Human alpha II-spectrin breakdown product SBDP145 ELISA kit

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Human alpha II-spectrin breakdown product SBDP145 ELISA kit

Citations (2) Protocol MSDS

Code	CSB-EQ028022HU
Size	96T,5×96T,10×96T
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Product Details
Citations (2)
Customer Reviews and Q&A

Target Name

alpha II-spectrin breakdown product SBDP145

Abbreviation

SBDP145

Species

Homo sapiens (Human)

Sample Types

serum, plasma, tissue homogenates, cerebrospinal fluid (CSF)

Detection Range

0.625 ng/mL-40 ng/mL

Sensitivity

0.156 ng/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Others

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human SBDP145 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	85
	Range %	80-92
1:2	Average %	100
	Range %	95-105
1:4	Average %	90
	Range %	85-96
1:8	Average %	105
	Range %	100-109

Recovery

The recovery of human SBDP145 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	87	85-90
EDTA plasma (n=4)	90	85-96

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
40	2.552	2.678	2.615	2.447
20	2.049	2.086	2.068	1.900
10	1.458	1.469	1.464	1.296
5	0.912	0.954	0.933	0.765
2.5	0.636	0.678	0.657	0.489
1.25	0.442	0.468	0.455	0.287
0.625	0.341	0.351	0.346	0.178
0	0.167	0.168	0.168

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.

Description

This Human SBDP145 ELISA Kit was designed for the quantitative measurement of Human SBDP145 protein in serum, plasma, tissue homogenates, cerebrospinal fluid (CSF). It is a Sandwich ELISA kit, its detection range is 0.625 ng/mL-40 ng/mL and the sensitivity is 0.156 ng/mL .

Calpain inhibition as a novel therapeutic strategy for aortic dissection with acute lower extremity ischemia Q Tan, X Wang, W Xu, K Song, Y Xiong, Z Jiang, J Li,Molecular Medicine,2025
Calpain‐2‐Mediated Endothelial Focal Adhesion Disruption in Thoracic Aortic Dissection X Teng, Y Wang, H Huang, Y Ding, J Wang,Advanced Science,2025

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

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