Mouse Growth/differentiation factor 15(GDF15) ELISA kit

Instructions
Code CSB-EL009345MO
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name growth differentiation factor 15
Alternative Names Gdf15 ELISA Kit; Mic1 ELISA Kit; SbfGrowth/differentiation factor 15 ELISA Kit; GDF-15 ELISA Kit; Macrophage inhibitory cytokine 1 ELISA Kit; MIC-1 ELISA Kit
Abbreviation GDF15
Uniprot No. Q9Z0J7
Species Mus musculus (Mouse)
Sample Types serum, plasma, cell culture supernates, tissue homogenates
Detection Range 7.8 pg/mL-500 pg/mL
Sensitivity 1.95 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse GDF15 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %92
Range %88-96
1:2Average %95
Range %91-99
1:4Average %92
Range %87-98
1:8Average %93
Range %88-96
Recovery
The recovery of mouse GDF15 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9490-98
EDTA plasma (n=4)9186-98
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
5002.254 2.111 2.183 2.020
2501.636 1.694 1.665 1.502
1251.022 1.112 1.067 0.904
62.50.575 0.603 0.589 0.426
31.20.334 0.337 0.336 0.173
15.60.228 0.237 0.233 0.070
7.80.201 0.215 0.208 0.045
00.160 0.166 0.163  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days

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Target Background

Gene References into Functions
  1. GDF15 is a myokine secreted by aging-related stress and may control aging phenotype PMID: 29491220
  2. Membrane-protein extraction and use of an intracellular protein-transport inhibitor showed that GDF-15 promoted CaV3.1 and CaV3.3 alpha-subunit expression by trafficking to the membrane. PMID: 27353765
  3. CHOP activates Gdf15 transcription via binding to its promoter upon endoplasmic reticulum stress. PMID: 28847729
  4. The ANT1-deficient muscle mitochondria produce excess reactive oxygen species (ROS) and are partially uncoupled. Hence, the muscle respiration under nonphosphorylating conditions is increased. Muscle transcriptome analysis revealed the induction of mitochondrial biogenesis, down-regulation of diabetes-related genes, and increased expression of the genes encoding the myokines FGF21 and GDF15. PMID: 28223503
  5. isolating GFRAL as the receptor for GDF15-induced anorexia and weight loss, the identification of a mechanistic basis for the non-homeostatic regulation of neural circuitry by a peripheral signal associated with tissue damage and stress; findings provide opportunities to develop therapeutic agents for the treatment of disorders with altered energy demand PMID: 28953886
  6. These data suggest that GDF15 is required for IL-13-induced improvement of glucose intolerance in mice fed an HFD. PMID: 28874416
  7. HIF-1alpha and GDF15 expression are inversely related under normoxia and hyperoxia. PMID: 28734801
  8. This is the first study defining the role of Mic-1/Gdf15 in a number of behavioural domains. PMID: 28081177
  9. epithelial restitution was promoted by enhanced NAG-1 expression and subsequent enterocyte locomotion during the early wound-healing process PMID: 27421482
  10. GDF15 is a potent mitohormetic signal that safeguards against the onset of obesity and insulin resistance. PMID: 27986797
  11. The aim of this study was to identify the role of endogenous Gdf-15 in the MPTP (1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine) mouse model of Parkinson's disease (PD) by comparing Gdf-15 (+/+) and Gdf-15 (-/-) mice. PMID: 27115420
  12. The results of this study indicated the importance of Gdf-15 in promoting survival of DAergic neurons and regulating the inflammatory response post 6-OHDA lesion. PMID: 26733415
  13. endogenous Growth/differentiation factor-15 is beneficial for axon regeneration following sciatic nerve crush PMID: 26077927
  14. repeated cisterna magna administration of human umbilical cord blood-derived mesenchymal stem cells enhanced endogenous adult hippocampal neurogenesis and synaptic activity through a paracrine factor of GDF-15 PMID: 26154268
  15. Levels of GDF-15 mRNA increased 2 h after induction of ventilator-induced lung injury in WT lungs. Non-hematopoietic cells are the main source of GDF-15. GDF-15 is required for platelet-leukocyte interactions during ventilator-induced lung injury. PMID: 25948320
  16. hNAG-1 appears to be a novel therapeutic target in preventing and treating obesity and insulin resistance. PMID: 24531647
  17. Collectively, hNAG-1 is an important regulator of mammalian longevity and may act as a survival factor. PMID: 25239873
  18. Counterbalance between MIC-1 and ATF3 is critical for deciding the fate of enterocytes under the food chemical stress. PMID: 25180886
  19. Growth differentiation factor 15, a marker of lung involvement in systemic sclerosis, is involved in fibrosis development but is not indispensable for fibrosis development. PMID: 24504814
  20. Growth/differentiation factor 15 promotes EGFR signalling, and regulates proliferation and migration in the hippocampus of neonatal and young adult mice. PMID: 24496615
  21. GDF-15 is therefore prominently upregulated in the retina after optic nerve crush but does not directly interfere with the magnitude and temporal progression of retinal ganglion cell death. PMID: 23640134
  22. GDF15 protects the renal interstitium and tubular compartment in experimental type 1 and 2 diabetes without affecting glomerular damage PMID: 23986522
  23. Enteropathogenic Escherichia coli enhances MIC-1 gene expression in the human intestinal cancer cells PMID: 23503457
  24. knockdown of MIC-1 can decrease RNPC1-induced cell growth suppression. PMID: 23836903
  25. This is the first study that demonstrates a protective role of GDF-15 in advanced atherosclerosis and macrophage accumulation, possibly due to the reduced expression of adhesion molecules. PMID: 23800095
  26. MIC-1/GDF15 is involved in the physiological regulation of appetite and energy storage. PMID: 23468844
  27. GDF-15 specifically inhibits platelet integrin activation, preventing thrombus formation. PMID: 23231375
  28. GDF-15 is involved in orchestrating atherosclerotic lesion progression by regulating apoptotic cell death and IL-6-dependent inflammatory responses to vascular injury. PMID: 23316317
  29. Increased expression of the TGF-b superfamily cytokine MIC-1/GDF15 protects ApoE(-/-) mice from the development of atherosclerosis. PMID: 22386250
  30. GDF15 impairs aortic contractile and relaxing function through an endothelium-dependent mechanism involving altered caveolar endothelial NO synthase signaling. PMID: 23262134
  31. MIC-1/GDF15 has complex actions on tumor behavior PMID: 22952779
  32. identified the secreted protein follistatin-like 1 (FSTL1) as an inducer of GDF15 production. PMID: 22675198
  33. The work shows that the molecule MIC-1/GDF15 might be beneficial for the treatment of obesity as well as perturbations in glucose homeostasis. PMID: 22514681
  34. These findings suggest that GDF15 could play a pivotal role in the pathogenesis of bone loss relevant to hypoxia. PMID: 22190281
  35. coronary artery ligation in Gdf15-deficient mice led to enhanced recruitment of polymorphonuclear leukocytes into the infarcted myocardium and an increased incidence of cardiac rupture PMID: 21516086
  36. Regulation of GDF-15, a distant TGF-beta superfamily member, in a mouse model of cerebral ischemia. PMID: 21128084
  37. GDF-15 deletion has a beneficial effect both in early and later atherosclerosis by inhibition of CCR2-mediated chemotaxis and by modulating cell death. PMID: 21242297
  38. GDF-15 at endogenous levels contributes to proliferation and immune escape of malignant gliomas in an immunocompetent host. PMID: 20534737
  39. Data show that MIC-1 is secreted from melanoma cells together with VEGF to promote vascular development mediated by (V600E)B-Raf signaling. PMID: 20431030
  40. GDF-15 prevents apoptosis in cerebellar granule neurons by activating Akt and inhibiting endogenously active ERK PMID: 12514175
  41. GDF-15/MIC-1 is an early mediator of the injury response in kidney and lung PMID: 15897808
  42. GDF-15 induction is an immediate early response to liver injury that can occur through TNF and p53 independent pathways PMID: 16154591
  43. GDF15 is a novel autocrine/endocrine factor that antagonizes the hypertrophic response and loss of ventricular performance. PMID: 16397142
  44. gdf15 is a growth factor with dual function either promoting proliferation or growth arrest and differentiation due most likely to differences in cellular differentiation PMID: 17286605
  45. GDF-15 has no apparent effect on hepatocellular carcinoma (HCC) tumor formation rate, growth rate or invasiveness in diethylnitrosamine-induced HCC in vivo. PMID: 18210153
  46. Data conclude that MIC-1 is expressed in adipose tissue and secreted from adipocytes and is therefore a new adipokine. MIC-1 may have a paracrine role in the modulation of adipose tissue function and body fat mass. PMID: 19074584
  47. a possible inverse relationship exists between the expression of NAG-1 and COX-2 in tumor formation of colon tissue. PMID: 19375854
  48. the NAG-1 protein inhibits urethane-induced tumor formation PMID: 19401523
  49. Despite striking similarities in the GDF-15 and CNTF knock-out phenotypes, expression levels of CNTF and other neurotrophic factors in the sciatic nerve were unaltered suggesting that GDF-15 is a genuine novel trophic factor for motor and sensory neurons. PMID: 19864576

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Subcellular Location Secreted
Protein Families TGF-beta family
Tissue Specificity Highly expressed in liver (PubMed:10779363, PubMed:28572090, PubMed:29046435). Detected in plasma (at protein level) (PubMed:28572090, PubMed:29046435). Expressed by cardiomyocytes, expression is highly increased in heart diseases (PubMed:28572090). Also
Database Links

KEGG: mmu:23886

STRING: 10090.ENSMUSP00000003808

UniGene: Mm.31325

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