Human dermatopontin,DPT ELISA Kit

Instructions
Code CSB-E11277h
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name dermatopontin
Alternative Names DERM_HUMAN ELISA Kit; Dermatopontin ELISA Kit; Dpt ELISA Kit; TRAMP ELISA Kit; Tyrosine rich acidic matrix protein ELISA Kit; Tyrosine-rich acidic matrix protein ELISA Kit
Abbreviation DPT
Uniprot No. Q07507
Species Homo sapiens (Human)
Sample Types serum, plasma, cell culture supernates, tissue homogenates
Detection Range 0.312 ng/mL-20 ng/mL
Sensitivity 0.078 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human DPT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %92
Range %85-102
1:2Average %91
Range %82-103
1:4Average %95
Range %86-108
1:8Average %94
Range %87-105
Recovery
The recovery of human DPT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9583-104
EDTA plasma (n=4)9587-105
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
202.417 2.493 2.455 2.314
101.763 1.817 1.790 1.649
51.025 0.982 1.004 0.863
2.50.611 0.595 0.603 0.462
1.250.436 0.416 0.426 0.285
0.6250.329 0.307 0.318 0.177
0.3120.238 0.228 0.233 0.092
00.142 0.139 0.141
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Data

Function Seems to mediate adhesion by cell surface integrin binding. May serve as a communication link between the dermal fibroblast cell surface and its extracellular matrix environment. Enhances TGFB1 activity. Inhibits cell proliferation. Accelerates collagen fibril formation, and stabilizes collagen fibrils against low-temperature dissociation (By similarity).
Gene References into Functions
  1. DPT gene silencing could effectively reduce the proliferation of MG-63 cells, slow down the cell cycle progression and promote cell apoptosis. PMID: 29115446
  2. Novel mutation in DPT segregates with the complete phenotype with variable expressivity in two pedigrees with autosomal dominant familial Meniere's disease. PMID: 27876815
  3. This study essentially deals with assessing the expression and integrity of an extracellular matrix protein, Dermatopontin (DPT), in chronic cutaneous wounds. PMID: 25260909
  4. dermatopontin functional peptide produces fibrinogen fibrils and modifies its biological activity PMID: 25082449
  5. These results indicate that DPT can modify certain biological functions of fibrin, and thus a another function of this extracellular matrix protein was revealed. PMID: 23877568
  6. data provided strong evidence that downregulation of DPT is a characteristic event in human oral squamous cell carcinoma and that DPT was correlated with cellular adhesion and invasiveness. PMID: 21796630
  7. DPT has an accelerating role in fibroblast cell adhesion to the provisional matrix in the initial stage of wound healing. PMID: 21398523
  8. suggests that dermatopontin can play various roles in different tissues and might be a molecule related to carcinogenesis and the progression of HCC via possible interaction with TGF-beta1 and other potential mechanisms PMID: 19916908
  9. roles of dermatopontin and histamine receptor H1 genes as downstream targets for the VDR were confirmed by gel electromotility shift and chromatin immunoprecipitation assays that showed the presence of VDR complex binding sequences PMID: 17547532

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Subcellular Location Secreted, extracellular space, extracellular matrix
Protein Families Dermatopontin family
Tissue Specificity Expressed in fibroblasts, heart, skeletal muscle, brain and pancreas. Expressed at an intermediate level in lung and kidney, and at a low level in liver and placenta. Expressed at a lower level in fibroblasts from hypertrophic scar lesional skin and in fi
Database Links

HGNC: 3011

OMIM: 125597

KEGG: hsa:1805

STRING: 9606.ENSP00000356791

UniGene: Hs.80552

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