Human hepcidin(Hepc) ELISA Kit

Code CSB-E13062h
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details


The Human hepcidin (Hepc) ELISA Kit is used to quantitatively measure the levels of hepcidin in human serum, plasma, tissue homogenates, or cell lysates. This assay employs the quantitative sandwich enzyme immunoassay technique, in which hepcidin in the samples or standards are sandwiched between pre-coated hepcidin antibody and HRP-conjugated antibody specific for hepcidin. Following a wash to remove any unbound reagent, the TMB substrate solution is added to the wells and color develops in proportion to the amount of hepcidin bound in the initial step. The color development is stopped and the intensity of the color is measured at 450 nm via a microplate reader. This kit displays many advantages, including sensitivity, specificity, precision, linearity, and recovery. The product instructions are access to more information.

Hepcidin, the central regulator of systemic iron homeostasis, tightly influences erythrocyte production. It acts by modulating cellular iron export through ferroportin to plasma and extracellular. Hepcidin binding to ferroportin leads to internalization of the receptor-ligand complex and their eventual degradation. The expression of hepcidin in hepatocytes is regulated by iron-mediated pathways, inflammation signals, and erythropoietic drive. High hepcidin levels block intestinal iron absorption and macrophage iron recycling, causing iron-restricted erythropoiesis and anemia. Low hepcidin levels favor bone marrow iron supply for hemoglobin production and red blood cell synthesis.

Target Name hepcidin antimicrobial peptide
Alternative Names Hamp ELISA Kit; HEPC ELISA Kit; HEPC_HUMAN ELISA Kit; Hepc20 ELISA Kit; Hepc25 ELISA Kit; HEPCIDIN ELISA Kit; Hepcidin 20 ELISA Kit; Hepcidin 25 ELISA Kit; Hepcidin antimicrobial peptide ELISA Kit; Hepcidin-20 ELISA Kit; Hepcidin25 ELISA Kit; HFE2 ELISA Kit; HFE2B ELISA Kit; LEAP 1 ELISA Kit; LEAP-1 ELISA Kit; LEAP1 ELISA Kit; Liver expressed antimicrobial peptide ELISA Kit; Liver-expressed antimicrobial peptide 1 ELISA Kit; PLTR ELISA Kit; Putative liver tumor regressor ELISA Kit
Abbreviation HAMP
Uniprot No. P81172
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range Request Information
Sensitivity Request Information
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cardiovascular
Assay Principle quantitative
Measurement Sandwich
Typical Data   
Materials provided
  • A micro ELISA plate --- The 96-well plate has been pre-coated with anti-human hepcidin antibody.
  • Six vials standard (0.5 ml/bottle)--- Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
  • One vial HRP-conjugated hepcidin antibody (6 ml/bottle) --- Bind to the hepcidin in the samples or standards and react with the substrate to make the solution chromogenic.
  • One vial Wash Buffer (20x concentrate) (15 ml/bottle) --- Wash away unbound or free substances.
  • One vial Substrate A (7ml/bottle) --- Mix with substrate B and then interact with TMB to elicit a chromogenic reaction.
  • One vial Substrate B (7ml/bottle) --- Mix with substrate A and interact with TMB to elicit a chromogenic reaction.
  • One vial Stop Solution (7ml/bottle) --- Stop the color reaction. The solution color immediately turns from blue to yellow.
  • Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
  • An instruction manual
Materials not provided
  • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
  • An incubator can provide stable incubation conditions up to 37°C±5°C.
  • Centrifuge
  • Vortex
  • Squirt bottle, manifold dispenser, or automated microplate washer
  • Absorbent paper for blotting the microtiter plate
  • 50-300ul multi-channel micropipette
  • Pipette tips
  • Single-channel micropipette with different ranges
  • 100ml and 500ml graduated cylinders
  • Deionized or distilled water
  • Timer
  • Test tubes for dilution
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
and FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days


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Target Background

(From Uniprot)
Liver-produced hormone that constitutes the main circulating regulator of iron absorption and distribution across tissues. Acts by promoting endocytosis and degradation of ferroportin/SLC40A1, leading to the retention of iron in iron-exporting cells and decreased flow of iron into plasma. Controls the major flows of iron into plasma: absorption of dietary iron in the intestine, recycling of iron by macrophages, which phagocytose old erythrocytes and other cells, and mobilization of stored iron from hepatocytes.; Has strong antimicrobial activity against E.coli ML35P N.cinerea and weaker against S.epidermidis, S.aureus and group b streptococcus bacteria. Active against the fungus C.albicans. No activity against P.aeruginosa.
Gene References into Functions
  1. Hepcidin is lower in more severely anemic children with sickle cell disease independent of inflammation or markers of erythropoiesis. PMID: 30056060
  2. serum hepcidin level has a role in iron status in children with cystic fibrosis PMID: 30057485
  3. Athree-dimensional (3D) model of hepcidin-25 with bound copper(II) is presented. PMID: 30072660
  4. our data demonstrate that hypoxia strongly potentiates the peroxide-mediated induction of hepcidin via STAT3 signaling pathway. Moreover, oxidases such as NOX4 or artificially overexpressed urate oxidase (UOX) can induce hepcidin PMID: 29459227
  5. High hepcidin maternal serum levels could be an early marker of preeclampsia. PMID: 29523273
  6. Suggest IL-6 can induce hepcidin in systemic lupus erythematosus flares. PMID: 27416847
  7. Serum hepcidin was associated with more severe anemia in advanced CKD patients. PMID: 29227972
  8. Data suggest that magnitude of up-regulation of serum hepcidin levels in response to acute exercise in adults is dependent on pre-exercise iron nutritional status (ferritin levels) and on circulating pro-inflammatory cytokines (prominently interleukin-6). [REVIEW] PMID: 29443922
  9. The present study provides new evidence that ASP decreases hepcidin expression, which can reduce iron burden and inhibit tumor proliferation. These findings might aid ASP developed as a potential candidate for cancer treatment in patients with iron overload. PMID: 29843136
  10. Postexercise supplementation with protein and carbohydrate and vitamins D3 and K2 did not blunt the postexercise hepcidin response in highly trained athletes. PMID: 28605609
  11. A decrease in serum FGF23 and hepcidin levels was observed in chronic hemodialysis patients treated with lanthanum carbonate. PMID: 27928636
  12. These observations suggest correlations between serum hepcidin and progression of chronic HBV infection, and may shed a new light on the development of biomarkers for HBV-related disease surveillance. PMID: 27694815
  13. Increasing adiposity in the pediatric population is associated with increasing IL-6, which stimulates hepcidin synthesis, leading to functional iron deficiency due to inhibition of iron absorption and mobilization from iron stores. PMID: 28604755
  14. Suggest that pentoxifylline may be a clinically and biologically meaningful modulator of hepcidin-25 in dialysis of patients with ESA-hyporesponsive anaemia. PMID: 27171136
  15. Among low birth weight infants, gestational age, IL-6, erythropoietin, and soluble transferrin receptor were associated with Hep25 levels. Therefore, prematurity, inflammation, hypoxia, and erythropoietic activity may be important perinatal factors that affect hepcidin levels. PMID: 28601871
  16. genetic association studies in cohort of infants in Spain: Data suggest that serum hepcidin levels increase in infants during first year of life and are positively associated with iron status only in infants with wild-type HFE gene (not in infants with genetic polymorphisms C282Y, H63D, and S65C). (HFE, homeostatic iron regulator) PMID: 29404719
  17. Data suggest that circulating hepcidin levels (a biomarker for iron-deficiency anemia) may be regulated by dietary factors other than iron; here, one-time high-dose vitamin D3 reduces plasma hepcidin levels in adults one week post-dosing, without changes in plasma pro-inflammatory cytokine or ferritin concentrations. PMID: 27402475
  18. Hepcidin can very well be utilized as a potential prognostic marker to follow patients with breast cancer metastatic to bone. PMID: 28442512
  19. Data suggest that hematologic parameters in children consuming lacto-ovo vegetarian diets are comparable with those of control children, but ferritin levels are lower; inclusion of novel serum biomarkers, soluble transferrin receptor and hepcidin, in nutritional assessment can better detect subclinical iron deficiency in children following vegetarian diet. This study was conducted in Poland with children ages 4.5-9 years. PMID: 28342014
  20. results suggest that lower Hepcidin-25, as well as higher sTfR and sTfR/Hepcidin-25 ratio were significant predictors of favorable hemoglobin response within a month after IV administration of ferric carboxymaltose in patients with CKD PMID: 27282576
  21. unreported iron metabolism-related genes in non-classic hereditary hemochromatosis patients that were predicted to be potentially pathogenic were three novel mutations in TFR2 [two missense (p.Leu750Pro and p.Ala777Val) and one intronic splicing mutation (c.967-1G>C)], one missense mutation in HFE (p.Tyr230Cys), and one mutation in the 5'-UTR of HAMP gene (c.-25G>A) PMID: 27667161
  22. increased intracellular iron content in recombinant-TfR1 HepG2 cells did not increase hepcidin responses compared to wild-type cells, resembling hemochromatosis PMID: 27667164
  23. Review of the role of hepcidin in iron metabolism disorders. PMID: 28073521
  24. The bone morphogenetic protein (BMP) pathway regulates expression of hepcidin through transcriptional activation via BMP-responsive elements (REs) 1 and 2 on the promoter. A search for GC-rich sequences on the hepcidin promoter indicated 13 regions across the distal (A to F), middle (G to I), and proximal (J to M) areas. PMID: 29105755
  25. Of the non-HFE forms of iron overload, TFR2-, HFE2-, and HAMP-related forms are predicted to be rare, with pathogenic allele frequencies in the range of 0.00007 to 0.0005. Significantly, SLC40A1 variants that have been previously associated with autosomal-dominant ferroportin disease were identified in several populations (pathogenic allele frequency 0.0004), being most prevalent among Africans PMID: 26633544
  26. Data (including data from studies using knockout mice) suggest that MT2/TMPRSS6 suppresses hepcidin expression in hepatocytes independently of HJV; MT2/TMPRSS6 cleaves ALK2, ALK3, ACTRIIA, BMPR2, HFE, and, to a lesser extent, HJV and TFR2; thus, MT2/TMPRSS6 suppresses hepcidin expression by cleaving multiple components of the hepcidin induction pathway. (MT2/TMPRSS6 = matriptase-2; HJV = hemojuvelin) PMID: 28924039
  27. two unrelated hepatocellular carcinoma patients bore the HAMP:c.-153C>T mutation at the heterozygous state, which is associated with increased risk of iron overload and severe hemochromatosis PMID: 26474245
  28. Hepcidin-25 released from plaque macrophages and other cell surfaces contributed to the plaque instability by inducing endothelial cell death. PMID: 27855289
  29. Hepcidin plasma levels were increased in patients with early rheumatoid arthritis compared with healthy volunteers. PMID: 28482738
  30. study followed the dynamics of hepcidin-mediated ferroportin internalization; also showed that the novel p.D84E mutation, associated with the classical form of ferroportin disease, is both iron transport defective and hepcidin insensitive PMID: 28681497
  31. During regulation of hepcidin synthesis, multiple promoter elements in the HAMP gene respond to variable signaling pathways corresponding to different extracellular situations. PMID: 28501597
  32. Our findings indicate that the HAMP-P -582A>G polymorphism (rs10421768) is associated with susceptibility to extrapulmonary TB, but not pulmonary TB. CD14+ monocytes from individuals with the rs10421768 GG genotype secreted significantly less hepcidin in response to M. tuberculosis lipoarabinomannan compared with cells from individuals with either the AA or AG genotypes. PMID: 28530443
  33. Expression of Hepcidin and Ferroportin in the Placenta, and Ferritin and Transferrin Receptor 1 Levels in Maternal and Umbilical Cord Blood in Pregnant Women with and without Gestational Diabetes PMID: 27483296
  34. this study shows that hepcidin is involved in the pathogenesis of sepsis-induced acute kidney injury PMID: 27266727
  35. The hepcidin plays a role in the pathogenesis and progression of kidney injury via modulation of iron-mediated oxidant injury. PMID: 28302014
  36. The levels of hepcidin were higher, while those of iron, transferrin, and sTfR were lower in children with cardiometabolic risk factors. PMID: 27139516
  37. Main pathways of systemic and genetic regulation of hepcidin, as well as its influence on the disorders related to iron metabolism. Review. PMID: 28514781
  38. Data suggest hepcidin is the master regulator of systemic iron homeostasis; hepcidin levels are suppressed when erythropoiesis is stimulated; the erythroid-derived hormone erythroferrone appears to be a convincing candidate for link between increased erythropoiesis and hepcidin suppression. [REVIEW] PMID: 27146013
  39. Data suggest that proinflammatory cytokine interleukin-1beta (IL1B) up-regulates hepcidin expression in hepatocytes; inflammation induces IL1B production in Kupffer cells and hepatocytes; IL1B up-regulates CEBPD (CCAAT/enhancer binding protein delta) expression in hepatocytes; up-regulation of CEBPD expression up-regulates hepcidin transcription. PMID: 28438835
  40. modification of HS structure mediated by heparanase overexpression affects hepcidin expression and iron homeostasis PMID: 27711215
  41. Serum hepcidin levels increased in premenopausal women participating in brisk walking exercises. PMID: 28361576
  42. Gene-based meta-analyses revealed 19 genes that showed significant association with hepcidin. Our results suggest the absence of common SNVs and rare exonic SNVs explaining a large proportion of phenotypic variation in serum hepcidin PMID: 27846281
  43. hepcidin induction by endoplasmic reticulum stress involves the central SMAD1/5/8 pathway PMID: 27483343
  44. a selective splicing variant of hepcidin mRNA lacking exon 2 of HAMP gene, producing the transcript that encodes truncated peptide lacking 20 amino acids at the middle of preprohepcidin, was found in hepatocellular carcinoma cell lines PMID: 27264950
  45. Gastric H. pylori infection is a common cause of IDA of unknown origin in adult patients. Our results provide evidence indicating that hepcidin level decreases after successful H. pylori eradication with improvement in IDA. PMID: 26932797
  46. The data of in vitro and in vivo research evidenced on involvement of Hepc in formation of breast cancer cells malignant phenotype and their resistance to doxorubicin. PMID: 27356575
  47. Hepcidin was a more influential determinant of iron stores than blood loss and dietary factors combined, and increased hepcidin diminished the positive association between iron intake and iron stores. PMID: 27598194
  48. These results suggest that the ingestion of a high (compared to low) CHO diet over a seven-day training period is ineffective in attenuating post-exercise IL-6 and hepcidin responses. Such results may be due to the modest training load, the increased protein intake in the low-CHO trial, and a 48 h recovery period prior to sample collection on day 7, allowing a full recovery of muscle glycogen status between exercise sessi PMID: 27379793
  49. The NGAL/hepcidin ratio is more strongly associated with severe of acute kidney injury than the single biomarkers alone PMID: 25479470
  50. serum NGAL and hepcidin levels might be valuable for the evaluation of inflammation in chronic kidney disease, not related through iron metabolism PMID: 26627016

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Involvement in disease Hemochromatosis 2B (HFE2B)
Subcellular Location Secreted.
Protein Families Hepcidin family
Tissue Specificity Highest expression in liver and to a lesser extent in heart and brain. Low levels in lung, tonsils, salivary gland, trachea, prostate gland, adrenal gland and thyroid gland. Secreted into the urine and blood. Expressed by hepatocytes.
Database Links

HGNC: 15598

OMIM: 606464

KEGG: hsa:57817

STRING: 9606.ENSP00000222304

UniGene: Hs.8821

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