Human Xaa-Pro Dipeptidase/Prolidase(PEPD) ELISA Kit

Code CSB-E16196h
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Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P12955
Lead Time 3-5 working days
Abbreviation PEPD
Protein Biological Process 1 Developmental Protein
Target Name peptidase D
Alias MGC10905, PROLIDASE, aminoacyl-L-proline hydrolase|imidodipeptidase|prolidase|proline dipeptidase
Species Homo sapiens (Human)
Protein Biological Process 3 Collagen degradation
Sample Types serum, plasma, tissue homogenates
Detection Range 93.75 mU/mL-6000 mU/mL
Sensitivity 23.4 mU/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cell Biology
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
HGNC 8840
RGD 1594571
MGI 97542
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human PEPD in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:5Average %84
Range %80-89
1:10Average %88
Range %84-92
1:20Average %90
Range %85-96
1:40Average %85
Range %80-92
Recovery
The recovery of human PEPD spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9691-103
EDTA plasma (n=4)9288-98
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
mU/mlOD1OD2AverageCorrected
60002.652 2.472 2.562 2.413
30002.087 1.976 2.032 1.883
15001.293 1.204 1.249 1.100
7500.598 0.561 0.580 0.431
3750.379 0.357 0.368 0.219
187.50.208 0.214 0.211 0.062
93.750.165 0.159 0.162 0.013
00.153 0.145 0.149  
References
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Function Splits dipeptides with a prolyl or hydroxyprolyl residue in the C-terminal position. Plays an important role in collagen metabolism because the high level of iminoacids in collagen.
Involvement in disease Prolidase deficiency (PD)
Protein Families Peptidase M24B family, Eukaryotic-type prolidase subfamily
Database Links

HGNC: 8840

OMIM: 170100

KEGG: hsa:5184

STRING: 9606.ENSP00000244137

UniGene: Hs.36473

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