Human NADH-ubiquinone oxidoreductase chain 6(MT-ND6) ELISA kit

Code CSB-EL015082HU
Size 96T,5×96T,10×96T How to order?
Trial Size 24T ELISA kits trial application
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Product Details


This Human MT-ND6 ELISA Kit was designed for the quantitative measurement of Human MT-ND6 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 23.44 pg/mL-1500 pg/mL and the sensitivity is 5.86 pg/mL.

Target Name mitochondrially encoded NADH dehydrogenase 6
Alternative Names MT-ND6 ELISA Kit; MTND6 ELISA Kit; NADH6 ELISA Kit; ND6 ELISA Kit; NADH-ubiquinone oxidoreductase chain 6 ELISA Kit; EC ELISA Kit; NADH dehydrogenase subunit 6 ELISA Kit
Abbreviation MT-ND6
Uniprot No. P03923
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 23.44 pg/mL-1500 pg/mL
Sensitivity 5.86 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Sandwich
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
To assess the linearity of the assay, samples were spiked with high concentrations of Human MT-ND6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
1:1Average %92
Range %84-98
1:2Average %86
Range %80-92
1:4Average %94
Range %85-98
1:8Average %102
Range %98-106
The recovery of Human MT-ND6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9488-98
EDTA plasma (n=4)8983-95
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
15002.763 2.867 2.815 2.716
7502.426 2.321 2.374 2.275
3751.675 1.772 1.724 1.625
187.51.045 1.115 1.080 0.981
93.750.527 0.534 0.531 0.432
46.880.274 0.277 0.276 0.177
23.440.188 0.178 0.183 0.084
00.098 0.099 0.099
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
and FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Background

(From Uniprot)
Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) which catalyzes electron transfer from NADH through the respiratory chain, using ubiquinone as an electron acceptor. Essential for the catalytic activity and assembly of complex I.
Gene References into Functions
  1. Study identify a significant down-expression of MT-ND6 in men with Klinefelter syndrome. PMID: 29333085
  2. The molecular diagnostics by the whole exome sequencing showed a novel de novo (c.1374-2A>C) mutation in the KRT10 gene responsible for the development of IWC (KRT10 defect was confirmed by immunofluorescent study). Concurrently, the m.14484T>C mutation in mitochondrial MTND6 gene (characteristic for Leber's hereditary optic neuropathy or LHON) was detected in patient, his mother and brother PMID: 28944608
  3. This review focuses on the role of mitochondrial genes in causing LHON and therapeutics available for treating the disease. A systematic search has been adopted in various databases using the keywords "LHON," "mitochondria," "ND1," "ND4," "ND6," and "therapy" and the following review on mitochondrial genetics PMID: 29133631
  4. Liver MT-ND6 transcriptional activity and protein expression were decreased in nonalcoholic steatohepatitis, suggesting that the expression of this mitochondrial gene may play an important role in the disease progression. PMID: 22879518
  5. This study shows that m.14484T>C arose independently in a different mtDNA haplogroup and ethnic background in India, which may influence the clinical expression of the disease. PMID: 23674761
  6. These data suggested that the ND6 T14502C variant may modulate the phenotypic manifestation of the G11778A mutation in these Chinese pedigrees. PMID: 20691156
  7. the G14459A mutation is a candidate mutation for maternally inherited dystonia PMID: 20052369
  8. novel mitochondrial DNA nucleotide transversion, C14482A (M64I) was found to cause Leber's hereditary optic neuropathy with visual recovery PMID: 12112086
  9. mitochondrial ND6 may have a role in Leber's hereditary optic neuropathy PMID: 15922297
  10. Free radicals-mediated damage was studied in transmitochondrial cells harboring T14487C mutation in the ND6 gene of mtDNA. PMID: 16337195
  11. Therefore, the coexistence of the A1555G mutation and T14484C mutations in this Chinese family indicate that the A1555G mutation may play a synergistic role in the phenotypic manifestation of LHON associated ND6 T14484C mutation. PMID: 17452034
  12. T14484C and T14502C in ND6 gene are associated with Leber's hereditary optic neuropathy in a Chinese family. PMID: 18440284
  13. Molecular analysis has led to identification of the known T14502C mutation in ND6 gene in Chinese families with Leber's hereditary optic neuropathy. PMID: 19732751
  14. missense mutation 14487T>C resulted in severe encephalopathies ranging from infantile Leigh syndrome to adult-onset progressive myoclonic epilepsy with dystonia in a five-generation family PMID: 20019223

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Involvement in disease Leber hereditary optic neuropathy (LHON); Leber hereditary optic neuropathy with dystonia (LDYT); Mitochondrial encephalomyopathy with lactic acidosis and stroke-like episodes syndrome (MELAS); Mitochondrial complex I deficiency (MT-C1D)
Subcellular Location Mitochondrion inner membrane; Multi-pass membrane protein.
Protein Families Complex I subunit 6 family
Database Links

HGNC: 7462

OMIM: 252010

KEGG: hsa:4541

STRING: 9606.ENSP00000354665

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