Human Superoxide dismutase [Cu-Zn] (SOD1) ELISA kit

Instructions
Code CSB-E16845h
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application

Product Details

Target Name superoxide dismutase 1, soluble
Alternative Names ALS, ALS1, IPOA, SOD, homodimer, Cu/Zn superoxide dismutase|SOD, soluble|indophenoloxidase A|superoxide dismutase, cystolic
Abbreviation SOD1
Uniprot No. P00441
Species Homo sapiens (Human)
Sample Types serum, plasma, homogenates
Detection Range 62.5 pg/ml - 4000 pg/ml
Sensitivity 15.6 pg/ml
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Metabolism
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human SOD1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1000 Average % 85  
Range % 80-95  
1:2000 Average % 97  
Range % 91-102  
1:4000 Average % 97  
Range % 92-110  
1:8000 Average % 95  
Range % 86-98  
Recovery
The recovery of human SOD1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 95 89-98  
EDTA plasma (n=4) 95 90-101  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
1000 2.483 2.513 2.498 2.410  
500 1.876 1.895 1.886 1.798  
250 1.270 1.294 1.282 1.194  
125 0.756 0.741 0.749 0.661  
62.5 0.512 0.502 0.507 0.419  
31.2 0.288 0.274 0.281 0.193  
15.6 0.211 0.212 0.212 0.124  
0 0.085 0.091 0.088    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days
Still Have Questions? Leave a Message or Start an on-line Chat

Target Data

Function Destroys radicals which are normally produced within the cells and which are toxic to biological systems.
Involvement in disease Amyotrophic lateral sclerosis 1 (ALS1)
Subcellular Location Cytoplasm, Mitochondrion, Nucleus
Protein Families Cu-Zn superoxide dismutase family
Database Links

HGNC: 11179

OMIM: 105400

KEGG: hsa:6647

STRING: 9606.ENSP00000270142

UniGene: Hs.443914

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