Human anti-cyclic citrullinated peptide antibody (anti-CCP antibody) ELISA Kit

Instructions
Code CSB-E09077h
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Description

This Human anti-cyclic citrullinated peptide antibody (anti-CCP antibody, ACCPA) ELISA Kit is designed to qualitatively detect the anti-CCP antibody in the serum. It employs the qualitative enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with CCP antigen. Samples are pipetted into the wells with anti-human IgG conjugated HRP. Any antibodies specific for CCP present in the samples will bind to the pre-coated CCP antigen. Following a wash to remove any unbound reagent, the TMB substrate solution is added to the wells and color develops in proportion to the amount of human anti-CCP antibody bound in the initial step. The color development is stopped and the intensity of the color is measured by a microplate reader at 450 nm.

This assay has high sensitivity and excellent specificity for detection of human anti-CCP antibody. And it also has been validated with precision less than 15% and lot-to-lot consistency. Get more details from the product instructions.

ACCPA is a type of autoantibodies against citrullinated peptides and proteins. They target healthy tissues in the joints. And they are detected in the majority of patients suffering from rheumatoid arthritis (RA). An ACCPA test is helpful for the diagnosis of RA, along with or after a rheumatoid factor (RF) test. ACCPA is predictive of the progression of patients, indicating more erosive disease or increase joint involvement.

Target Name anti-cyclic citrullinated peptide antibody (anti-CCP antibody)
Abbreviation ACCPA
Species Homo sapiens (Human)
Sample Types serum
Detection Range Request Information
Sensitivity Request Information
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Autoimmunity
Precision
Intra-assay Precision (Precision within an assay): CV%<15%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<15%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Typical Data
             
             
Test parameter specification test result  
Positive control >1 2.078  
Negative control <0.1 0.075  
Positive rate 10,Positive 100%  
Negative rate 10,Negative 100%  
Materials provided
  • A 96-well Assay plate --The 96-well plate has been pre-coated with CCP antigen.
  • Negative Control (1 x 0.8 ml)
  • Positive Control (1 x 0.8 ml)
  • HRP-conjugated IgG antibody(1 x 10m)l --Bind to the human anti-CCP antibody, and HRP catalyzes the TMB to elicit a chromogenic reaction.
  • Sample Diluent (2 x 20 ml) --Dilute the sample to an appropriate concentration.
  • Wash Buffer (25x concentrate) (1 x 20 ml) --Wash away unbound or free substances.
  • Substrate A (1 x 5 ml) --Mix with substrate B and interact with HRP, eliciting a Chromogenic reaction.
  • Substrate B (1 x 5 ml) --Mix with substrate A and interact with HRP, eliciting a Chromogenic reaction.
  • Stop Solution (1 x 5ml) --Stop the color reaction. The solution color immediately turns from blue to yellow.
  • Four Adhesive Strips (For 96 wells)
  • An Instruction manual
Materials not provided
  • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 600 nm - 630 nm.
  • An incubator that can provide stable incubation conditions up to 37°C±5°C.
  • Centrifuge
  • Vortex
  • Squirt bottle, manifold dispenser, or automated microplate washer
  • Absorbent paper for blotting the microtiter plate
  • 50-300ul multi-channel micropipette
  • Pipette tips
  • Single-channel micropipette with different ranges
  • 100ml and 500ml graduated cylinders
  • Deionized or distilled water
  • Timer
  • Test tubes for dilution
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Customer Reviews and Q&A

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 Q&A
Q:

I would like to order Human ELISA kit for anti-CCP3.

A:
Thanks for your inquiry.
Regarding your request,we have this kit for you:#CSB-E09077h,Human anti-cyclic citrullinated peptide antibody (anti-CCP antibody) ELISA Kit. I will ask our distributor in your area to send you a quote by email.
Q:

I found out that that it was a qualitative kit. Is there any way for me to obtain the concentration of the positive control?
I can only get the volume from the kit and not the concentration. Would like to have the concentration so that i can use it for quantitative analysis later on.

A:
Thanks for your inquiry.
Yes this kit is for qualitative determination.Thus you can not obtain the concentration.It can not be used for quantitative analysis.

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