Code | CSB-RA829583A0HU |
Size | US$210 |
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Application | Recommended Dilution |
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WB | 1:500-1:5000 |
FC | 1:20-1:200 |
The SOD1 recombinant monoclonal antibody can be used to detect human SOD1 protein in ELISA, WB, and FC applications. It is produced using recombinant DNA technology, starting with the synthesis of the gene coding for the SOD1 monoclonal antibody after sequencing the cDNA of the SOD1 antibody-producing hybridomas. These hybridomas are generated by fusing B cells from an animal immunized with a synthesized peptide derived from human SOD1 with myeloma cells. After cloning the synthesized gene into a vector, the recombinant vector is transfected into cells for cultivation. The resulting SOD1 recombinant monoclonal antibody is purified using affinity chromatography from the cell culture supernatant.
The SOD1 protein is an antioxidant enzyme that plays a key role in protecting cells from oxidative stress. It works by converting superoxide radicals, which are highly reactive molecules that can damage cells, into less harmful molecules. SOD1 is found in the cytoplasm of cells and is particularly abundant in the brain and spinal cord, where it helps protect neurons from oxidative damage. Mutations in the SOD1 gene are associated with the development of familial amyotrophic lateral sclerosis (ALS).
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