Product Details

Target Name

alpha-2-HS-glycoprotein

Alternative Names

Ahsg ELISA kit; FetuaAlpha-2-HS-glycoprotein ELISA kit; Countertrypin ELISA kit; Fetuin-A ELISA kit

Abbreviation

AHSG

Uniprot No.

P29699

Species

Mus musculus (Mouse)

Sample Types

serum, plasma, tissue homogenates

Detection Range

6.25 ng/mL-400 ng/mL

Sensitivity

1.56 ng/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Metabolism

Assay Principle

quantitative

Measurement

Sandwich

Precision

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean(ng/ml)	52.627	50.900	51.353	52.901	49.725	50.990
SD	0.041	0.035	0.037	0.054	0.049	0.047
CV(%)	5.216	4.563	4.793	6.844	6.499	6.120

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse fetuin A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

Sample

Serum(n=4)

1:10

Average %

Range %

87-103

1:20

Average %

Range %

84-105

1:40

Average %

Range %

83-103

1:80

Average %

Range %

82-107

Recovery

The recovery of mouse fetuin A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	90-98
Heparin plasma (n=4)	91	85-96

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.



ng/ml	OD1	OD2	Average	Corrected
400	2.657	2.704	2.681	2.547
200	1.905	2.002	1.954	1.820
100	1.259	1.279	1.269	1.135
50	0.714	0.758	0.736	0.602
25	0.426	0.442	0.434	0.300
12.5	0.361	0.348	0.355	0.221
6.25	0.257	0.244	0.251	0.117
0	0.134	0.133	0.134

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.

Target Background

Function
(From Uniprot)

Probably involved in differentiation.

Gene References into Functions

Downregulation of adiponectin in inflamed adipocyte by fetuin-A through the mediation of Wnt3a and PPARgamma is a new report. PMID: 27720679
FETUA expression and ER stress markers in the liver and serum of HFD mice were decreased by melatonin treatment. In conclusion, melatonin can improve hepatic insulin resistance and hepatic steatosis through reduction in ER stress and the resultant AHSG expression. PMID: 29607540
Impairment of energy sensors, SIRT1 and AMPK, in lipid induced inflamed adipocyte is regulated by Fetuin A. PMID: 29030114
The growth plate slippage occurred in 70% of Ahsg-/- mice of both sexes around three weeks postnatal. PMID: 29088242
AHSG is produced in bone, mainly in osteocytes and also show for the first time that its production is modulated by FGF23. PMID: 26476373
These findings establish urinary Fetuin-A as a sensitive biomarker of the progression of autosomal dominant polycystic kidney disease. PMID: 25888842
Plasma levels of the calcification inhibitor fetuin-A were significantly decreased in CKD animals compared to controls. PMID: 24994603
fetuin-A regulates endochondral ossification through mineralization inhibition and lipid (hormone) binding [review] PMID: 23277412
Our results show that CSF fetuin-A is a biomarker of disease activity and natalizumab response in multiple sclerosis PMID: 23439582
lipid-induced FetA from adipocytes is an efficient chemokine for macrophage migration and polarization PMID: 23943623
FetA, via its terminal galactoside moiety, directly binds Leu100-Gly123 & Thr493-Thr516 in Tlr4, fulfilling the requirement of an endogenous ligand for TLR4 through which lipids induce insulin resistance. PMID: 22842477
Our results show that fetuin-A has a developmental rather than a structural role in murine bone. PMID: 23091616
Fetuin-A-containing calciprotein particles facilitate the clearance of mineral debris by macrophages via SR-A. PMID: 22753077
ER stress induced by high glucose and palmitate increases the expression of fetuin-A and further contributes to the development of insulin resistance PMID: 22619360
fetuin-A is protective against lethal systemic inflammation partly by inhibiting active HMGB1 release PMID: 21347455
Fetuin-A deficiency is associated with soft tissue calcification in mice and humans. PMID: 21659653
breast cancer but also of other tumors whose initiation and promotion are under the tight control of TGF-beta. PMID: 20847285
Fetuin-A expressions were negatively correlated with the liver pathological scores and TNFa levels in fulminant hepatic failure. PMID: 18578995
alpha 2-HS glycoprotein/fetuin, a transforming growth factor-beta/bone morphogenetic protein antagonist, regulates postnatal bone growth and remodeling PMID: 11901155
Improved insulin sensitivity and resistance to weight gain in knockout mice PMID: 12145157
role as a systemically acting inhibitor of ectopic calcification PMID: 12897203
Regulation of BMP-induced ectopic bone formation by Ahsg. PMID: 15885488
Onset of malaria infection is delayed in fetuin-A-deficient mice compared to that in wild-type C57BL/6 mice challenged with Plasmodium berghei sporozoites PMID: 16113307
Fetuin-A knockout (fetuin-KO) mice spontaneously develop widespread soft tissue calcification, including significant myocardial calcification, whereas larger arteries are spared. PMID: 16177000
The absence of fetuin may contribute to the improvement of insulin sensitivity associated with aging. PMID: 17011519
Fetuin-A deficiency, chronic kidney disease, and high phosphate diet act synergistically in the pathogenesis of extraosseous calcification. PMID: 17389622
fetuin recognized by anti-LY-R antibodies is an immunogenic form associated only with the tumorigenic LY-R cells and might be involved in tumor progression in this lymphoma. PMID: 19194829
inhibits pathologic calcification in both the soft tissue and vasculature, even in the setting of atherosclerosis PMID: 19389852
inhibition of ectopic calcification PMID: 12556469
inhibition of ectopic calcification PMID: 9395485

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Subcellular Location

Secreted.

Protein Families

Fetuin family

Tissue Specificity

Liver is the major site of synthesis, but fetuin is also expressed in limb buds and other extrahepatic tissues during development.

Database Links

KEGG: mmu:11625

STRING: 10090.ENSMUSP00000023583

UniGene: Mm.197554

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Code	CSB-E14042m
Size	96T,5×96T,10×96T
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Mouse Fetuin A ELISA Kit

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Target Background