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Product Name:

Human alpha II-spectrin breakdown product SBDP145 ELISA kit

Product Type : ELISA Kit
Code : CSB-EQ028022HU
Size : 96T,5×96T,10×96T
Abbreviation : SBDP145
Species : Human
Sample Types : serum, plasma, tissue homogenates, cerebrospinal fluid (CSF)
Detect Range : 0.625 ng/ml-40 ng/ml
Sensitivity : 0.156 ng/ml.
Research Area : Others
Protocol :
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Precision :
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity :
To assess the linearity of the assay, samples were spiked with high concentrations of human SBDP145 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %85
Range %80-92
1:2Average %100
Range %95-105
1:4Average %90
Range %85-96
1:8Average %105
Range %100-109
Recovery :
The recovery of human SBDP145 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 8785-90
EDTA plasma (n=4)9085-96
Typical Data :
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
402.552 2.678 2.615 2.447
202.049 2.086 2.068 1.900
101.458 1.469 1.464 1.296
50.912 0.954 0.933 0.765
2.50.636 0.678 0.657 0.489
1.250.442 0.468 0.455 0.287
0.6250.341 0.351 0.346 0.178
00.167 0.168 0.168  
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