Mouse Interleukin 15,IL-15 ELISA Kit

Instructions
Code CSB-E04604m
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name interleukin 15
Alternative Names Il15 ELISA Kit; Interleukin-15 ELISA Kit; IL-15 ELISA Kit
Abbreviation IL15
Uniprot No. P48346
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates
Detection Range 6.25 pg/mL-400 pg/mL
Sensitivity 1.56 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse IL-15 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 88  
Range % 80-100  
1:2 Average % 98  
Range % 85-105  
1:4 Average % 96  
Range % 92-105  
1:8 Average % 89  
Range % 86-98  
Recovery
The recovery of mouse IL-15 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 92 89-100  
EDTA plasma (n=4) 93 90-99  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
400 2.800 2.805 2.803 2.652  
200 2.148 2.158 2.153 2.002  
100 1.358 1.330 1.344 1.193  
50 0.843 0.788 0.816 0.665  
25 0.458 0.468 0.463 0.312  
12.5 0.302 0.322 0.312 0.161  
6.25 0.212 0.224 0.218 0.067  
0 0.146 0.156 0.151    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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 Q&A
Q:

I am having a question concerning your murine IL-15 ELISA kit. I would like
to know, if this kit detects both single-peptide IL-15 and IL-15/Ra complex, or only
either of the forms? I would highly appreciate if you could clear this question for me.
since I would need a kit that detects the both forms.

A:
Thanks for your inquiry.
Our kit CSB-E04604m detects total mouse IL-15, including another form or the receptor binding IL-15 form.

Target Data

Function Cytokine that stimulates the proliferation of T-lymphocytes. Stimulation by IL-15 requires interaction of IL-15 with components of IL-2R, including IL-2R beta and probably IL-2R gamma but not IL-2R alpha.
Gene References into Functions
  1. SINTBAD regulates IL-15-induced autophagy and NKT cell survival. PMID: 30022064
  2. this study shows that IL-15 supports the generation of protective lung-resident memory CD4 T cells PMID: 29186108
  3. The small intestinal lamina propria innate lymphoid cells subset are not completely IL-7R dependent, but can persist partially through IL-15 signalling. PMID: 28361874
  4. IL-15, mainly produced by non-hematopoietic cells in lung tissue and being trans-presented, promoted inflationary T cell survival by increasing expression of Bcl-2 PMID: 29652930
  5. This review provides significant characterization of specific systemic, cellular and molecular alterations caused by IL-15 superagonist treatment as compared to native IL-15 and compares their anti-tumor effectiveness in multiple preclinical studies [Review] PMID: 28888485
  6. IL-15 trans-presentation by non-CD4 T cells is the primary mechanism via which IL-15 controls CD4 effector T cell differentiation. PMID: 28807496
  7. the protective effect on metastasis was lost upon patrolling monocyte or NK cell depletion, IL15 neutralization, or IFNgamma ablation. The combined analysis of these approaches allowed us to establish a hierarchy in which patrolling monocytes, making IL15 in response to primary tumors, activate NK cells and IFNg production that then inhibit lung metastasis formation. PMID: 28811289
  8. an IL-15 isoform lacking exon-6, IL-15DeltaE6, generated by alternative splicing events of activated immune cells, including macrophages and B cells, is reported. PMID: 27166125
  9. IL-15, but not IL-15Ralpha, is required for the development of spontaneous and virus-induced Type 1 diabetes. PMID: 26853723
  10. hetIL15 administration improves the outcome of Adoptive cell transfer in lymphoreplete hosts, a finding with significant implications for improving cell-based cancer immunotherapy strategies. PMID: 27986749
  11. These data demonstrate that mice with an endogenous IL-15 deficiency are susceptible to the development of severe, enhanced Th2-mediated allergic airway disease, which can be regulated by CD8(+) T cells. PMID: 28093832
  12. IL-15 induces the activation and survival of effector immune cells that are necessary for its antitumoral activity; but, long-term exposure to IL-15 is associated with the development of important side effects mainly mediated by IFN-gamma-producing T-cells PMID: 27356750
  13. findings indicated that IL-15 plays an important role in preventing leukemia development. PMID: 26876593
  14. NK cells activated by IL-4 in cooperation with IL-15 exhibit distinctive characteristics with enhanced immunologic cytotoxicity. PMID: 27551096
  15. Genetic ablation of the IL2Rbeta chain on CD8(+) T cells restrains inhibitory receptor induction, in particular 2B4 and Tim-3; precludes terminal differentiation of highly defective PD-1(hi) effectors; and rescues memory T-cell development and responsiveness to IL-7-dependent signals. Together, we ascribe a previously unexpected role to IL-2 and IL-15 as instigators of CD8(+) T-cell exhaustion during chronic viral infe... PMID: 27573835
  16. this study reveals the function of IL-15 in astrocyte survival via Akt phosphorylation in response to OGD-induced damage. PMID: 28110119
  17. Our new compelling findings have established the critical roles of both IL-7 and IL-15 in the maintenance of memory Th17 cells, and the previously undescribed therapeutic advantages of targeting IL-7 and IL-15 further support a promising clinical translation in Th17 cell-mediated autoimmune and inflammatory disorders. PMID: 27899224
  18. results show that IL-15 is implicated in the high fat diet-induced lipid accumulation and inflammation in the liver, leading to fatty liver disease PMID: 26868085
  19. homeostasis of IL-15-dependent lymphocyte subsets is also regulated by trans-presentation of IL-15 by non-hematopoietic cells in the tissue environment. PMID: 26778709
  20. Recombinant IL-15 is a promising agent for cancer immunotherapy both in humans and animals. PMID: 28286036
  21. Taken together, these data suggest that CCR7 biases memory CD8 T cells toward IL-7-dependent niches over IL-15-dependent niches, which provides insight into the homeostatic regulation of different memory T-cell subsets. PMID: 27385825
  22. results demonstrate that IL-15 and RANKL induce osteoclastogenesis synergistically, and IL-15 might play a novel and major role in destructive inflammatory bone diseases PMID: 27608420
  23. This study provides mechanistic insight into how IL-15 controls the generation of memory CD8 T cells and influences their trafficking and ability to take up residence within peripheral tissues. PMID: 28814601
  24. findings extend understanding of the differential modes of action between IL-2 and IL-15, and highlight how, although sharing the CD122/CD132 receptor, they elicit such different immune actions PMID: 28507024
  25. IL-15 induced by inflammatory signals in response to lymphodepletion drives lymphocyte responses, as memory CD8 T cells proliferated in an IL-15-dependent manner. PMID: 27183627
  26. these findings unveil a role for IL-15 as a critical mediator in defense against systemic candidiasis and hold promise for the design of IL-15-based antifungal immunotherapies. PMID: 28636955
  27. developmental arrest of Natural killer cells results from a lack of IL-15 availability in the microenvironment. PMID: 27837016
  28. IL-15 impacts butyrate-producing bacteria and lowers butyrate levels in the absence of overt pathology, which represent events that precede and promote intestinal inflammatory diseases. PMID: 27648810
  29. endogenous IL-15 does not directly augment the pathogenesis of sepsis but enables the development of septic shock by maintaining NK cell numbers and integrity. PMID: 28031340
  30. this study shows that IL-15-mediated control of the precursor population affects the development of virus-specific CD8+ T-cell memory PMID: 27457412
  31. this study shows that unleashed trans-presentation of IL-15 on MyD88-deficient fibroblastic reticular cells during viral infection fosters the activation of cytotoxic and non-cytotoxic group 1 innate lymphoid cells and accelerates clearance of the virus PMID: 27798617
  32. this study shows that synergistic enhancement of adaptive immunity by the combination of epigallo catechin gallate and vitamin A or E is dependent on both IL-15 and its receptor PMID: 27135790
  33. Under steady-state conditions, KLF2-deficient NK cells alter their expression of homeostatic homing receptors and subsequently undergo apoptosis due to IL-15 starvation. PMID: 27114551
  34. The induction of IgM and IgA, which can play pivotal roles in mucosal immunity, was promoted in the presence of IL-15. Collectively, the data implicate IL-15 as the master cytokine that induces B-1a cells to mount a mucosal immune response. PMID: 25748019
  35. The novel mechanism of IL-15 transfer to the surface of antigen-processing DCs may explain the enhanced potency of IL-15:IL-15Ralpha-coated nanoparticles for antigen delivery. PMID: 26719339
  36. Muscle FNDC5 mRNA expression and irisin release are not IL-15-dependent in mice. PMID: 25920499
  37. Virtual memory T cells develop and mediate bystander protective immunity in an IL-15-dependent manner. PMID: 27097762
  38. Impaired delivery of IL-15 to CD4+ T cells in the colon downmodulates Foxp3 expression and enhances RORgammat expression. CD4+ T cells deprived of IL-15 trigger IBD characterized by production of pro-inflammatory cytokines and accumulation of Th1/Th17 cells. PMID: 26964669
  39. Most (86%) IL15 in serum resides in the free state, with a minor proportion (14%) residing in complex with IL15Ralpha. Results suggest that IL15 is released as a free molecule by an unknown mechanism rather than via cleavage of membrane-bound IL15/IL15Ralpha. PMID: 26812159
  40. IL-15 may influence mononuclear phagocytes subsets in the gut in a novel way that alters the frequency of lamina propria Th17 cells. PMID: 26600079
  41. Downregulation of STAT3 phosphorylation enhances tumoricidal effect of IL-15-activated dendritic cell against doxorubicin-resistant lymphoma and leukemia via TNF-alpha. PMID: 26255115
  42. Exercise-stimulated interleukin-15 is controlled by AMPK and regulates skin metabolism and aging. PMID: 25902870
  43. IL-15 specifically down-regulates RARB expression, and RARB may play a protective role in lung injury caused by smoking or viral infections. PMID: 25923039
  44. Hair follicle expression of IL-15 was required for CD8+ skin-resident memory T (TRM) cells to exert tropism for the epidermis. PMID: 26479922
  45. High levels of IL-15 can promote tumor destruction and reduce metastasis in breast cancer via effects on NK1.1 positive cells. PMID: 25879689
  46. The role of the IL-15 receptor alpha in metabolism and obesity is reported. PMID: 26269523
  47. We demonstrated that IL-15 has a role in mammary cancer immunosurveillance and that IL-15-regulated NK and CD8+ memory cells play a role in long-lasting immunoprevention PMID: 25997501
  48. Type I interferon signaling is required for generating soluble IL-15 complexes. PMID: 25756182
  49. data reveal that inflammatory IL-15 promotes optimal responses by memory CD8+ T cells. PMID: 26241055
  50. IL-15 is involved in adipose tissue regulation and linked to altered mitochondrial function PMID: 25517731

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Subcellular Location Secreted
Protein Families IL-15/IL-21 family
Database Links

KEGG: mmu:16168

STRING: 10090.ENSMUSP00000034148

UniGene: Mm.4392

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