Product Details

Purity

>97% as determined by SDS-PAGE.

Endotoxin

Less than 1.0 EU/μg as determined by LAL method.

Activity

Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood neutrophils is in a concentration of 10-100 ng/ml.

Target Names

Cxcl5

Uniprot No.

P50228

Research Area

Immunology

Alternative Names

Cxcl5; Scyb5C-X-C motif chemokine 5; Cytokine LIX; Small-inducible cytokine B5) [Cleaved into: GCP-2(1-78); GCP-2(9-78)]

Species

Mus musculus (Mouse)

Source

E.Coli

Expression Region

41-132aa

Complete Sequence

APSSVIAATE LRCVCLTVTP KINPKLIANL EVIPAGPQCP TVEVIAKLKN QKEVCLDPEA PVIKKIIQKI LGSDKKKAKR NALAVERTAS VQ

Mol. Weight

9.8 kDa

Protein Length

Full Length of Mature Protein

Tag Info

Tag-Free

Form

Liquid or Lyophilized powder

Buffer

Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PBS, pH 7.4, 150 mM NaCl.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

5-10 business days

Datasheet & COA

Please contact us to get it.

Description

CXCL5 (LIX) is a potent ELR⁺ CXC chemokine that drives neutrophil recruitment through CXCR2 signaling, making it a critical tool for dissecting innate immune responses in murine models. This tag-free recombinant mouse CXCL5, encompassing the full-length mature sequence (residues 41–132), induces robust chemotaxis of human peripheral blood neutrophils at 10–100 ng/ml, confirming its suitability for neutrophil migration assays, CXCR2-dependent MAPK and NF-κB signaling studies, and in vivo inflammation models. Endotoxin levels below 1.0 EU/μg minimize LPS-driven artifacts that can confound neutrophil activation readouts, while purity exceeding 97% by SDS-PAGE supports use as a reliable reference standard in cytokine detection assays or as a coating antigen for anti-CXCL5 antibody validation by ELISA. Generated in *E. coli*, this protein provides a suitable basis for functional studies where sequence-authentic, contaminant-controlled chemokine is essential.

Target Background

Function

May participate in the recruitment of inflammatory cells by injured or infected tissue. GCP-2(1-78) and, more potent, GCP-2(9-78) attract neutrophils and are involved in neutrophil activation.

Gene References into Functions

Identify Cxcl5 as a novel target of AHR-mediated gene expression in primary mouse keratinocytes. PMID: 28973351
IL-17RA regulates CXL-1 and 5 production in the lungs during the adaptive response. PMID: 26871571
STAT3 is required for maximal OSM-induced CXCL5 expression. PMID: 25692402
CXCL5 has a role in neutrophil recruitment in TH17-mediated glomerulonephritis PMID: 24904089
Since adaptive villus growth occurs despite impaired CXCL5 expression and enhanced angiogenesis, this suggests that the growth of new blood vessels is not needed for resection-induced mucosal surface area expansion following massive SBR. PMID: 24888846
CXCL5 regulates pulmonary responses to infection and plays a central role in conferring clock control of inflammation. PMID: 25064128
findings demonstrated that CXCL1 and CXCL5 are increased in circulation with onset of T2D, are produced by islets under stress, and synergistically affect islet function, suggesting that these chemokines participate in pathogenesis of T2D. PMID: 24928936
TLR2-induced epithelial-derived CXCL5 is critical for polymorphonuclear leukocyte-driven destructive inflammation in pulmonary tuberculosis. PMID: 24509076
data suggest that CXCL6 contributes to experimental pulmonary fibrosis, and CXCL6 inhibition might be used to reduce lung toxicity associated with bleomycin treatment. PMID: 23975892
Our data suggest that the differential regulation of the chemokine CXCL5 between osteoblasts and endothelial cells upon FGF2 treatment is involved in Hematopoietic stem cell mobilization from the osteoblast niche or bone marrow to peripheral blood. PMID: 22827607
CXCL5 modulated macrophage activation, increased expression of the cholesterol efflux regulatory protein ABCA1, and enhanced cholesterol efflux activity in macrophages. PMID: 23376791
The omentum is the main site of early neutrophil entry into the peritoneal cavity, where the action of CXCL5 synergizes with matrix metalloproteinases MMP-2 and -9 to promote neutrophil migration during the inflammatory process. PMID: 23225890
CXCL5-dependent signaling cascades are essential for the recruitment of macrophages to the lungs upon subacute second hand smoke exposure. PMID: 22362385
Cxcr2, Cxcl5, and commensal bacteria have critical roles in regulation of the IL-17/G-CSF axis and neutrophil homeostasis at mucosal sites PMID: 22326959
In a mouse model of lung ischemia-induced angiogenesis, LIX predominates in eliciting a pro-angiogenic phenotype among the three ELR+ CXC chemokines (KC, LIX, and MIP-2) studied. PMID: 20144627
regulates chemokine scavenging and pulmonary host defense to bacterial infection PMID: 20643340
The villus growth observed in resection-induced adaptation is associated with increased expression of the chemokine CXCL5 within the lamina propria. PMID: 20471049
LIX is expressed in response to muscle damage in vivo. Its spatial distribution in injured muscle suggests expression by satellite cells. PMID: 12077361
Cloning and genomic localization of Scyb5. PMID: 12439624
The PMN transendothelial migration induced by the activated myocytes is dependent on the production and secretion of the chemokines LIX and KC by the activated myocytes. PMID: 14988231
proliferative effect of LIX is specific towards the primitive hematopoietic cells PMID: 15036238
GCP-2 modulates cellular immunity in part through IL-8Rbeta interactions PMID: 15356099
alveolar epithelial type II (AEII) cells are the primary source of CXCL5 in the rodent lung via TLR4-dependent signaling PMID: 15778492
CXCR1 is a functional receptor for GCP-2/CXCL6 and interleukin-8/CXCL8 in mouse PMID: 17197447
One of the mechanisms whereby endogenous IFN-gamma mitigates the manifestations of CIA consists of inhibiting production of GCP-2, thereby limiting mobilization and infiltration of neutrophils, which are important actors in joint inflammation. PMID: 17200147
unveils a proinflammatory pathway centered on kinin B1 receptor activation of CXCL5 leading to leukocyte trafficking PMID: 17878384
CXCL5, is ectopically expressed in the corneal epithelial cells of Dstn(corn1) mice, and targeting of the receptor for this chemokine inhibits neutrophil recruitment PMID: 18628996
Secretion of CXCL5 by white adipose tissue resident macrophages represents a link between obesity, inflammation, and insulin resistance. PMID: 19356715

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Subcellular Location

Secreted.

Protein Families

Intercrine alpha (chemokine CxC) family

Database Links

KEGG: mmu:20311

STRING: 10090.ENSMUSP00000031318

UniGene: Mm.4660

Code	CSB-AP001121MO
Abbreviation	Recombinant Mouse Cxcl5 protein (Active)
MSDS	MSDS Datasheet
Size	$142
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Recombinant Mouse C-X-C motif chemokine 5 protein (Cxcl5) (Active)

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