Name: Mouse Epithelial neutrophil activating peptide 78,ENA-78 ELISA Kit
Brand: CUSABIO
SKU: CSB-E08180m

Product Details

Target Name

chemokine (C-X-C motif) ligand 5

Alternative Names

Cxcl5 ELISA Kit; Scyb5C-X-C motif chemokine 5 ELISA Kit; Cytokine LIX ELISA Kit; Small-inducible cytokine B5) [Cleaved into: GCP-2(1-78); GCP-2(9-78)] ELISA Kit

Abbreviation

CXCL5

Uniprot No.

P50228

Species

Mus musculus (Mouse)

Sample Types

serum, plasma, tissue homogenates

Detection Range

62.5 pg/ml - 4000 pg/ml

Sensitivity

15.6 pg/ml

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative

Measurement

Sandwich

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
	Intra-Assay Precision					Inter-Assay Precision
Sample	1		2		3	1		2		3
n	20		20		20	20		20		20
Mean(pg/ml)	516.646		497.903		491.373	484.723		483.883		477.090
SD	0.035		0.033		0.038	0.041		0.041		0.038
CV(%)	7.172		6.286		7.350	8.055		8.039		7.600
TYPICAL DATA These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml		OD1		OD2			Average		Corrected
4000		2.88		2.876			2.878		2.726
2000		2.239		2.235			2.237		2.085
1000		1.263		1.259			1.261		1.109
500		0.646		0.642			0.644		0.492
250		0.342		0.338			0.340		0.188
125		0.249		0.245			0.247		0.095
62.5		0.218		0.214			0.216		0.064		0.064
0		0.154		0.150			0.152

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse ENA-78/CXCL5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

Sample

Serum(n=4)

1:20

Average %

Range %

83-91

1:40

Average %

104

Range %

98-108

1:80

Average %

Range %

87-96

1:160

Average %

Range %

91-99

Recovery

The recovery of mouse ENA-78/CXCL5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range%
Serum (n=5)	90	84-94
EDTA plasma (n=4)	94	87-97

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.

Customer Reviews and Q&A

■ Customer Reviews

Average Rating:

5.0 - 1 reviews

Submit a Review here

Sample type: Cell culture supernatant

Sample species: Mouse

Sample dilution: No dilution

Review: CSB-E08180m was used to detect the supernatant of normal cells and the supernatant of cells treated with il-17a. The OD value of the standard curve was 3.3675-0.064, and the OD value of samples was 0.076-0.183. The detection process was clear and the results were accurate.

By Anonymous

Read more reviews >>

Target Background

Function
(From Uniprot)

May participate in the recruitment of inflammatory cells by injured or infected tissue. GCP-2(1-78) and, more potent, GCP-2(9-78) attract neutrophils and are involved in neutrophil activation.

Gene References into Functions

Identify Cxcl5 as a novel target of AHR-mediated gene expression in primary mouse keratinocytes. PMID: 28973351
IL-17RA regulates CXL-1 and 5 production in the lungs during the adaptive response. PMID: 26871571
STAT3 is required for maximal OSM-induced CXCL5 expression. PMID: 25692402
CXCL5 has a role in neutrophil recruitment in TH17-mediated glomerulonephritis PMID: 24904089
Since adaptive villus growth occurs despite impaired CXCL5 expression and enhanced angiogenesis, this suggests that the growth of new blood vessels is not needed for resection-induced mucosal surface area expansion following massive SBR. PMID: 24888846
CXCL5 regulates pulmonary responses to infection and plays a central role in conferring clock control of inflammation. PMID: 25064128
findings demonstrated that CXCL1 and CXCL5 are increased in circulation with onset of T2D, are produced by islets under stress, and synergistically affect islet function, suggesting that these chemokines participate in pathogenesis of T2D. PMID: 24928936
TLR2-induced epithelial-derived CXCL5 is critical for polymorphonuclear leukocyte-driven destructive inflammation in pulmonary tuberculosis. PMID: 24509076
data suggest that CXCL6 contributes to experimental pulmonary fibrosis, and CXCL6 inhibition might be used to reduce lung toxicity associated with bleomycin treatment. PMID: 23975892
Our data suggest that the differential regulation of the chemokine CXCL5 between osteoblasts and endothelial cells upon FGF2 treatment is involved in Hematopoietic stem cell mobilization from the osteoblast niche or bone marrow to peripheral blood. PMID: 22827607
CXCL5 modulated macrophage activation, increased expression of the cholesterol efflux regulatory protein ABCA1, and enhanced cholesterol efflux activity in macrophages. PMID: 23376791
The omentum is the main site of early neutrophil entry into the peritoneal cavity, where the action of CXCL5 synergizes with matrix metalloproteinases MMP-2 and -9 to promote neutrophil migration during the inflammatory process. PMID: 23225890
CXCL5-dependent signaling cascades are essential for the recruitment of macrophages to the lungs upon subacute second hand smoke exposure. PMID: 22362385
Cxcr2, Cxcl5, and commensal bacteria have critical roles in regulation of the IL-17/G-CSF axis and neutrophil homeostasis at mucosal sites PMID: 22326959
In a mouse model of lung ischemia-induced angiogenesis, LIX predominates in eliciting a pro-angiogenic phenotype among the three ELR+ CXC chemokines (KC, LIX, and MIP-2) studied. PMID: 20144627
regulates chemokine scavenging and pulmonary host defense to bacterial infection PMID: 20643340
The villus growth observed in resection-induced adaptation is associated with increased expression of the chemokine CXCL5 within the lamina propria. PMID: 20471049
LIX is expressed in response to muscle damage in vivo. Its spatial distribution in injured muscle suggests expression by satellite cells. PMID: 12077361
Cloning and genomic localization of Scyb5. PMID: 12439624
The PMN transendothelial migration induced by the activated myocytes is dependent on the production and secretion of the chemokines LIX and KC by the activated myocytes. PMID: 14988231
proliferative effect of LIX is specific towards the primitive hematopoietic cells PMID: 15036238
GCP-2 modulates cellular immunity in part through IL-8Rbeta interactions PMID: 15356099
alveolar epithelial type II (AEII) cells are the primary source of CXCL5 in the rodent lung via TLR4-dependent signaling PMID: 15778492
CXCR1 is a functional receptor for GCP-2/CXCL6 and interleukin-8/CXCL8 in mouse PMID: 17197447
One of the mechanisms whereby endogenous IFN-gamma mitigates the manifestations of CIA consists of inhibiting production of GCP-2, thereby limiting mobilization and infiltration of neutrophils, which are important actors in joint inflammation. PMID: 17200147
unveils a proinflammatory pathway centered on kinin B1 receptor activation of CXCL5 leading to leukocyte trafficking PMID: 17878384
CXCL5, is ectopically expressed in the corneal epithelial cells of Dstn(corn1) mice, and targeting of the receptor for this chemokine inhibits neutrophil recruitment PMID: 18628996
Secretion of CXCL5 by white adipose tissue resident macrophages represents a link between obesity, inflammation, and insulin resistance. PMID: 19356715

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Subcellular Location

Secreted.

Protein Families

Intercrine alpha (chemokine CxC) family

Database Links

KEGG: mmu:20311

STRING: 10090.ENSMUSP00000031318

UniGene: Mm.4660

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Code	CSB-E08180m
Size	96T,5×96T,10×96T
Price	Request a Quote
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Mouse Epithelial neutrophil activating peptide 78,ENA-78 ELISA Kit

Product Details

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Target Background