Human osteonectin,ON ELISA Kit

Code CSB-E08760h
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Description

This human osteonectin (ON) ELISA kit employs the quantitative sandwich enzyme immunoassay technique to measure the levels of human ON in different samples, including serum, plasma, saliva, or tissue homogenates. The enzyme-substrate chromogenic reaction is also used to amplify the signal and quantify the levels of the analyte through the intensity of the colored product. The color intensity positively correlates with the amount of ON bound in the initial step.

ON, also called SPARC, is encoded by the gene SPOCK2. It is a non-collagenous calcium-binding protein of bone matrix expressed in mineralized tissues including endothelial cells and fibroblasts. ON is involved in the adhesion of osteoblasts and platelets to their extracellular matrix and in early stromal mineralization. ON exerts these effects by binding strongly to type I collagen and hydroxyapatite. It also inhibits cell proliferation through the arrest of cells in the G1 phase of the cell cycle. ON expression is frequently increased in tissue wound repair or fibrotic diseases.

Target Name sparc/osteonectin, cwcv and kazal-like domains proteoglycan (testican) 2
Alternative Names and Kazal-like domains proteoglycan 2 ELISA Kit; CWCV ELISA Kit; FLJ97039 ELISA Kit; KIAA0275 ELISA Kit; SPARC/osteonectin ELISA Kit; Sparc/osteonectin cwcv and kazal like domains proteoglycan (testican) 2 ELISA Kit; Sparc/osteonectin cwcv and kazal like domains proteoglycan 2 ELISA Kit; SPOCK 2 ELISA Kit; Spock2 ELISA Kit; Testican 2 ELISA Kit; Testican-2 ELISA Kit; Testican2 ELISA Kit; TICN 2 ELISA Kit; TICN2 ELISA Kit; TICN2_HUMAN ELISA Kit
Abbreviation SPOCK2
Uniprot No. Q92563
Species Homo sapiens (Human)
Sample Types serum, plasma, saliva, tissue homogenates
Detection Range 0.78 ng/mL-50 ng/mL
Sensitivity 0.195 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Neuroscience
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of Human ON in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:20Average %94
Range %87-98
1:40Average %96
Range %90-102
1:80Average %94
Range %88-98
1:160Average %97
Range %92-103
Recovery
The recovery of Human ON spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9894-102
EDTA plasma (n=4)8680-92
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
502.658 2.857 2.758 2.663
252.307 2.208 2.258 2.163
12.51.601 1.543 1.572 1.477
6.250.847 0.867 0.857 0.762
3.120.485 0.474 0.480 0.385
1.560.307 0.319 0.313 0.218
0.780.181 0.188 0.185 0.090
00.094 0.095 0.095
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Background

Function
(From Uniprot)
May participate in diverse steps of neurogenesis. Binds calcium.
Gene References into Functions
  1. candidate susceptibility gene for bronchopulmonary dysplasia; lung expression pattern indicates potential role in alveolarization PMID: 21836138
  2. Neurons are a major source of all of the testican family members in the normal brain. Abundant distribution of testican 2 may contribute to glioma invasion by inactivating other testican family members including N-Tes, which all inhibit MT-MMPs. PMID: 12810672
  3. purification and characterization of a recombinant testican-2 expressed in insect cells; the C1q subcomponent of complement component C1 was identified as a potential interacting partner of testican-2 PMID: 17950619
Subcellular Location Secreted, extracellular space, extracellular matrix.
Tissue Specificity Highly expressed in brain. Also found in lung and testis.
Database Links

HGNC: 13564

OMIM: 607988

KEGG: hsa:9806

STRING: 9606.ENSP00000321108

UniGene: Hs.523009

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