy. PMID: 28701017
  4. Cystathionine beta-synthase and PGRMC1 function as carbon monoxide sensors in health and disease. (Review) PMID: 27565814
  5. Sigma-2 receptor and PGRMC1 are not identical. PMID: 28007569
  6. Report a dynamic pattern of PGRMC1 and SERBP1 expression in human endometrium throughout the menstrual cycle and highlight a putative novel implication of PGRMC1 in the human decidualization process. PMID: 28911927
  7. Progesterone, Inflammatory Cytokine (TNF-alpha), and Oxidative Stress (H2O2) Regulate Progesterone Receptor Membrane Component 1 (PGRMC1) Expression in Fetal Membrane Cells. Study concludes that these stimuli act as regulatory factors of PGRMC1 in a cell-specific manner. PMID: 26919974
  8. findings demonstrate PGRMC1, which promotes RCC progression phenotypes in vitro and in vivo, is a novel potential biomarker and therapeutic target for RCC PMID: 28107520
  9. Data suggest that PGRMC1 plays important roles in synthesis of cholesterol and steroids and in energy regulation. [REVIEW] PMID: 28104494
  10. data illustrate a role for PGRMC1 in regulating heme synthesis via interactions with FECH and suggest that PGRMC1 may be a heme chaperone or sensor PMID: 27599036
  11. The effects of progesterone treatment on the expression levels of progesterone receptor membrane component 1 (PGRMC1), plasminogen activator inhibitor 1 RNA-binding protein (PAIRBP1), and progestin and adipoQ receptor 7 (PAQR7) on both mRNA and protein levels were investigated in spheroids derived from human glioma cell lines U-87 MG and LN-229. PMID: 27340667
  12. It can be suggested that women with breast epithelium highly expressing PGRMC1 and in interaction with ERalpha may have an increased risk to develop breast cancer. PMID: 26303031
  13. These results clarify that PGRMC1 and the S2R are distinct binding sites expressed by different genes. PMID: 26870805
  14. Haem-mediated PGRMC1 dimerization is required for interactions with EGFR and cytochromes P450, cancer proliferation and chemoresistance against anti-cancer drugs. PMID: 26988023
  15. indicate that PGRMC1 regulates hepcidin gene expression through an evolutionarily conserved mechanism PMID: 26657863
  16. results suggested that PGRMC1 and sigma2 receptors are two different molecular entities. PMID: 25843410
  17. PGRMC1 is the major progesterone receptor eliciting the protective effects of Norgestrel, a synthetic female progesterone hormone. PMID: 26447367
  18. coding mutations of PGRMC1 do not seem to be a common cause of the disease in Han Chinese PMID: 25246111
  19. PGRMC1 overexpression led to the epithelial-mesenchymal transition and chemoresistance in uterine sarcoma. PMID: 25596698
  20. progesterone binds to hPGRMC1 and introduces spectral changes that manifest conformational changes to the heme. PMID: 25675345
  21. Loss of PGRMC1 expression is associated with endometrial tumor cell viability during chemotherapeutic stress. PMID: 25304370
  22. PGRMC1 binds to key components of the autophagy machinery and is required for the degradative activity of autophagy PMID: 24113030
  23. PGRMC1 expression appears to be diminished in pregnant women with preterm premature rupture of the membranes. PMID: 24680695
  24. PGRMC-1 expression is downregulated in the endometria from women with advanced stage endometriosis. PMID: 23793472
  25. In a chorion cell line, PGRMC1 mediates the effects of progestins on matrix metalloproteinase 9 activity. PMID: 23813454
  26. PGRMC1 can act as an adaptor protein for multiple classes of steroid receptors PMID: 24424068
  27. ABHD5, PGRMC1 and SQS are novel markers for sebaceous carcinoma and can reliably distinguish sebaceous neoplasms from non-sebaceous tumors, specifically BCC with clear cell features. PMID: 23557589
  28. The expression of PGRMC1 is strongly associated with the progression of breast cancer. PMID: 22588913
  29. Data suggest that women with breast cells overexpressing PGRMC1 have higher risk of breast cancer when treated with progestins (as in some hormonal contraceptives). PMID: 23116217
  30. miR-98 and miR-181a through their regulatory functions on PGRMC1, PGR, CYP19A1, TIMP3, and DDX3X expression may influence a wide range of endometrial cellular activities during normal menstrual cycle and transition into disease states. PMID: 22492871
  31. Pgrmc1 localizes to secretory vesicles in cancer cells, and Pgrmc1 was secreted by lung cancer cells. Furthermore, Pgrmc1 was significantly elevated in the plasma of lung cancer patients compared to noncancer patients. PMID: 21918976
  32. PGRMC1 negatively modulates the drug-metabolizing activities of P450. PMID: 21825115
  33. In patients with elevated PGRMC1 mRNA levels, gonadotropin-induced follicle development is attenuated, although sufficient numbers of follicles develop to allow for embryo and subsequent pregnancy. PMID: 22245528
  34. roles of progesterone (P4) and Progesterone Receptor Membrane Component 1 (PGRMC1) in regulating mitosis of ovarian cancer cells, SKOV-3. P4's actions appear to be dependent on PGRMC1's function within the mitotic spindle. PMID: 21148105
  35. Expression of PGRMC1 mRNAs was significantly lower in JEG-3 cells compared to non-syncytialized BeWo cells. PMID: 21455559
  36. PGRMC1 is lower in myometrium of women at term either not in labour (P = 0.004) or in labour (P = 0.005) compared with tissues from women in preterm non-labour. PMID: 21131300
  37. we propose that PGRMC1 expression is regulated by the miRNAs let-7/miR-98 PMID: 21109987
  38. Reduced levels of PGRMC1 in peripheral leukocytes are associated with perturbed ovulatory function. PMID: 20537145
  39. Pgrmc1 associates with epidermal growth factor receptor and regulates erlotinib sensitivity PMID: 20538600
  40. increases in vivo tumor growth, anchorage-independent growth, and migration PMID: 20164297
  41. Hpr6.6 regulates cell death through a novel oxidative damage response pathway. PMID: 14523988
  42. The expression of a novel progesterone-binding protein (hmPR2/PGMRC2) was investigated. Transcripts of this protein have been detected in human sperm. PMID: 15702432
  43. Hpr6 homologues regulate cytochrome P450 proteins implicated in hormone, steroid and xenobiotic chemical metabolism; Hpr6 expression linked to cancer progression and cellular survival PMID: 15970648
  44. Our findings support a model in which Hpr6 binds to heme and regulates susceptibility to damaging agents PMID: 16234411
  45. These findings demonstrate that PGRMC1 is required for P450 activity and suggest that interindividual variation in PGRMC1 function may impact multiple biochemical pathways and drug metabolism. PMID: 17276356
  46. PGRMC1 may be involved in sterol metabolism or homeostasis and cell survival [review] PMID: 18249431
  47. PGRMC1 plays an important role in promoting ovarian cancer cell viability and that attenuating PGRMC1's action makes the ovarian cancer cells more sensitive to cisplatin. PMID: 18319313
  48. The aim of this study was to assess the expression, localization and hormonal regulation of two novel P(4) receptor candidates, P(4) receptor membrane component (PGRMC) 1 and PGRMC2, as well as the PGRMC1 partner Serpine 1 mRNA binding protein (SERBP1). PMID: 18440126
  49. The results demonstrate the first demonstration of mPR expression in human epithelial ovarian tumors. PMID: 18479732
  50. These findings suggest that mutant or reduced levels of PGMRC1 may cause premature ovarian failure through impaired activation of the microsomal cytochrome P450 and increased apoptosis of ovarian cells. PMID: 18782852

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Subcellular Location
Microsome membrane; Single-pass membrane protein. Smooth endoplasmic reticulum membrane; Single-pass membrane protein. Mitochondrion outer membrane; Single-pass membrane protein; Extracellular side.
Protein Families
Cytochrome b5 family, MAPR subfamily
Tissue Specificity
Widely expressed, with highest expression in liver and kidney. Expressed by endometrial glands and stroma (at protein level).
Database Links

HGNC: 16090

OMIM: 300435

KEGG: hsa:10857

STRING: 9606.ENSP00000217971

UniGene: Hs.90061

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