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| Code | CSB-E08760h |
| Size | 96T,5×96T,10×96T |
| Price | Request a Quote or Start an on-line Chat |
| Trial Size |
24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now |
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
| To assess the linearity of the assay, samples were spiked with high concentrations of Human ON in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:20 | Average % | 94 | ||||
| Range % | 87-98 | |||||
| 1:40 | Average % | 96 | ||||
| Range % | 90-102 | |||||
| 1:80 | Average % | 94 | ||||
| Range % | 88-98 | |||||
| 1:160 | Average % | 97 | ||||
| Range % | 92-103 | |||||
| The recovery of Human ON spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 98 | 94-102 | ||||
| EDTA plasma (n=4) | 86 | 80-92 | ||||
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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This human osteonectin (ON) ELISA kit employs the quantitative sandwich enzyme immunoassay technique to measure the levels of human ON in different samples, including serum, plasma, saliva, or tissue homogenates. The enzyme-substrate chromogenic reaction is also used to amplify the signal and quantify the levels of the analyte through the intensity of the colored product. The color intensity positively correlates with the amount of ON bound in the initial step.
ON, also called SPARC, is encoded by the gene SPOCK2. It is a non-collagenous calcium-binding protein of bone matrix expressed in mineralized tissues including endothelial cells and fibroblasts. ON is involved in the adhesion of osteoblasts and platelets to their extracellular matrix and in early stromal mineralization. ON exerts these effects by binding strongly to type I collagen and hydroxyapatite. It also inhibits cell proliferation through the arrest of cells in the G1 phase of the cell cycle. ON expression is frequently increased in tissue wound repair or fibrotic diseases.
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