Human osteonectin,ON ELISA Kit

This human osteonectin (ON) ELISA kit employs the quantitative sandwich enzyme immunoassay technique to measure the levels of human ON in different samples, including serum, plasma, saliva, or tissue homogenates. The enzyme-substrate chromogenic reaction is also used to amplify the signal and quantify the levels of the analyte through the intensity of the colored product. The color intensity positively correlates with the amount of ON bound in the initial step.

ON, also called SPARC, is encoded by the gene SPOCK2. It is a non-collagenous calcium-binding protein of bone matrix expressed in mineralized tissues including endothelial cells and fibroblasts. ON is involved in the adhesion of osteoblasts and platelets to their extracellular matrix and in early stromal mineralization. ON exerts these effects by binding strongly to type I collagen and hydroxyapatite. It also inhibits cell proliferation through the arrest of cells in the G1 phase of the cell cycle. ON expression is frequently increased in tissue wound repair or fibrotic diseases.