Mouse Angiopoietin-related protein 6(ANGPTL6) ELISA kit

Code CSB-EL001714MO
Size 96T,5×96T,10×96T How to order?
Trial Size 24T ELISA kits trial application
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Product Details

Description

This Mouse ANGPTL6 ELISA Kit was designed for the quantitative measurement of Mouse ANGPTL6 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 62.5 pg/mL-4000 pg/mL and the sensitivity is 15.6 pg/mL.

Target Name angiopoietin-like 6
Alternative Names Angptl6 ELISA kit; AgfAngiopoietin-related protein 6 ELISA kit; Angiopoietin-like protein 6 ELISA kit; Angiopoietin-related growth factor ELISA kit
Abbreviation ANGPTL6
Uniprot No. Q8R0Z6
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates
Detection Range 62.5 pg/mL-4000 pg/mL
Sensitivity 15.6 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cardiovascular
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse ANGPTL6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:100Average %89
Range %82-96
1:200Average %98
Range %93-102
1:400Average %99
Range %95-107
1:800Average %91
Range %87-95
Recovery
The recovery of mouse ANGPTL6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9184-95
EDTA plasma (n=4)9996-105
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
40002.616 2.793 2.705 2.546
20001.927 2.036 1.982 1.823
10001.287 1.388 1.338 1.179
5000.769 0.729 0.749 0.590
2500.577 0.604 0.591 0.432
1250.394 0.401 0.398 0.239
62.50.247 0.267 0.257 0.098
00.164 0.154 0.159  
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Citations

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Target Background

Function
(From Uniprot)
May play a role in the wound healing process. May promote epidermal proliferation, remodeling and regeneration. May promote the chemotactic activity of endothelial cells and induce neovascularization. May counteract high-fat diet-induced obesity and related insulin resistance through increased energy expenditure.
Gene References into Functions
  1. Since upregulation of ANGPTL6 is induced on metabolic stress, we investigated the hepatic expression of ANGPTL6 by leptin, a representative adipokine of obesity. Mice on a high-fat diet showed increased serum leptin levels and hepatic Angptl6 expression. PMID: 29852166
  2. Upregulation of ANGPTL6 in mouse keratinocytes enhances susceptibility to psoriasis. PMID: 27698489
  3. mitochondrial OXPHOS function governs the expression of ANGPTL6, which is an essential factor for FGF21 production in adipose tissue and cultured adipocytes. PMID: 28184000
  4. In addition to pronounced angiogenesis and epidermal hyperplasia, marked enlargement of dermal lymphatic vessels was observed in UV-B-exposed Angptl6/AGF transgenic mice. PMID: 22132739
  5. we show that (>80%) of the AGF-deficient mice die at about embryonic day 13, while the surviving AGF-deficient mice develop marked obesity, lipid accumulation in skeletal muscle and liver, and insulin resistance accompanied by reduced energy expenditure PMID: 15778720
  6. Our results strongly suggest that AGF is an integrin ligand as well as a mitogenic growth factor and theoretically participates in cutaneous wound healing in a more complex mechanism. PMID: 16806062
  7. Activation of an ERK1/2-eNOS-NO pathway is a crucial signaling mechanism by which angiopoietin-related growth factor increases blood flow through induction of angiogenesis and arteriogenesis. PMID: 18258819

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Subcellular Location Secreted.
Tissue Specificity Highly expressed in the liver, specifically in hepatocytes, and weakly in the heart. Expressed in hematopoietic cells, platelets and mast cells, and detected at wounded skin.
Database Links

KEGG: mmu:70726

STRING: 10090.ENSMUSP00000035784

UniGene: Mm.34019

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