Human Troponin I, slow skeletal muscle(TNNI1) ELISA kit

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Target Background

Name: Human Troponin I, slow skeletal muscle(TNNI1) ELISA kit
Brand: CUSABIO
SKU: CSB-EL024011HU

Product Details

Target Name

troponin I type 1 (skeletal, slow)

Alternative Names

2700018B22Rik ELISA Kit; AI747285 ELISA Kit; DKFZp451O223 ELISA Kit; si:dz182n13.3 ELISA Kit; slow skeletal muscle ELISA Kit; slow-twitch isoform ELISA Kit; SSTNI ELISA Kit; TNN1 ELISA Kit; Tnni1 ELISA Kit; TNNI1_HUMAN ELISA Kit; Troponin I ELISA Kit; Troponin I slow skeletal muscle ELISA Kit; Troponin I slow twitch isoform ELISA Kit; troponin I type 1 (skeletal; slow) ELISA Kit; Troponin I; slow isoform ELISA Kit

Abbreviation

TNNI1

Uniprot No.

P19237

Species

Homo sapiens (Human)

Sample Types

serum, plasma

Detection Range

0.312 ng/mL-20 ng/mL

Sensitivity

0.078 ng/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Signal Transduction

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human TNNI1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	91
	Range %	86-95
1:2	Average %	102
	Range %	97-107
1:4	Average %	91
	Range %	85-97
1:8	Average %	97
	Range %	91-103

Recovery

The recovery of human TNNI1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-98
EDTA plasma (n=4)	94	90-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
20	2.743	2.768	2.756	2.615
10	1.919	1.909	1.914	1.773
5	1.056	1.087	1.072	0.931
2.5	0.584	0.580	0.582	0.441
1.25	0.357	0.387	0.372	0.231
0.625	0.283	0.299	0.291	0.150
0.312	0.194	0.198	0.196	0.055
0	0.142	0.140	0.141

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx

Description

This Human TNNI1 ELISA Kit was designed for the quantitative measurement of Human TNNI1 protein in serum, plasma. It is a Sandwich ELISA kit, its detection range is 0.312 ng/mL-20 ng/mL and the sensitivity is 0.078 ng/mL.

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■ Q&A

HOW MANY DETERMINATIONS CAN BE PERFORMED WITH THE 24T ELISA KIT FOR TROPONIN I

Thanks for your inquiry!
CSB-EL024011HU
The standard is S0-S7. You can test 16 samples if you run single well.

Target Background

Function
(From Uniprot)

Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.

Gene References into Functions

Ultrasensitive troponin I was detectable in almost all individuals of a study sample reflecting middle-aged to elderly European general population. Ultrasensitive troponin concentrations exhibit an independent, graded, positive relation with incident CHD [coronary heart disease]. PMID: 28585121
Measuring mean platelet volume in conjunction with troponin I levels improves the diagnostic value of troponin I in acute coronary syndrome. PMID: 27524214
CT-1 was found to be associated with Tn-I, which is used to detect myocardial damage after OPCAB surgery. CT-1 may also be used to detect myocardial damage. PMID: 26334851
TNNI3K is a cardiac-specific gene, encoding a cardiac troponin I-interacting MAP kinase. In mice, Tnni3k is shown to play an important role in the regulation of cardiac differentiation and cardiac contractility PMID: 25791106
sTnI as a sensitive marker of skeletal muscle injury. PMID: 22323298
Elevations of troponin I and N-terminal pro-B-type natriuretic peptide (NT-proBNP) are common in patients with atrial fibrillation and independently related to increased risks of stroke and mortality. PMID: 22374183
Elevated post-operative troponin 1 levels predict long-term mortality in older patients undergoing emergency orthpedic surgery. PMID: 19811558
Evaluated the prevalence of TNN13 gene mutations in sudden death caused by cardiomyopathy (CM); in dilated CM cases, a new missense mutation Pro16Thr was detected. A single nucleotide polymorphism at -8 position of intron 3 was identified. PMID: 20817590
troponin I phosphorylation has a role in protein kinase C-mediated enhanced contractile performance of myocytes PMID: 12815045
MEF2 activation of the troponin I slow enhancer is repressed by hMusTRD1alpha1 PMID: 12857748
Low expression of ssTnI was found in 60% of patients in sinus rhythm or paroxysmal AF and in 8% of patients with chronic AF. Relative ssTnI mRNA expression was significantly lower in patients with AF than in those in sinus rhythm. PMID: 15289369
A biological marker of myocardial injury, but may also be increased after a grand mal seizure. PMID: 15367881
These results suggest that this R21C mutation in TnI affects the Ca(2+)-sensitizing effect of Tn, the ability of TnI to be readily phosphorylated by PKA and the stability of TnI to calpain. PMID: 16005017
Relationship between myocardial injury, assessed by cardiac troponin I (cTnI) levels, and outcome in selected critically ill patients without acute coronary syndromes or cardiac dysfunction PMID: 16236952
This marker in paatients with heart failure and anemia identifies patients at particularly high risk mortality. PMID: 16338262
Increased serum levels of troponin I is associated with calcinosis and coronary Artery Stenosis PMID: 16358990
The plasma levels of CRP and troponin I were also significantly higher in this group of nine patients, and correlated with urinary protein excretion. PMID: 16916777
A revised method of troponin exchange in pemeabilized cardiac trabeculae using vanadate: functional consequences of a HCM-causing mutation in troponin I. PMID: 17051347
a localized N-terminal region of TnI comprised of amino acids 33-80, which interacts with C-terminal regions of cTnC and cTnT, is of particular significance in transducing signaling of thin filament activation by strong cross-bridges PMID: 17099250

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Protein Families

Troponin I family

Tissue Specificity

Highest levels observed in human skeletal muscle (e.g. gastrocnemious muscle), differentiated cultures of primary human muscle cells and rhabdomyosarcoma cells cultured in low serum medium. Expressed in C2 muscle cell myoblasts and myotubes.

Database Links

HGNC: 11945

OMIM: 191042

KEGG: hsa:7135

STRING: 9606.ENSP00000337022

UniGene: Hs.320890

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Code	CSB-EL024011HU
Size	96T,5×96T,10×96T
Price	Request a Quote or Start an on-line Chat
Trial Size	24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.