Human Urocortin-2(UCN2) ELISA kit

Instructions
Code CSB-EL025552HU
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name urocortin 2
Alternative Names SRP ELISA Kit; Stresscopin related peptide ELISA Kit; Stresscopin-related peptide ELISA Kit; Ucn II ELISA Kit; Ucn2 ELISA Kit; UCN2_HUMAN ELISA Kit; UCNI ELISA Kit; UCNII ELISA Kit; UR ELISA Kit; Urocortin II ELISA Kit; Urocortin related peptide ELISA Kit; Urocortin-2 ELISA Kit; Urocortin-related peptide ELISA Kit; URP ELISA Kit
Abbreviation UCN2
Uniprot No. Q96RP3
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates, cell culture supernates
Detection Range 6.25 pg/mL-400 pg/mL
Sensitivity 1.56 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cardiovascular
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human UCN2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 84  
Range % 80-88  
1:2 Average % 90  
Range % 85-95  
1:4 Average % 97  
Range % 92-110  
1:8 Average % 93  
Range % 86-98  
Recovery
The recovery of human UCN2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 95 89-99  
EDTA plasma (n=4) 104 100-110  
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
400 2.258 2.302 2.280 2.142  
200 1.735 1.696 1.716 1.578  
100 1.126 1.101 1.114 0.976  
50 0.678 0.637 0.658 0.520  
25 0.412 0.434 0.423 0.285  
12.5 0.311 0.309 0.310 0.172  
6.25 0.243 0.254 0.249 0.111  
0 0.137 0.139 0.138    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days

Target Data

Function Suppresses food intake, delays gastric emptying and decreases heat-induced edema. Might represent an endogenous ligand for maintaining homeostasis after stress.
Gene References into Functions
  1. laryngeal cancer cells were enriched in the recurrent chimera COL7A1-UCN2, which potentially affected cancer stem cell transition PMID: 29499655
  2. examination of the local and systemic cardiovascular effects of urocortin 2 and urocortin 3 in healthy subjects and patients with heart failure PMID: 27275843
  3. Data show that corticotropin releasing hormone receptor 2 (Crhr2) protein is abundantly expressed in the heart, and plasma Ucn2 levels were higher in patients with heart failure compared to healthy controls, indicating that endogenous Crhr2 is associated with heart failure. PMID: 28550160
  4. up-regulated in placenta, fetal membranes, and myometrium in labor PMID: 25426872
  5. Corticotrophin-Releasing Factor (CRF) and the urocortins are potent regulators of the inflammatory phenotype of human and mouse white adipocytes. PMID: 24835211
  6. Stresscopin-related peptide induces a weaker enhancement of cardiovascular function through corticotropin-releasing factor receptorn 2 than that induced by stresscopin. PMID: 23850799
  7. Solubilized SUMO-eXact-preproUCN2 was used successfully to generate two high affinity mouse monoclonal antibodies specific to the pro-region of urocortin 2. PMID: 24291344
  8. UCN2 is significantly associated with AAA and inhibits VSMC proliferation by inducing a G1 cell cycle arrest suggesting a plausible regulatory role in AAA pathogenesis PMID: 24107226
  9. UCNII caused cell death via different membrane-disrupting mechanisms that involve aggregation & membrane depolarization in bacteria and pore formation in Leishmania even inside macrophages. Innate immune cells produce UCNII in response to infections. PMID: 24249730
  10. Description of ucn2 posttranslational processing and peptide sequence. Hypoxia and glycosylation, paradigms that might influence secretion or processing of gene products, did not significantly impact hUcn 2 prohormone cleavage. PMID: 23493376
  11. study showed that Ucn2 and Ucn3 differentially regulate the LPS-induced TNF-alpha and IL-10 expression and secretion in trophoblast explants acting through CRH-R2. Ucn2 is proinflammatory. PMID: 22000474
  12. These data suggest a possible involvement of urocortin 2 and Ucn 3 in the mechanisms of endometriosis. PMID: 21454316
  13. Confirm the anti-inflammatory function of VIP, through the modulation of the expression of CRF system that impacts in a reduction of mediators with inflammatory/destructive functions. PMID: 21360527
  14. Unlike urocortin-1 levels, higher levels of urocortin 2 are not associated with worse left ventricular diastolic performance in patients with chronic systolic heart failure. PMID: 20670842
  15. Urocortin 2 may play a role in the regulation of estradiol production throughout pregnancy, thereby contributing to the placental regulation of key reproductive events in pregnancy maintenance and parturition. PMID: 19417223
  16. Placental Ucn2 and Ucn3 expression is sensitive to O(2) tensions and mediated by HIF-1alpha. In preeclampsia, the increased expression of both peptides may reflect a response to the oxidative stress. PMID: 20616367
  17. SCP and UCN are potent activators of the p42/44 MAPK pathway, with SRP able to induce phosphorylation of p42/44 MAPK as well, albeit not as pronounced PMID: 14519439
  18. data suggest a distinctive role for corticotropin-releasing hormone receptor 2 specific agonists like urocortin II in the control of myometrial contractility during human pregnancy PMID: 14592950
  19. data indicate that intracisternal Ucn 2 induced a central CRF(2)-mediated inhibition of gastric emptying involving sympathetic alpha(1)-adrenergic mechanisms independent from the vagus contrasting with the vagal-dependent inhibitory actions of CRF & Ucn 1 PMID: 16223946
  20. the 38 amino acid urocortin 2 peptide is the minimal pharmacophore PMID: 16476507
  21. UCN2 and UCN3 are expressed in human placenta, decidua, and fetal membranes and may be proposed as regulators of placental vascular endothelial tone. PMID: 16626608
  22. Both peptides were immunolocalized in epithelial, stromal, and endothelial cells. PMID: 17074339

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Subcellular Location Secreted
Protein Families Sauvagine/corticotropin-releasing factor/urotensin I family
Database Links

HGNC: 18414

OMIM: 605902

KEGG: hsa:90226

STRING: 9606.ENSP00000273610

UniGene: Hs.631914

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