Human Thyroxine-Binding Globulin,TBG ELISA Kit

Code CSB-E09501h
Size 96T,5×96T,10×96T
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Product Details

Target Name
serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 7
Alternative Names
Serine (or cysteine) proteinase inhibitor clade A (alpha 1 antiproteinase antitrypsin) member 7 ELISA Kit; Serpin A7 ELISA Kit; Serpin peptidase inhibitor clade A (alpha 1 antiproteinase antitrypsin) member 7 ELISA Kit; Serpin peptidase inhibitor, clade A, member 7 ELISA Kit; Serpina7 ELISA Kit; T4 binding globulin ELISA Kit; TBG ELISA Kit; TBG, serum ELISA Kit; THBG_HUMAN ELISA Kit; Thyroxin binding globulin ELISA Kit; Thyroxine binding globulin ELISA Kit; Thyroxine-binding globulin ELISA Kit; Thyroxine-binding globulin od serum ELISA Kit
Abbreviation
SERPINA7
Uniprot No.
Species
Homo sapiens (Human)
Sample Types
serum, plasma, tissue homogenates
Detection Range
12.5 ng/mL-200 ng/mL
Sensitivity
12.5 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Others
Assay Principle
quantitative
Measurement
Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<15%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<15%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human TBG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 91  
Range % 85-96  
1:2 Average % 96  
Range % 92-105  
1:4 Average % 84  
Range % 80-89  
1:8 Average % 91  
Range % 85-98  
Recovery
The recovery of human TBG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 91 87-95  
EDTA plasma (n=4) 96 90-102  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average    
200 0.121 0.115 0.118    
100 0.328 0.328 0.328    
50 0.513 0.312 0.413    
25 1.015 0.497 0.756    
12.5 2.116 2.121 2.119    
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Human SERPINA7 ELISA Kit was designed for the quantitative measurement of Human SERPINA7 protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 12.5 ng/mL-200 ng/mL and the sensitivity is 12.5 ng/mL.

Customer Reviews and Q&A

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 Q&A
Q:

I need a TBG ELISA that would work for recombinant TBG in cell culture supernatant. Is this kit suitable for that?

A:
Thanks for your inquiry.
CSB-E09501H
The standard in this kit is CHO-derived recombinant protein. As we are not clear about the expression system and modifications of recombinant TBG, sorry we are not sure if it can be detected out or not. For this case we can only suggest you do a pretest first. We validated natural samples before and they can be detected out.

Target Background

Function
(From Uniprot)
Major thyroid hormone transport protein in serum.
Gene References into Functions
  1. A new SERPINA7 variant associated with thyroxine-binding globulin deficiency in three siblings. PMID: 29733970
  2. THBG is a potential plasma biomarker for COPD. PMID: 28579773
  3. mutation in a liver-specific enhancer region of the TBG gene caused inherited TBG deficiency. To our knowledge, the present study is the first report of an inherited endocrine disorder caused by a mutation in an enhancer region. PMID: 25361180
  4. TBG allosteric regulation is entropy driven. The presence of multiple S states may allow more efficient T4 release due to protease activity. PMID: 23458682
  5. the TBG promoter has a role in sustaining transgene expression in liver-specific pattern PMID: 22820390
  6. Allosteric modulation of hormone release from thyroxine and corticosteroid-binding globulins. PMID: 21325280
  7. Freshly isolated TBG-Chicago exists in loop expelled conformation. At 37C, the protein readily converts to a more stable loop inserted conformation with enhanced heat stability. PMID: 20429632
  8. an intragenic A/G polymorphism (125 bp upstream from exon 2) was identified. complete TBG deficiency was homozygous for the polymorphic TBG allele. PMID: 11889160
  9. Two novel variants in the thyroxine-binding globulin gene behind the diagnosis of TBG deficiency. homozygous. One involved codon 23 (TCA-->TAA) and the other, codon 223. PMID: 11916615
  10. Loop variants of the serpin thyroxine-binding globulin: implications for hormone release upon limited proteolysis. PMID: 11931635
  11. guanine deletion at position 1711, codon 201 (Asp) in exon 2 (GAC --> AC) led to a frame shift and premature termination at codon 206, causing a short TBG protein of 205 amino acids (AA) compared to 395 AA of the normal TBG. PMID: 17887925
  12. new serpina7 gene variant in three members of the same family results in the replacement of the normal asparagine 233 by isoleucine and, subsequently, in disruption of a glycosylation site PMID: 19415532
  13. The first exon (exon 0) is a short noncoding sequence located 1.62 kilobase pairs (kbp) upstream from exon 1. HNF-1 site (located 65 bp upstream of the TSS) is required for the hepatocyte specific expression of the hTBG gene. PMID: 8232304

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Subcellular Location
Secreted.
Protein Families
Serpin family
Tissue Specificity
Expressed by the liver and secreted in plasma.
Database Links

HGNC: 11583

OMIM: 300932

KEGG: hsa:6906

STRING: 9606.ENSP00000329374

UniGene: Hs.76838

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