Human thymidylate synthetase,TS ELISA Kit

Code CSB-E10815h
Size 96T,5×96T,10×96T
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Product Details

Target Name
thymidylate synthetase,TS
Alternative Names
d TMP synthase ELISA Kit; EC 2.1.1.45 ELISA Kit; HsT422 ELISA Kit; MGC88736 ELISA Kit; OTTHUMP00000162195 ELISA Kit; Thymidylate synthase ELISA Kit; Thymidylate synthetase ELISA Kit; TMS ELISA Kit; TS ELISA Kit; TSase ELISA Kit; Tyms ELISA Kit; TYMS protein ELISA Kit; Tyms thymidylate synthetase ELISA Kit; TYSY_HUMAN ELISA Kit
Abbreviation
TYMS
Uniprot No.
Species
Homo sapiens (Human)
Sample Types
serum, plasma, cell culture supernates, tissue homogenates
Detection Range
0.312 ng/mL-20 ng/mL
Sensitivity
0.078 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Epigenetics and Nuclear Signaling
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human TS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 87  
Range % 84-91  
1:2 Average % 102  
Range % 99-105  
1:4 Average % 85  
Range % 81-89  
1:8 Average % 94  
Range % 90-98  
Recovery
The recovery of human TS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 102 98-106  
EDTA plasma (n=4) 87 84-90  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected  
20 2.698 2.587 2.643 2.467  
10 2.174 2.198 2.186 2.010  
5 1.595 1.534 1.565 1.389  
2.5 0.866 0.868 0.867 0.691  
1.25 0.551 0.568 0.560 0.384  
0.625 0.365 0.381 0.373 0.197  
0.312 0.281 0.274 0.278 0.102  
0 0.175 0.177 0.176    
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Human TYMS ELISA Kit was designed for the quantitative measurement of Human TYMS protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.312 ng/mL-20 ng/mL and the sensitivity is 0.078 ng/mL.

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Target Background

Function
(From Uniprot)
Contributes to the de novo mitochondrial thymidylate biosynthesis pathway.
Gene References into Functions
  1. The nasopharyngeal carcinoma patients with positive LRP and TS expression may be less sensitive to chemotherapy with DDP + 5-FU. PMID: 15952572
  2. high level of expression associated with poor prognosis in p-stage I adenocarcinoma of the lung PMID: 11804689
  3. Length polymorphism of thymidylate synthase regulatory region in Chinese populations and evolution of the novel alleles PMID: 11989786
  4. Mutants of TS that confer resistance to MTX as well as 5-FU by random as well as site-directed mutagenesis. Review. PMID: 12084458
  5. Thymidylate synthase as a translational regulator of cellular gene expression. Review. PMID: 12084459
  6. rTS gene expression likely plays a role in down-regulating TS through a natural RNA-based antisense mechanism. Review. PMID: 12084460
  7. Induction of thymidylate synthase as a 5-fluorouracil resistance mechanism. Review. PMID: 12084461
  8. Structure-based studies on species-specific inhibition of thymidylate synthase. Review. PMID: 12084462
  9. mutations in TS result in conformational changes that confer 5-fluordeoxyuridine resistance PMID: 12147691
  10. TYMS and MTHFR compete for limiting supplies of folate required for the remethylation of homocysteine. PMID: 12215845
  11. the identified highly conserved protein domains, which occur at the homodimeric interface of thymidylate synthase(TS), represent potential participating sites for binding of TS protein to its mRNA. PMID: 12220503
  12. Enhanced expression mediates resistance of uterine cervical cancer cells to radiation PMID: 12239455
  13. cytomegalovirus infection induces cellular thymidylate synthase gene expression in quiescent human embryonic lung fibroblasts PMID: 12466474
  14. Gene expression is a predictor for the efficacy of fluoropyrimidine-based chemotherapy for metastatic colorectal cancer. PMID: 12576451
  15. Expression predicts the reponse of metastatic colorectal cancer to raltitrexel. PMID: 12576452
  16. Associations between polymorphisms in the thymidylate synthase and serine hydroxymethyltransferase genes and susceptibility to malignant lymphoma. PMID: 12604405
  17. polymorphism and overexpression of Thymidylate synthase mrna is associated with non-small-cell lung cancer PMID: 12640689
  18. Results suggest that the genotyping for thymidylate synthase and methylenetetrahydrofolate reductase polymorphisms may be a useful indicator in determining the appropriate dose of methotrexate in patients with rheumatoid arthritis. PMID: 12684695
  19. Overexpression of thymidylate synthase mediates desensitization for 5-fluorouracil of cervical cancer cells PMID: 12845668
  20. Results describe the kinetic parameters of human recombinant thymidylate synthase (hrTS) with its natural substrate, dUMP, and E-5-(2-bromovinyl)-2(')-deoxyuridine monophosphate (BVdUMP). PMID: 12859954
  21. Findings identify Cys-180 as a critical residue for the in vitro and in vivo translational regulatory effects of human thymidylate synthase. PMID: 12907731
  22. TS and DPD quantitation may be helpful to evaluate prognosis of patients receiving adjuvant 5-FU and that patients with high TS and low DPD may benefit from adjuvant 5-FU chemotherapy in colorectal cancer. PMID: 14519634
  23. High TS expression is a marker of poor prognosis in resected pancreatic cancer. Patients with high intratumoral TS expression benefit from adjuvant therapy. PMID: 14519641
  24. These results indicate that postoperative chemotherapy with 5-FU could be effective for patients with the G-phenotype tumor, since the incidence of intratumoral expression of TS, the target enzyme of 5-FU, is significantly low in G-phenotype tumors PMID: 14576935
  25. There is prognostic significance to TS mRNA expression in breast neoplasms. PMID: 14702180
  26. thymidylate synthase loss of heterozygosity has a role in progression of colorectal cancer PMID: 14760062
  27. Molecular features of thymidylate synthase that control its degradation are examined; the carboxyl-terminal conformational shift is not required for ligand-mediated stabilization; the amino-terminus governs TS stability and its response to ligands. PMID: 14967037
  28. TYMS gene amplification in 23% of 31 5-FU-treated cancers, whereas no amplification was observed in metastases of patients that had not been treated with 5-FU. PMID: 14970324
  29. Over expression of Thymidylate synthase exhibits oncogene-like activity and suggest a link between DNA synthesis and the induction of a neoplastic phenotype PMID: 15093541
  30. 6 bp/1494 polymorphism varies greatly within different ethnic populations and is in linkage disequilibrium with the thymidylate synthase 5' tandem repeat enhancer polymorphism PMID: 15115918
  31. Polymorphism of TYMS partially modifies the risk of esophageal and stomach cancer in patients with smoking habit. PMID: 15244514
  32. Thymidylate synthase inhibition triggers glucose-dependent apoptosis in p53-negative leukemic cells PMID: 15251465
  33. The combination of low expression of TS and induction of p16(INK4a) after chemotherapy can be important indicators of the sensitivity to 5-FU-based chemotherapy in colorectal cancers. PMID: 15260847
  34. Thymidylate synthase polymorphisms are associated with esophageal squamous cell carcinoma and gastric cardiac adenocarcinoma PMID: 15284183
  35. TS in malignant tissue was statistically higher than in normal stromal tissue in both differentiated and undifferentiated types (p < 0.0001). PMID: 15316940
  36. Polymorphisms in the TS gene may contribute to gastric cancer susceptibility. PMID: 15386366
  37. Polymorphisms of reduced folate carrier,aminoimidazole carboxamide ribonucleotide transformylase,and thymidylate synthase genes contribute to the therapeutic response in rheumatoid arthritis patients to methotrexate. PMID: 15457444
  38. The common MTHFR C677T and TS enhancer region polymorphisms were not risk factors for breast cancer in this patient cohort nor were they associated with phenotypic features or with prognosis. PMID: 15510613
  39. Thymidylate synthase polymorphism may have a role in colorectal cancer PMID: 15571262
  40. there is a high level of homology in polymorphic status of thymidylate synthase between normal and malignant colonic tissues PMID: 15571263
  41. thymidylate synthase is inhibited by trifluorothymidine in human tumor cells PMID: 15571283
  42. Thymidylate synthase 5'- and 3'-untranslated region polymorphisms are associated with risk and progression of squamous cell carcinoma of the head and neck PMID: 15585623
  43. Our findings suggest that the TYMS 3'UTR del/del genotype is a significant determinant of elevated RBC folate concentration in a non-smoking population PMID: 15682292
  44. Investigators found a relationship between the TSmRNA expression, evaluated by real-time PCR, and with the TS level determined by immunohistochemical assay in colorectal cancer. PMID: 15736425
  45. High TS1 gene expression is associated with chemotherapy resistance in esophageal cancer PMID: 15788669
  46. Important role for folate deficiency and impaired TS activity in the etiology of esophagel and gastric cancer. PMID: 15817609
  47. Polymorphisms in the 5'- and 3'-untranslated regions are a risk factor for gastric cancer in China. PMID: 15930032
  48. TS polymorphism may be a genetic determinant of plasma homocysteine level in Korean patients with recurrent spontaneous abortion. PMID: 15985285
  49. thymidylate synthase and dihydropyrimidine dehydrogenase are involved in resistance to 5-fluorouracil in human lung cancer cells PMID: 15993511
  50. thymidylate synthase and orotate phosphoribosyl transferase, but not dihydropyrimidine dehydrogenase, are more highly expressed in prostate cancer than in benign prostatic hyperplasia PMID: 15999119

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Subcellular Location
Nucleus. Cytoplasm. Mitochondrion. Mitochondrion matrix. Mitochondrion inner membrane.
Protein Families
Thymidylate synthase family
Database Links

HGNC: 12441

OMIM: 188350

KEGG: hsa:7298

STRING: 9606.ENSP00000315644

UniGene: Hs.369762

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