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Human glucocorticoid receptor-β,GR-β ELISA Kit

Human glucocorticoid receptor-β,GR-β ELISA Kit

Protocol MSDS

Code	CSB-E08889h
Size	96T,5×96T,10×96T
Price	Request a Quote
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Product Details
Customer Reviews and Q&A

Product Details

Target Name

glucocorticoid receptor-β,GR-β

Abbreviation

GR-β

Species

Homo sapiens (Human)

Sample Types

serum, plasma, tissue homogenates, cell lysates

Detection Range

39 pg/mL-2500 pg/mL

Sensitivity

9.75 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Epigenetics and Nuclear Signaling

Assay Principle

quantitative

Measurement

Sandwich

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human GR-β in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	103
	Range %	99-108
1:2	Average %	98
	Range %	94-102
1:4	Average %	91
	Range %	88-95
1:8	Average %	89
	Range %	85-96

Recovery

The recovery of human GR-β spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	90-100
EDTA plasma (n=4)	87	83-95

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
2500	2.441	2.382	2.412	2.301
1250	1.692	1.725	1.709	1.598
625	1.021	1.103	1.062	0.951
312.5	0.563	0.539	0.551	0.440
156	0.327	0.314	0.321	0.210
78	0.245	0.258	0.252	0.141
39	0.185	0.191	0.188	0.077
0	0.108	0.113	0.111

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.

Description

This Human GR-β ELISA Kit was designed for the quantitative measurement of Human GR-β protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 39 pg/mL-2500 pg/mL and the sensitivity is 9.75 pg/mL.

Customer Reviews and Q&A

■ Customer Reviews

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■ Q&A

Please provide the following information for CSB-E08889h
1.What is the standard? (Recombinant full-length protein? Partial protein
(range?)?, peptide (sequence range?), native protein?)
2. If recombinant protein, then what is the host?
3. Is the capture antibody a monoclonal or polyclonal?
4. What is the host of the capture antibody?
5. Is the detection antibody a monoclonal or polyclonal?
6. What is the host of the detection antibody?

Thanks for your inquiry.
CSB-E08889h
Capture antibody is mouse monoclonal antibody.
Detection antibody is goat polyclonal antibody.
Standard is CHO cell-derived recombinant protein.

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