Human carcinoembryonic antigen,CEA ELISA Kit

Instructions
Code CSB-E04767h
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name carcinoembryonic antigen-related cell adhesion molecule 5
Alternative Names Carcinoembryonic antigen ELISA Kit; Carcinoembryonic antigen-related cell adhesion molecule 5 ELISA Kit; CD66e ELISA Kit; CEA ELISA Kit; Ceacam5 ELISA Kit; CEAM5_HUMAN ELISA Kit; DKFZp781M2392 ELISA Kit; Meconium antigen 100 ELISA Kit; OTTHUMP00000199032 ELISA Kit; OTTHUMP00000199033 ELISA Kit; OTTHUMP00000199034 ELISA Kit
Abbreviation CEACAM5
Uniprot No. P06731
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 5 ng/mL-120 ng/mL
Sensitivity 2 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cancer
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<10%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<15%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human CEA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 98  
Range % 92-106  
1:2 Average % 95  
Range % 91-99  
1:4 Average % 104  
Range % 98-114  
1:8 Average % 85  
Range % 80-89  
Recovery
The recovery of human CEA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 92 88-98  
EDTA plasma (n=4) 102 98-108  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected  
120 1.935 2.042 1.989 1.951  
60 1.318 1.428 1.373 1.335  
30 0.844 0.864 0.854 0.816  
10 0.388 0.376 0.382 0.344  
5 0.224 0.227 0.226 0.188  
0 0.037 0.038 0.038    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Q&A and Customer Reviews

 Q&A
Q:

Could you please help me with your technical team - are the antibodies used in this ELISA kit polyclonal antibodies or monoclonal antibodies?

A:
Thanks for your inquiry.
#CSB-E04767h,Human carcinoembryonic antigen,CEA ELISA Kit
Capture antibody:mouse monoclonal antibody;Detection antibody:mouse monoclonal antibody.

Target Data

Function Cell surface glycoprotein that plays a role in cell adhesion and in intracellular signaling. Receptor for E.coli Dr adhesins.
Gene References into Functions
  1. Positive CEA mRNA in low rectal cancer is a factor that predisposes patients to a high risk for overall recurrence, especially for local recurrence. PMID: 28291565
  2. High carcinoembryonic antigen elevation is associated with recurrence in rectal cancer. PMID: 29774483
  3. CEA and CA19-9 are cancer antigens that are late markers of carcinogenesis, with significantly elevated serum concentrations in case of colon cancer with already developed metastases. Older age group of patient has significantly elevated levels of both antigens. Cancer was twice more common in men than in women. PMID: 25568506
  4. High CEA expression is associated with breast cancer metastasis. PMID: 29433529
  5. Pretreatment serum CEA levels over 30.02 ng/mL on behalf of worse characteristics and unfavourable tumor behavior, and a poor prognosis for a nearly doubled risk of mortality in gastric cancer patients. PMID: 29358864
  6. Elevation of CEA level was an independent risk factor for the poor prognosis of early gastric cancer. PMID: 29121872
  7. The elevation of serum CEA level was independent from the other tumor markers in hypohidrotic condition represented by acquired idiopathic generalized anhidrosis. PMID: 28295553
  8. Elevated CEA levels during targeted therapy may be a more sensitive predictor of explosive lung adenocarcinoma progression in patients harboring mutant EGFRs compared to traditional imaging methods. PMID: 28705152
  9. Increased CEA expression is associated with colorectal cancer. PMID: 28128739
  10. The detection of TK1 combined with that of cytokeratin-19 fragment (CYFRA21-1), CEA or NSE increased the diagnostic value of TK1 for lung squamous cell carcinoma, adenocarcinoma and small cell lung cancer, respectively. PMID: 29247745
  11. In patients with non-small-cell lung cancer treated with nivolumab, worse pretreatment performance status, and higher carcinoembryonic antigen were associated with inferior progression-free survival. PMID: 29277824
  12. To examine the relation of carcinoembryonic antigen (CEA) response with tumor response and survival in patients with (K)RAS wild-type metastatic colorectal cancer receiving first-line chemotherapy in the FIRE-3 trial comparing FOLFIRI plus cetuximab versus FOLFIRI plus bevacizumab PMID: 27234640
  13. This is the first population-based investigation of a large cohort of exclusively stage I rectal cancer patients providing compelling evidence that elevated preoperative CEA level is a strong predictor of worse overall and cancer-specific survival. PMID: 27067235
  14. Elevated levels of systemic IFNgamma and IL-6 implied that the CEACAM5-specific T cells had undergone immune activation in vivo but only in patients receiving high-intensity pre-conditioning. PMID: 28660319
  15. Combination of the two tumor markers, CEA and CYFRA21-1, is a predictive and prognostic marker of docetaxel monotherapy for previously treated NSCLC patients PMID: 28870944
  16. Patients with CEA-High stage I NSCLC have a higher risk of regional or systemic relapse and should be followed-up carefully. PMID: 28870949
  17. Postoperative CEA level >2.5 ng/ml is a predictor of distant metastasis and a negative prognostic factor for survival in rectal cancer patients who receive preoperative chemoradiotherapy and curative surgery. PMID: 27553616
  18. Data show that triple-negative breast cancer (TNBC) patients with pre-therapeutic serum high levels of both carcinoembryonic antigen (CEA) and cancer antigen 15-3 (CA15-3) had shorter overall survival (OS) and disease-free survival (DFS) rates than those in the low-level groups. PMID: 27561099
  19. Serum CEA level is not influenced by the presence of benign dominant biliary stricture or superimposed bacterial cholangitis in patients with primary sclerosing cholangitis. PMID: 27943017
  20. Preoperative-CEA was elevated (>/=5.0 ng/mL) in 73.6% of the colorectal cancer patients and remained elevated after surgery in 32.7% of the patients. Elevated postoperative-CA 19-9 (>/=50 U/mL) was observed in 9.5% of the patients. Neither elevated pre-CEA nor elevated pre-CA 19-9 was associated with relapse-free survival (RFS) but both elevated post-CEA and elevated post-CA 19-9 were associated with reduced RFS. PMID: 27664887
  21. the diagnostic sensitivity and specificity of serum reactive oxygen species modulator 1 were only 41.38% and 86.21%, respectively, with the cutoff value of 27.22 ng/mL. The sensitivity and specificity of pleural fluid carcinoembryonic antigen were 69.23% and 88.00%, respectively, at the cutoff value of 3.05 ng/mL, while serum carcinoembryonic antigen were 80.77% and 72.00% at the cutoff value of 2.60 ng/mL. PMID: 28459208
  22. Taken together, the results of our study identified CEACAM5 as a novel cell surface binding target of Middle East respiratory syndrome coronavirus that facilitates infection by augmenting the attachment of the virus to the host cell surface. PMID: 27489282
  23. Data show that carcinoembryonic antigen (CEA) modestly differentiated between mucinous and nonmucinous lesions, and amylase did not distinguish intraductal papillary mucinous neoplasms (IPMNs) from mucinous cystadenomas (MCAs). PMID: 26646270
  24. In this study, we detected the tumor marker carcinoembryonic antigen (CEA) using the QD-LFTS system, which allowed quantitative analysis in the range of 1-100ng/mL with an ideal detection limit of 0.049ng/mL. Thus, the system is suitable for detecting CEA in the clinically accepted range. We also detected 70 positive and 30 negative serum samples using the Handing system, which exhibited good specificity and sensitivity. PMID: 27825889
  25. As a proof-of-concept study, the constructed platform exhibits good specificity for CEA and the detection limit reaches as low as 8pg/mL (45 fM) with a wide linear range from 0.01 to 60ng/mL in the both cases PMID: 27886601
  26. Under the optimal conditions, the proposed immunosensor was used for the detection of CEA with wide dynamic range in the range from 5 fg/mL to 50ng/mL with a low detection limit of 2fg/mL (S/N=3). PMID: 27871047
  27. Relative CL intensity of all-in-one dual-aptasensor, operated with the competitive reaction of CEA and hemin in the presence of the dual aptamer, was exponentially decreased with the increase of CEA concentration in human serum PMID: 27875751
  28. Data indicate that serum tumor markers showed significant shorter 3-year progression-free survival (PFS) in higher levels compared to lower levels for S-CYFRA 21-1 (cytokeratin 19 fragment), S-SCCA and S-CEA. PMID: 26432331
  29. Multivariate logistic regression analysis revealed TC and DeltaCEA as independent factors in predicting TRG; TC showed a sensitivity of 62.79 %, a specificity of 91.49 %, a Youden index of 0.543, a cutoff value of 5.52, and an AUC of 0.800 compared with DeltaCEA (sensitivity 76.74 %, specificity 65.96 %, Youden index 0.427, and AUC 0.761). PMID: 26531721
  30. Data suggest that joint detection of receptor-binding cancer antigen expressed on SiSo cells (RCAS1) and carcinoembryonic antigen (CEA) can improve the diagnostic sensitivity and specificity. PMID: 26438059
  31. High CEA level is associated with Oral Squamous Cell Carcinoma. PMID: 27165212
  32. High CEA expression is associated with metastasis and recurrence in endometrial cancer. PMID: 26779635
  33. ZKSCAN3 appears to promote colorectal tumor progression and invasion. ZKSCAN3 may facilitate hepatic metastasis of CRC associated with CEA particularly in cases with CEA-producing tumor PMID: 27127149
  34. we evaluated the clinical performance of LOCItrade mark-based tumor marker assays CEA, CA19-9, CA15-3, CA125 and AFP in patients with gastrointestinal cancer and demonstrated their high diagnostic power PMID: 28011514
  35. High carcinoembryonic Antigen expression is associated with gastric cancer. PMID: 25124614
  36. High CEA expression is associated with Squamous cell carcinoma of the skin. PMID: 27039776
  37. Preparation of Au-polydopamine functionalized carbon encapsulated FeO magnetic nanocomposites and their application for ultrasensitive detection of carcino-embryonic antigen. PMID: 26868035
  38. Elevated Carcinoembryonic Antigen Levels are associated with Colon Cancer. PMID: 26759308
  39. Baseline serum CEA levels can serve as predictive factors for the treatment of EGFR-TKI in non-small cell lung cancer patients harboring EGFR mutations. PMID: 27072247
  40. BALF and serum NSE, CEA, and CYFRA21.1 elevated in lung cancer, which had prompt value for pathology, especially significant for BALF. PMID: 27072263
  41. Significant levels of CEA, CYFRA 21-1, NSE, and TSGF were detected in the serum. The amounts found were useful for diagnosing non-small cell lung cancer (NSCLC) patients who depended on the currently limited biomarker development. PMID: 27072222
  42. We conclude that patients with high preoperative serum CEA levels should have more intensive follow-up for detection of synchronous liver metastasis. PMID: 26756614
  43. Elevated CEA expression is associated with Gastric Cancer. PMID: 26620645
  44. CEA, NSE, CA125 and pro-GRP could serve as biomarkers for SCLC, and CEA and CYFRA21-1 could serve as biomarkers for NSCLC. Pro-GRP, CA125 and CEA were related to the clinical stages of lung cancer PMID: 26560853
  45. In this phase I/II study, 14 high-risk disease-free ovarian (OC) and breast cancer (BC) patients after completion of standard therapies were vaccinated with MUC1, ErbB2 and carcinoembryonic antigen (CEA) HLA-A2+-restricted peptides and Montanide. PMID: 26892612
  46. combined pre-chemoradiotherapy CEA and post-chemoradiotherapy CEA levels enable more accurate prediction of rectal adenocarcinoma prognosis PMID: 26962798
  47. ESCC patients with lower Cyfra21-1 and CEA, higher miR-7 and severe myelosuppression were much more sensitive to CRT. PMID: 26708917
  48. Cyst fluid CEA levels have a clinically suboptimal accuracy level in differentiating pancreatic mucinous cystic neoplasms from pancreatic nonmucinous cystic neoplasms. PMID: 26077458
  49. High carcinoembryonic antigen expression is associated with Colon Adenomas. PMID: 27100181
  50. Serum CYFRA21-1 and CEA can be used as prognostic factors of NSCLC patients. Combinative detection of the two indices will be more reliable. PMID: 26333429
  51. The multivariate stepwise logistic regression identified the following biomarkers as the best gastric cancer predictors: CEA, CA72-4, pepsinogen I, Helicobacter pylori presence and MMP7 PMID: 27069188
  52. show the upregulation of the tumor-associated antigens CEACAM5/6 by trans-signaling of the pro-inflammatory cytokine IL-6 PMID: 26673628
  53. Elevated D-dimer and INR values may indicate higher disease stage. Correlation of D-dimer levels with CEA supports their value for assessing tumor burden. PMID: 25792472
  54. Data indicate that the diagnostic efficiency of V-set domain containing T cell activation inhibitor 1 (sB7-H4) combined carcinoembryonic antigen (CEA) was superior to either sB7-H4 or CEA. PMID: 26301886
  55. Elevated serum carcinoembryonic antigen is associated with non-small cell lung cancer. PMID: 24889374
  56. Among patients with elevated concentrations of the CA 15-3, CEA, and TPA concordance could be observed between serial changes of the hypermethylated RASSF1A gene and the protein markers PMID: 26339655
  57. Preoperative serum levels of CEA and CA15-3 are independent prognostic parameters for breast cancer. PMID: 26207909
  58. This prospective study validates a previous study testing the detection of Colorectal Cancer based on TIMP-1 and CEA levels. PMID: 26254391
  59. Results suggest that lipid A of Gram-negative bacteria might play a role in preventing the release of CD66e (CEA) from mucosal surfaces and promote mucosal colonization by bacteria. PMID: 26208386
  60. Elevation of serum CEA is associated with Non-small Cell Lung Cancer. PMID: 26163610
  61. CEACAM5 expression was increased progressively from normal gastric mucosa to chronic atrophic gastritis, intestinal metaplasia, dysplasia and finally to gastric cancer. PMID: 26374829
  62. level predicts pathologic complete response in rectal cancer treatment PMID: 26559272
  63. The maternal serum levels of CA 125, CEA and CA 19-9 were increased during third trimester of pregnancy, but these elevations were within the normal range. PMID: 21792548
  64. High serum carcinoembryonic antigen levels are associated with gastric adenocarcinoma. PMID: 25921105
  65. The serum levels of carcinoembryonic antigen (CEA), nerve cell-specific enolase, and lactate dehydrogenase in 281 patients with small cell lung cancer (SCLC) were analyzed in this study. PMID: 25936590
  66. CEA response after 1 month of EGFR-TKI therapy could be a useful marker, worthy to further studies, as early predictor of treatment outcome in EGFR wild-type/unknown unselected NSCLC cases for which no molecular predictor is yet available PMID: 25731731
  67. Pre-treatment levels of CEA were not associated with any of the baseline characteristics (except primary tumour localisation) or to differences in PFS or OS PMID: 25608838
  68. Neither human CEACAM5 expression nor chronic smoke exposure nor a combination of both was sufficient as prerequisites for the establishment of chronic M. catarrhalis lung colonization. PMID: 26047639
  69. The CEACAM5 expression patterns were associated with tumor progression PMID: 25042385
  70. CEA and CA19-9, provide additional diagnostic information in gastrointestinal malignancies but only CA19-9 was significantly associated with peritoneal metastasis. PMID: 26026118
  71. Increase of serum CA 19-9 and carcinoembryonic antigen levels are associated with colorectal cancer. PMID: 25442815
  72. Elevated precystectomy serum levels of CEA is an independent predictor of worse oncological outcome in patients with invasive urothelial carcinoma of the bladder. PMID: 24680660
  73. LOX with CEA, CA724, CA199, and CA125 could increase the sensitivity of predicting lymph nodes metastasis and peritoneal metastasis in gastric cancer PMID: 25060181
  74. results indicate the correlation of the tumor markers CEA and CA 19-9 with radiological imaging in patients with metastatic colorectal cancer receiving first-line chemotherapy PMID: 25023402
  75. In the present meta-analysis, preoperative serum CEA overexpression indicates a poor prognosis for patients with non-small cell lung cancer PMID: 25023403
  76. exercise for 2-3 days per week decreases the expression of CEA and improves body condition. PMID: 24747263
  77. a new mechanistic insight into how the CEA IgV-like N domain participates in cellular events that can have a macroscopic impact in terms of cancer progression and metastasis. PMID: 24388361
  78. Overall survival of lung cancer patients was significantly longer in those with normal pre-treatment serum CEA levels than in those with abnormal pre-treatment serum CEA levels. PMID: 24440973
  79. The existence of novel CEACAM5 splice variants and splice variant-derived protein isoforms in gastrointestinal cancer cell lines, is reported. PMID: 24070190
  80. The elevation of serum CEA might be attributed to the presence of local inflammation in the lung. PMID: 24419710
  81. A direct correlation between the amount of cellular CEA protein and 5-FU resistance in CEA expressing cells. PMID: 24293105
  82. Results show that scFvs against HER-2/neu and carcinoembryonic antigen (CEA) were isolated. PMID: 23845693
  83. this study discovered that CEA expression by colorectal cancer cells influences the function,and the interactions between the E-cadherin junction complex proteins may explain the link between the elevated levels of CEA and the increase in soluble E-cadherin during the progression of colorectal cancer. PMID: 24726916
  84. A CEA level reduction is a sensitive and specific marker of OR, as well as a sensitive indicator for progression to chemotherapy in patients with advanced NSCLC. PMID: 23697613
  85. The combined detection of IL-17 and CEA may be more valuable in the differential diagnosis between malignant pleural effusions and benign pleural effusions. PMID: 24072496
  86. High carcinoembryonic antigen is associated with recurrence in breast cancer. PMID: 24065471
  87. This study identified several loci associated with CA19-9, CEA and AFP concentrations. The ABO variants were associated with risk of oesophageal squamous cell and pancreatic cancers and AFP variants with risk of hepatocellular cancer. PMID: 23300138
  88. Aneuploidy and elevated CEA levels besides increased T category could serve for individual risk assessment to predict metachronous metastases. PMID: 23296402
  89. High CEA expression is associated with lung adenocarcinoma-associated cytologically negative pleural effusions. PMID: 23408492
  90. High serum carcinoembryonic antigen levels are associated with colorectal cancer. PMID: 23765330
  91. CEA improves the accuracy of differential diagnosis in patients with malignant pleural mesothelioma. PMID: 23532816
  92. Further, the data suggest a role for CEACAM1 and CEACAM5 in the phenomenon of increased host susceptibility to bacterial infection upon viral challenge in the human respiratory tract. PMID: 23941132
  93. The colorectal cancer (CRC) neoplastic glands in colon and liver expressed higher amounts of CEA compared with normal colon. CRC neoplastic glands formed in lung expressed low CEA level, but CEA expression was high in areas of tumor necrosis in lung. PMID: 23216017
  94. Results indicate that CEA and CYFRA 21-1 are reliable serum tumor markers for the diagnosis of lung cancer. PMID: 23352032
  95. expression of MVD, LVD, Kiss-1 and CEA mRNA is related to the prognosis of NSCLC PMID: 22681921
  96. Elevated carcinoembryonic antigen was associated with peritoneal carcinomatosis of appendiceal origin. PMID: 22941175
  97. CA19-9 may be a prognostic factor for colorectal cancer patients with normal CEA levels. PMID: 22426691
  98. Patients with normal postoperative CEA levels have a better prognosis. Furthermore, for marginally elevated postoperative CEA level gastric cancer patients, the tumor position and histological grade were two important factors for predicting prognosis. PMID: 23297055
  99. In patients with non-small cell lung cancer, recurrence-free survival differed most significantly at a preoperative CEA level of 2.5 ng/ml. Preoperative CEA >2.5 ng/ml was identified as an independent risk factor for recurrence. PMID: 23192365
  100. High carcinoembryonic antigen expression is associated with metastatic colorectal cancer. PMID: 23449351

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Subcellular Location Cell membrane, Lipid-anchor, GPI-anchor
Protein Families Immunoglobulin superfamily, CEA family
Tissue Specificity Found in adenocarcinomas of endodermally derived digestive system epithelium and fetal colon.
Database Links

HGNC: 1817

OMIM: 114890

KEGG: hsa:1048

STRING: 9606.ENSP00000221992

UniGene: Hs.709196

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