Human Troponin I, slow skeletal muscle(TNNI1) ELISA kit

Instructions
Code CSB-EL024011HU
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name troponin I type 1 (skeletal, slow)
Alternative Names 2700018B22Rik ELISA Kit; AI747285 ELISA Kit; DKFZp451O223 ELISA Kit; si:dz182n13.3 ELISA Kit; slow skeletal muscle ELISA Kit; slow-twitch isoform ELISA Kit; SSTNI ELISA Kit; TNN1 ELISA Kit; Tnni1 ELISA Kit; TNNI1_HUMAN ELISA Kit; Troponin I ELISA Kit; Troponin I slow skeletal muscle ELISA Kit; Troponin I slow twitch isoform ELISA Kit; troponin I type 1 (skeletal; slow) ELISA Kit; Troponin I; slow isoform ELISA Kit
Abbreviation TNNI1
Uniprot No. P19237
Species Homo sapiens (Human)
Sample Types serum, plasma
Detection Range 0.312 ng/mL-20 ng/mL
Sensitivity 0.078 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human TNNI1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %91
Range %86-95
1:2Average %102
Range %97-107
1:4Average %91
Range %85-97
1:8Average %97
Range %91-103
Recovery
The recovery of human TNNI1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9589-98
EDTA plasma (n=4)9490-98
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
202.743 2.768 2.756 2.615
101.919 1.909 1.914 1.773
51.056 1.087 1.072 0.931
2.50.584 0.580 0.582 0.441
1.250.357 0.387 0.372 0.231
0.6250.283 0.299 0.291 0.150
0.3120.194 0.198 0.196 0.055
00.142 0.140 0.141
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days

Q&A and Customer Reviews

 Q&A
Q:


HOW MANY DETERMINATIONS CAN BE PERFORMED WITH THE 24T ELISA KIT FOR TROPONIN I

A:
Thanks for your inquiry!
CSB-EL024011HU
The standard is S0-S7. You can test 16 samples if you run single well.
 Customer Reviews

Target Data

Function Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.
Gene References into Functions
  1. Ultrasensitive troponin I was detectable in almost all individuals of a study sample reflecting middle-aged to elderly European general population. Ultrasensitive troponin concentrations exhibit an independent, graded, positive relation with incident CHD [coronary heart disease]. PMID: 28585121
  2. Measuring mean platelet volume in conjunction with troponin I levels improves the diagnostic value of troponin I in acute coronary syndrome. PMID: 27524214
  3. CT-1 was found to be associated with Tn-I, which is used to detect myocardial damage after OPCAB surgery. CT-1 may also be used to detect myocardial damage. PMID: 26334851
  4. TNNI3K is a cardiac-specific gene, encoding a cardiac troponin I-interacting MAP kinase. In mice, Tnni3k is shown to play an important role in the regulation of cardiac differentiation and cardiac contractility PMID: 25791106
  5. sTnI as a sensitive marker of skeletal muscle injury. PMID: 22323298
  6. Elevations of troponin I and N-terminal pro-B-type natriuretic peptide (NT-proBNP) are common in patients with atrial fibrillation and independently related to increased risks of stroke and mortality. PMID: 22374183
  7. Elevated post-operative troponin 1 levels predict long-term mortality in older patients undergoing emergency orthpedic surgery. PMID: 19811558
  8. Evaluated the prevalence of TNN13 gene mutations in sudden death caused by cardiomyopathy (CM); in dilated CM cases, a new missense mutation Pro16Thr was detected. A single nucleotide polymorphism at -8 position of intron 3 was identified. PMID: 20817590
  9. troponin I phosphorylation has a role in protein kinase C-mediated enhanced contractile performance of myocytes PMID: 12815045
  10. MEF2 activation of the troponin I slow enhancer is repressed by hMusTRD1alpha1 PMID: 12857748
  11. Low expression of ssTnI was found in 60% of patients in sinus rhythm or paroxysmal AF and in 8% of patients with chronic AF. Relative ssTnI mRNA expression was significantly lower in patients with AF than in those in sinus rhythm. PMID: 15289369
  12. A biological marker of myocardial injury, but may also be increased after a grand mal seizure. PMID: 15367881
  13. These results suggest that this R21C mutation in TnI affects the Ca(2+)-sensitizing effect of Tn, the ability of TnI to be readily phosphorylated by PKA and the stability of TnI to calpain. PMID: 16005017
  14. Relationship between myocardial injury, assessed by cardiac troponin I (cTnI) levels, and outcome in selected critically ill patients without acute coronary syndromes or cardiac dysfunction PMID: 16236952
  15. This marker in paatients with heart failure and anemia identifies patients at particularly high risk mortality. PMID: 16338262
  16. Increased serum levels of troponin I is associated with calcinosis and coronary Artery Stenosis PMID: 16358990
  17. The plasma levels of CRP and troponin I were also significantly higher in this group of nine patients, and correlated with urinary protein excretion. PMID: 16916777
  18. A revised method of troponin exchange in pemeabilized cardiac trabeculae using vanadate: functional consequences of a HCM-causing mutation in troponin I. PMID: 17051347
  19. a localized N-terminal region of TnI comprised of amino acids 33-80, which interacts with C-terminal regions of cTnC and cTnT, is of particular significance in transducing signaling of thin filament activation by strong cross-bridges PMID: 17099250

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Protein Families Troponin I family
Tissue Specificity Highest levels observed in human skeletal muscle (e.g. gastrocnemious muscle), differentiated cultures of primary human muscle cells and rhabdomyosarcoma cells cultured in low serum medium. Expressed in C2 muscle cell myoblasts and myotubes.
Database Links

HGNC: 11945

OMIM: 191042

KEGG: hsa:7135

STRING: 9606.ENSP00000337022

UniGene: Hs.320890

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