Mouse Glucagon (GC) ELISA Kit

Code CSB-E15775m
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Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P55095
Lead Time 3-5 working days
Abbreviation GCG
Target Name glucagon
Alias GLP1, GLP2, GRPP, glicentin-related polypeptide|glucagon-like peptide 1|glucagon-like peptide 2
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates
Detection Range 1.56 pg/mL-100 pg/mL
Sensitivity 0.39 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Metabolism
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details This protein is actually a preproprotein that is cleaved into four distinct mature peptides. One of these, glucagon, is a pancreatic hormone that counteracts the glucose-lowering action of insulin by stimulating glycogenolysis and gluconeogenesis. Glucagon is a ligand for a specific G-protein linked receptor whose signalling pathway controls cell proliferation. Two of the other peptides are secreted from gut endocrine cells and promote nutrient absorption through distinct mechanisms. Finally, the fourth peptide is similar to glicentin, an active enteroglucagon.
HGNC 4191
RGD 2668
MGI 95674
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse GC in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:5Average %92
Range %88-98
1:10Average %97
Range %93-110
1:20Average %95
Range %88-100
1:40Average %95
Range %82-104
Recovery
The recovery of mouse GC spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9485-99
EDTA plasma (n=4)9586-102
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
1002.564 2.592 2.578 2.489
501.439 1.488 1.464 1.375
250.805 0.792 0.799 0.710
12.50.421 0.432 0.427 0.338
6.250.214 0.223 0.219 0.130
3.120.146 0.150 0.148 0.059
1.560.102 0.105 0.104 0.015
00.088 0.090 0.089
References
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Function Glucagon plays a key role in glucose metabolism and homeostasis. Regulates blood glucose by increasing gluconeogenesis and decreasing glycolysis. A counterregulatory hormone of insulin, raises plasma glucose levels in response to insulin-induced hypoglycemia (By similarity).
Subcellular Location Secreted
Protein Families Glucagon family
Tissue Specificity Glucagon is secreted in the A cells of the islets of Langerhans. GLP-1, GLP-2, oxyntomodulin and glicentin are secreted from enteroendocrine cells throughout the gastrointestinal tract. GLP-1 and GLP-2 are also secreted in selected neurons in the brain.
Database Links

KEGG: mmu:14526

STRING: 10090.ENSMUSP00000099794

UniGene: Mm.45494

Pathway Glucagon signaling pathway

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