Human Obestatin ELISA Kit

Citations (4) Protocol MSDS

Code	CSB-E12818h
Size	96T,5×96T,10×96T
Price	Request a Quote
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Product Details
Citations (4)
Customer Reviews and Q&A

Product Details

Target Name

Obestatin

Abbreviation

Obestatin

Species

Homo sapiens (Human)

Sample Types

serum, plasma, cell culture supernates, urine, tissue homogenates

Detection Range

4.69 pg/mL-300 pg/mL

Sensitivity

1.17 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Signal Transduction

Assay Principle

quantitative

Measurement

Sandwich

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human Obestatin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

Recovery

The recovery of human Obestatin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.

Description

The Human Obestatin ELISA Kit is designed to detect and quantify the Obestatin protein in human samples. Obestatin is a hormone that has been linked to various physiological functions, making our ELISA kit an essential tool for researchers working in the field of Signal Transduction and metabolism research.

This ELISA kit can be used for the detection of Obestatin in human serum, plasma, cell culture supernates, urine, and tissue homogenates. With a detection range of 4.69 pg/mL-300 pg/mL and a sensitivity of 1.17 pg/mL, our kit provides highly accurate and reliable results, allowing you to obtain precise measurements of Obestatin in your samples.

The assay time ranges from 1-5 hours, and only requires a sample volume of 50-100ul, making our ELISA kit a quick and efficient solution for your research needs. The detection wavelength of 450 nm provides clear and reliable readings.

This Human Obestatin ELISA Kit operates using a sandwich assay principle, which ensures accurate and precise measurements of Obestatin in your samples. The kit's high sensitivity and specificity make it a must-have tool for researchers looking to investigate the role of Obestatin in various physiological functions.

Citations

Plasma levels of obestatin and ghrelin and obestatin/ghrelin ratio in obese patients M Sfar,ResearchGate,2023
Serum ghrelin and obestatin levels in patients with acne vulgaris: are they important for the severity? Zekiye Kanat, et al,Postepy Dermatologii I Alergologii,2019
Acute effect of smoking and smoking abstinence on energy intake and appetite-related hormones blood concentrations Yannakoulia M.et al,Physiol Behav,2017
Is association between thyroid hormones and gut peptides, ghrelin and obestatin, able to suggest new regulatory relation between the HPT axis and gut? Emami A et al,Regul Pept,2014

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