Human neutrophil gelatinase-associated lipocalin,NGAL ELISA Kit

Instructions
Code CSB-E09408h
Size 96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name lipocalin 2
Alternative Names 24p3 ELISA Kit; 25 kDa alpha 2 microglobulin related subunit of MMP9 ELISA Kit; 25 kDa alpha-2-microglobulin-related subunit of MMP-9 ELISA Kit; Alpha 2 microglobulin related protein ELISA Kit; HGNC:6526 ELISA Kit; HNL ELISA Kit; Lcn 2 ELISA Kit; Lcn2 ELISA Kit; Lipocalin-2 ELISA Kit; Migration stimulating factor inhibitor ELISA Kit; MSFI ELISA Kit; Neutrophil gelatinase associated lipocalin ELISA Kit; Neutrophil gelatinase associated lipocalin precursor ELISA Kit; Neutrophil gelatinase-associated lipocalin ELISA Kit; NGAL ELISA Kit; NGAL_HUMAN ELISA Kit; Oncogene 24p3 ELISA Kit; Oncogenic lipocalin 24p3 ELISA Kit; p25 ELISA Kit; Siderocalin ELISA Kit; siderocalin LCN2 ELISA Kit; SV40 induced 24P3 protein ELISA Kit; Uterocalin ELISA Kit
Abbreviation NGAL
Uniprot No. P80188
Species Homo sapiens (Human)
Sample Types serum, plasma, urine, tissue homogenates
Detection Range 15.6 pg/mL-1000 pg/mL
Sensitivity 3.9 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Metabolism
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Target Data

Function Iron-trafficking protein involved in multiple processes such as apoptosis, innate immunity and renal development. Binds iron through association with 2,5-dihydroxybenzoic acid (2,5-DHBA), a siderophore that shares structural similarities with bacterial enterobactin, and delivers or removes iron from the cell, depending on the context. Iron-bound form (holo-24p3) is internalized following binding to the SLC22A17 (24p3R) receptor, leading to release of iron and subsequent increase of intracellular iron concentration. In contrast, association of the iron-free form (apo-24p3) with the SLC22A17 (24p3R) receptor is followed by association with an intracellular siderophore, iron chelation and iron transfer to the extracellular medium, thereby reducing intracellular iron concentration. Involved in apoptosis due to interleukin-3 (IL3) deprivation
Gene References into Functions
  1. NGAL is expressed in enteroendocrine cells in small intestine as well as in colon. PMID: 29869714
  2. Urinary NGAL levels were increased in type 2 diabetic patients with early diabetic nephropathy. PMID: 29673928
  3. In the second trimester, high urinary levels indicate tubular injury in gestational diabetes mellitus cases which seems to be a result of hyperglycemia PMID: 28768458
  4. use of bedside NGAL assessment may significantly hasten diagnosis and treatment of CI-AKI PMID: 29791495
  5. neither the inflammatory biomarker calprotectin, nor the tubular biomarkers NGAL and KIM-1, provide robust prognostic information on the loss of renal function in a heterogeneous CKD population. All of them, however, are candidate prognostic biomarkers in primarily inflammatory renal diseases. PMID: 30078006
  6. Plasma NGAL is a valuable biomarker for differentiating between patients with asthma-chronic obstructive pulmonary disorder overlap and asthma patients. PMID: 29580282
  7. There is no significant relationship between breast cancer patients and LCN2 gene polymorphisms (rs11556770). PMID: 29915849
  8. Knockdown of NGAL protein exhibited inhibited proliferation, enhanced apoptosis, decreased expressions of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-kappaB) as well as B-cell lymphoma-2 (Bcl-2) and increased expressions of cysteine-aspartic acid specific protease-9 (caspase-9) and Bcl2-associated X (Bax), as well as repressed tumorigenicity in vivo. PMID: 30308516
  9. Plasma NGAL level measured after return of spontaneous circulation can be an early predictor for the development of acute kidney injury after cardiac arrest. PMID: 29131707
  10. LCN2 might play a role in the cross-talk between bone homeostasis and glucose homeostasis. PMID: 29952418
  11. The serum levels of cystatin C and urine NGAL, urine OPN can be used as a good marker for assessing the renal effect of obesity which can lead end stage renal disease in pediatric population. PMID: 30035656
  12. Measurement of urinary NGAL levels showed an excellent diagnostic performance for discriminating patients with lupus nephritis from systemic lupus erythematosus without nephritis. PMID: 29533691
  13. In ambulatory healthy women, the relationships among serum LCN2 level, bone density, and osteocalcin were confounded by age and serum creatinine. Although LCN2 was positively related with c-terminal telopeptide of type 1 collagen, the correlation was very weak and may not be physiologically relevant. PMID: 29294226
  14. serum NGAL is an excellent marker for the early diagnosis of febrile UTI, with sensitivity and specificity higher than those of urine NGAL. PMID: 28980274
  15. In this study uNGAL failed to predict contrast induced acute kidney injury (CI-AKI)and was an inadequate triage tool to guide an early intervention strategy to prevent CI-AKI PMID: 28128223
  16. Study results suggest that stratum corneum neutrophil gelatinase-associated lipocalin may be useful as an objective biomarker of changes in acne vulgaris symptoms. PMID: 29473202
  17. Increased serum levels of NGAL were associated with loss of graft function in kidney transplant recipients. PMID: 29528011
  18. In Colombian systemic lupus erythematosus patients, urinary NGAL and MCP-1 in addition to anti-C1q antibodies were useful biomarkers for the identification of renal involvement and discrimination of active lupus nephritis. PMID: 29073812
  19. a mutant form (K3Cys) of endogenous lipocalin 2 (LCN2) is filtered by the kidney but can bypass sites of megalin-dependent recapture, resulting in urinary excretion PMID: 27796299
  20. predictive value of plasma neutrophil gelatinase-associated lipocalin (pNGAL) in patients with severe sepsis PMID: 29572184
  21. Results showed the expression levels of NGAL and NGALR significantly downregulated in clear cell renal cell carcinoma samples. PMID: 30138675
  22. The main finding of the present study was that serum NGAL levels paralleled increasing clinical activity of ulcerative colitis evaluated by Mayo score, and it also discriminated different stages of endoscopic severity of this disease. By contrast, only insignificant association of NGAL with endoscopic activity of Crohn's disease was found with no relationships with clinical activity scores. PMID: 29550798
  23. Upon screening of blood mediators in palmoplantar pustular psoriasis patients, LCN2 emerged as being significantly upregulated compared to healthy participants. PMID: 29395573
  24. Neutrophil gelatinase-associated lipocalin concentrations in the urine and serum on the second day of intensive care unit admission could be used to predict the development of acute kidney injury in the following three to seven days in the intensive care unit; however, the cystatin C concentration did not have predictive value PMID: 28142264
  25. In non-chronic kidney disease group, plasma NGAL was not associated with any baseline variables including iron indices PMID: 28389813
  26. We conclude that the underexpression of CRABP1 and the overexpression of LCN2 may be useful diagnostic biomarkers in thyroid tumours with questionable malignity, and the overexpression of LCN2 and C1QL1 may be useful for prognostic purposes. PMID: 29321030
  27. Blood lipocalin-2 is a biomarker for adipose tissue inflammation in HIV patients treated with antiretroviral therapy. PMID: 29304747
  28. This study illustrates the temporal regulation of NGAL in plasma and CSF, which potentially is a useful reference for studies measuring NGAL as biomarker in older individuals. PMID: 29082525
  29. Higher levels of NGAL in the acute kidney injury patients were associated with high-dose regimen of colistin. PMID: 29190605
  30. bile LCN2 level is a potential diagnostic marker for cholangiocarcinoma. PMID: 27782193
  31. Serum lipocalin-2 concentrations and mortality of severe traumatic brain injury PMID: 28943289
  32. Plasma NGAL is a useful marker of interstitial lesions in patients with chronic kidney disease (CKD) and a predictor of further kidney worsening in the early CKD stage. PMID: 28397074
  33. In STEMI patients, plasma post-percutaneous coronary intervention NGAL levels were decreased compared with pre-pPCI NGAL levels, even with the administration of potentially nephrotoxic contrast medium. Post-NGAL levels seemed to be superior to pre-NGAL levels for the prediction of 30-day mortality outcome. PMID: 28737526
  34. We found that MISP, KLF10, KLF15, PPP1R18, and RXRbeta proteins could strongly respond to TPA stimulation and activate LCN2 transcriptional expression. MEK, ERK, JNK, and P38 kinases were involved in the LCN2 transactivation. Furthermore, the MEK-ERK signal pathway plays a major role in this biological process but does not involve PKCalpha signaling. PMID: 29098164
  35. Nonrenal conditions and systemic severity should be considered for improved AKI prediction by NGAL and L-FABP as biomarkers. PMID: 27605390
  36. NGAL may have some value in non-proteinuric patients and increased values have been observed in persons at risk for Mesoamerican nephropathy--{REVIEW} PMID: 27771693
  37. Studied the role of urinary neutrophil gelatinase-associated lipocalin and albuminuria in the diagnosis of early stage diabetic nephropathy. PMID: 28845433
  38. LCN-2 protein overexpression was positively correlated with MMP-9. PMID: 27339395
  39. The measurement of NGAL appears to predict the occurrence of acute kidney injury after off-pump coronary artery bypass grafting surgery PMID: 28701593
  40. NGAL may have a role in early renal dysfunction even in nonalbuminuric T1D patients PMID: 28620620
  41. Elevated cord blood NGAL concentration seems to be a promising, novel marker, even in subclinical forms of acute kidney injury in asphyxiated neonates. PMID: 27590891
  42. Urinary NGAL levels increase soon after shock wave lithotripsy (SWL). Data suggest that NGAL could be used as an early biomarker of tissue injury after SWL. PMID: 27787615
  43. Results suggest that hippocampal LCN2 upregulation might be a potential pathogenic mechanism leading to further disruption of the hippocampus through neurotoxicity and neuroinflammation in vascular dementia (VaD). Astrocytes seem to be the major source of LCN2 in the hippocampus of rodent models and patients of VaD. Control of hippocampal astrocytic LCN2 expression or activity may be important for neuroprotection in VaD. PMID: 28581123
  44. AUC for day one uNGAL was greater for ICU (AUC=0.85) than in-hospital mortality (AUC=0.74). CONCLUSIONS: Day one uNGAL is a highly accurate predictor of ICU, but less so for in-hospital mortality PMID: 28674715
  45. The urine level of NGAL on the first day of amphotericin B administration was more accurate than serum creatinine in predicting acute kidney injury caused by this agent. PMID: 28575880
  46. Serum neutrophil gelatinase-associated lipocalin level was significantly higher among the total patient cohort and those with sepsis than among the controls, also in patients with systemic inflammatory response syndrome without sepsis and patients without systemic inflammatory response syndrome. Plasma level of neutrophil gelatinase-associated lipocalin was significantly elevated in nonsurvivors compared with survivors. PMID: 28445241
  47. On PICU day 1, interleukin-18 predicted acute kidney injury with area under the curve=0.82, but neutrophil gelatinase-associated lipocalin and liver fatty acid binding protein predicted acute kidney injury with area under the curve of less than or equal to 0.69; on PICU day 2, area under the curve was higher. Interleukin-18 and liver fatty acid binding protein on day 1 predicted prolonged acute kidney injury. PMID: 28430754
  48. Resistin and NGAL correlate with expression of endothelial cell adhesion molecules in sepsis. PMID: 28424824
  49. Direct effects of LCN2 on cardiomyocyte size and number and the consistent associations in experimental and human analyses reveal a central role for LCN2 in the ontogeny of cardiac hypertrophy and heart failure PMID: 28615213
  50. Our results suggest that associated serum levels of proMMP-9, NGAL, proMMP-9/NGAL and NE may reflect the state of systemic inflammation in chronic obstructive pulmonary disease related to cigarette smoking. PMID: 28004483
  51. In acute decompensated heart failure patients, an elevated U-NGAL level on the first day of admission was related to the development of clinical acute kidney injury and independently associated with poor prognosis. PMID: 28522674
  52. Urinary NGAL is a good predictor of mortality in established pediatric acute kidney injury (AKI) of heterogeneous etiology and outperforms calprotectin and KIM-1. Urinary calprotectin and KIM-1 predict renal replacement therapy requirement in pediatric AKI. PMID: 28409285
  53. asma NGAL can be more useful than serum procalcitonin, C-reactive protein , and white blood cell levels for identifying acute pyelonephritis in children with febrile urinary tract infections PMID: 27744618
  54. These findings indicate that circulating levels of lipocalin-2 (LCN-2)in patients after acute pancreatitis may play a role in chronic low grade inflammation associated with abdominal adiposity. PMID: 28279688
  55. Neutrophil gelatinase-associated lipocalin may play a dual role during tumorigenetic and developmental processes of endometrial carcinoma. PMID: 27863382
  56. This study shows the find NGAL levels are significantly higher in regions throughout the brain which are commonly affected by AD pathology. PMID: 27716662
  57. Through transcriptome profiling and combined gain- and loss-of-function studies, the authors identified LCN2 as a major downstream effector of TCF7L1 that drives tumor growth. PMID: 28467300
  58. Lipocalin 2 could facilitate proliferation of castration-resistant prostate cancer cells by activating androgen receptor transcriptional activity. PMID: 27602760
  59. Urinary NGAL measured at ICU admission was significantly associated with long-term renal outcomes after hospital discharge in MAKE-free ICU survivors. Urinary NGAL measurements at ICU might be useful to identify a high risk population of kidney disease progression after intensive care. PMID: 26849631
  60. Recent evidence supports the existence of transferrin-independent iron transport mechanisms in the tumor microenvironment, which points to local iron transport proteins such as lipocalin-2 and/or low molecular weight iron-trafficking substances such as siderophores. [review] PMID: 28342790
  61. Urinary NGAL might play role in the pathway of renal tubular damage in patients with autosomal-dominant polycystic kidney disease PMID: 28583565
  62. IL-1beta has a direct effect on NGAL production by tubular epithelial cells. PMID: 27997859
  63. Urinary NGAL is significantly decreased in patients with compared with patients without recurrent urinary tract infections. PMID: 28210838
  64. Serum LCN2 is of any value as steatosis marker in patients with NAFLD. PMID: 27288631
  65. Urinary NGAL was not associated with a patent ductus arteriosus (PDA) day 3 when adjusted for gestational age and gender. A PDA day 3 was not associated with acute kidney injury (AKI) when adjusted for gestational age and gender. Urinary NGAL was not associated with AKI, but was found to be associated with urine albumin and fractional excretion of sodium. PMID: 28068947
  66. Mild-to-moderate kidney dysfunction modifies the relationship between CysC and high-risk coronary lesion, but not NGAL. PMID: 27680774
  67. Serum NGAL might predict the neurological outcomes of out-of-hospital cardiac arrest victims. PMID: 28482803
  68. The results of the present study suggest that even if NGAL values mildly rose after competition, no kidney injury occurred in these highly trained athletes during mountain stages of professional competitions. PMID: 28958144
  69. In children after renal transplantation, urinary NGAL cannot be used as a specific marker for distinguishing acute rejection from other non-rejection causes of AKI. Urinary NGAL was mainly associated with graft function but not with the etiology of AKI. PMID: 28164508
  70. The amniochorion is a source of NGAL and similarly to other inflammatory conditions, this protein may augment the collagenolytic effect of MMP-9 and modulate host-microbe interactions in pregnancies complicated by intra-amniotic infection/inflammation. PMID: 27251223
  71. Our findings collectively support a potential role of LCN2 in cancer cell invasion through the epithelial-to- mesenchymal transition pathway and suggest that LCN2 could be effectively utilized as a lymph node metastasis marker in cervical cancer. PMID: 26840566
  72. We conclude that low BDNF and high LCN2 and NF-L levels are associated with Multiple Sclerosis (MS) pathogenesis, and high IGFBP1level is a biomarker for female MS only, suggesting different MS progression pathways between the sexes. LCN2 is a candidate predictor of response to natalizumab treatment, and NF-L is a candidate predictor of clinically isolated syndrome's (CIS) conversion into MS. PMID: 29108879
  73. Ascites NGAL level may be a biomarker of peritonitis in hospitalized patients with cirrhosis and an independent predictor of short-term in-hospital mortality. PMID: 29098551
  74. Circulating NGAL and MMP-9/NGAL are associated with plaque vulnerability in patients with carotid artery stenosis. Statin treatment could contribute to plaque stabilization by reducing circulating NGAL and MMP-9/NGAL levels. PMID: 28672747
  75. our findings indicated that LCN2 negatively modulated proliferation, EMT and energy metabolism in colorectal cancer (CRC) cells. Accordingly, LCN2 may be a candidate metastasis suppressor and potential therapeutic target in CRC PMID: 28859238
  76. High plasma NGAL correlates with high mortality and myocardial dysfunction in severe sepsis and septic shock. PMID: 27836304
  77. The analysis showed higher urinary NGAL and urinary ATF3 in patients with sepsis-AKI in comparison with patients with sepsis-non-AKI and healthy volunteers. PMID: 28061889
  78. Knockout of Lcn2 attenuated tumor-associated lymphangiogenesis and breast tumor metastasis PMID: 27353364
  79. Lipocalin-2 Promotes Endoplasmic Reticulum Stress and Proliferation by Augmenting Intracellular Iron in Human Pulmonary Arterial Smooth Muscle Cells PMID: 28255266
  80. plasma level not helpful for predicting chronic kidney disease in acute kidney injury patients PMID: 27658322
  81. Acute kidney injury is more common with sepsis than non-infectious systemic inflammatory response syndrome. Plasma NGAL was diagnostic of AKI in both subgroups. PMID: 27062515
  82. This study tested the utility of neutrophil gelatinase-associated lipocalin (NGAL) and cystatin C (CysC) in addition to standard creatinine for early determination of Acute Kidney Injury (AKI) After Heart Operations Using Cardiopulmonary Bypass. The results support the notion that CysC and NGAL should be viewed as comparable for early AKI detection. PMID: 27862029
  83. Data suggest that elevated serum LCN2 level in overweight/obese women may serve as biomarker for risk of developing metabolic syndrome (MetS); serum NGAL levels positively correlated with saturated fatty acid dietary intake; serum NGAL increased proportionally with increase in MetS risk factors. This study was conducted in the Republic of Korea. PMID: 28049882
  84. The role of LCN2 in Iron Homeostasis and Inflammation is reviewed. PMID: 28628361
  85. The results indicate that urine levels of NGAL and retinol binding protein may be independently associated with albuminuria in the diagnosis of type 2 diabetic nephropathy and may serve as novel biomarkers for the identification of type 2 diabetic nephropathy. PMID: 28954270
  86. plasma level predictive of 90-day mortality in decompensated liver cirrhosis PMID: 28545169
  87. clinical significance of serum levels of neutrophil gelatinase-associated lipocalin (sNGAL) as a biomarker for predicting in-hospital outcomes in patients with ST-elevated myocardial infarction (STEMI) PMID: 28742104
  88. Serum and urinary NGAL were higher in the critically ill group that developed acute kidney injury compared to critically ill patients without AKI. PMID: 27288617
  89. LCN-2 expression and serum levels could discriminate IGT from NGT and type 2 diabetes mellitus (T2DM)from IGT obese women and early predicting T2DM among obese women. While, LCN-2 expression level was the independent predictor of IGT in obese women. Combination of both LCN-2 expression and serum levels improved their diagnostic value in early detection of IGT and T2DM among obese women PMID: 28116808
  90. LCN2 modulated the secretion of proinflammatory cytokines in PSC of the PDAC tumor microenvironment, whereas downregulation of LCN2-specific receptor SLC22A17 blocked these effects. Our results reveal how LCN2 acts in the tumor microenvironment links obesity, inflammation, and PDAC development. PMID: 28249896
  91. High NGQAL expression is associated with Cardiovascular disease. PMID: 28207778
  92. Both in our animal model and in human Age-related macular degeneration (AMD), the AKT2-NF-kappaB-LCN-2 signalling axis is involved in activating the inflammatory response. PMID: 28026019
  93. Data suggget that urine neutrophil gelatinase-associated lipocalin (NGAL) may be helpful for detecting inflammation and selecting mesenchymal stem cell (MSC) transplantation timing in ischemia-reperfusion (I/R) kidney injury. PMID: 28338909
  94. effect of lipocalin 2 on thrombotic events in polycythemia vera and essential thrombocythemia patients PMID: 27161323
  95. Results show that LCN2 is highly expressed in colorectal neoplasm;. After knockdown, LCN2 exhibits a strong tumor-suppressive effect by acting as a new upstream inhibitor of the NF-kappaB/snail pathway to control colorectal cancer progression. PMID: 27912767
  96. Lcn2 is involved in the pathogenesis of psoriasis by modulating neutrophil function, and it could serve as a potential target for treating psoriasis PMID: 26979478
  97. NGAL concentrations are elevated in lupus nephritis and reflect the severity of kidney involvement. PMID: 27166308
  98. NGAL can be used to distinguish oliguria due to the functional adaptation from AKIsCr, directing resources to patients more likely to develop AKIsCr PMID: 27505067
  99. The survival function of LCN2 in endometrial carcinoma cells may be exerted through the PI3K pathway and suppression of the p53-p21 pathway. PMID: 27168162
  100. in the current meta-analysis, based on ten prospective studies involving 29366 participants, we evaluated the role of urinary tubular injury markers (NGAL, KIM-1 and NAG) in predicting clinical outcomes including CKD stage 3, end stage renal disease and mortality. PMID: 27907168

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Subcellular Location Secreted
Protein Families Calycin superfamily, Lipocalin family
Tissue Specificity Expressed in bone marrow and in tissues that are prone to exposure to microorganism. High expression is found in bone marrow as well as in uterus, prostate, salivary gland, stomach, appendix, colon, trachea and lung. Not found in the small intestine or pe
Database Links

HGNC: 6526

OMIM: 600181

KEGG: hsa:3934

STRING: 9606.ENSP00000277480

UniGene: Hs.204238

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