Human Vascuar endothelial cell growth factor receptor 3,VEGFR-3/Flt-4 ELISA Kit

Code CSB-E04765h
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Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P35916
Lead Time 3-5 working days
Abbreviation FLT4
Target Name fms-related tyrosine kinase 4
Alias FLT41, LMPH1A, PCL, VEGFR3, soluble VEGFR3 variant 1|soluble VEGFR3 variant 2|soluble VEGFR3 variant 3|vascular endothelial growth factor receptor 3
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.156 ng/mL-10 ng/mL
Sensitivity 0.039 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details This gene encodes a tyrosine kinase receptor for vascular endothelial growth factors C and D. The protein is thought to be involved in lymphangiogenesis and maintenance of the lymphatic endothelium. Mutations in this gene cause hereditary lymphedema type IA.
HGNC 12682
RGD 619800
MGI 109124
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human VEGFR-3/Flt-44 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 98
Range % 91-105
1:2 Average % 90
Range % 85-96
1:4 Average % 95
Range % 85-105
1:8 Average % 92
Range % 83-98
Recovery
The recovery of human VEGFR-3/Flt-44 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 95 86-100
EDTA plasma (n=4) 93 87-98
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected
10 2.549 2.516 2.533 2.420
5 1.517 1.557 1.537 1.424
2.5 0.796 0.758 0.777 0.664
1.25 0.472 0.489 0.481 0.368
0.625 0.302 0.315 0.309 0.196
0.312 0.259 0.246 0.253 0.140
0.156 0.164 0.171 0.168 0.055
0 0.111 0.115 0.113  
References
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Function Tyrosine-protein kinase that acts as a cell-surface receptor for VEGFC and VEGFD, and plays an essential role in adult lymphangiogenesis and in the development of the vascular network and the cardiovascular system during embryonic development. Promotes proliferation, survival and migration of endothelial cells, and regulates angiogenic sprouting. Signaling by activated FLT4 leads to enhanced production of VEGFC, and to a lesser degree VEGFA, thereby creating a positive feedback loop that enhances FLT4 signaling. Modulates KDR signaling by forming heterodimers. The secreted isoform 3 may function as a decoy receptor for VEGFC and/or VEGFD and play an important role as a negative regulator of VEGFC-mediated lymphangiogenesis and angiogenesis. Binding of vascular growth factors to isoform 1 or isoform 2 leads to the activation of several signaling cascades; isoform 2 seems to be less efficient in signal transduction, because it has a truncated C-terminus and therefore lacks several phosphorylation sites. Mediates activation of the MAPK1/ERK2, MAPK3/ERK1 signaling pathway, of MAPK8 and the JUN signaling pathway, and of the AKT1 signaling pathway. Phosphorylates SHC1. Mediates phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase. Promotes phosphorylation of MAPK8 at 'Thr-183' and 'Tyr-185', and of AKT1 at 'Ser-473'.
Involvement in disease Lymphedema, hereditary, 1A (LMPH1A); Hemangioma, capillary infantile (HCI)
Subcellular Location Cell membrane, Single-pass type I membrane protein, Cytoplasm, Nucleus, Note=Ligand-mediated autophosphorylation leads to rapid internalization, SUBCELLULAR LOCATION: Isoform 1: Cell membrane, Single-pass type I membrane protein
Protein Families Protein kinase superfamily, Tyr protein kinase family, CSF-1/PDGF receptor subfamily
Tissue Specificity Detected in endothelial cells (at protein level). Widely expressed. Detected in fetal spleen, lung and brain. Detected in adult liver, muscle, thymus, placenta, lung, testis, ovary, prostate, heart, and kidney.
Database Links

HGNC: 3767

OMIM: 136352

KEGG: hsa:2324

STRING: 9606.ENSP00000261937

UniGene: Hs.646917

Pathway MAPK signaling pathway
PI3K-Akt signaling pathway
Focal adhesion
Ras signaling pathway
Rap1 signaling pathway

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