Mouse apolipoprotein H (Apo-H) ELISA Kit

Product Details

Customer Reviews and Q&A

Target Background

Product Details

Target Name

apolipoprotein H (beta-2-glycoprotein I)

Alternative Names

Apoh ELISA kit; B2gp1Beta-2-glycoprotein 1 ELISA kit; APC inhibitor ELISA kit; Activated protein C-binding protein ELISA kit; Apolipoprotein H ELISA kit; Apo-H ELISA kit; Beta-2-glycoprotein I ELISA kit; B2GPI ELISA kit; Beta(2)GPI ELISA kit

Abbreviation

APOH

Uniprot No.

Q01339

Species

Mus musculus (Mouse)

Sample Types

serum, plasma, tissue homogenates

Detection Range

15.6 ng/mL-1000 ng/mL

Sensitivity

3.9 ng/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Cardiovascular

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse Apo-H in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1000	Average %	88
	Range %	83-92
1:2000	Average %	97
	Range %	91-101
1:4000	Average %	89
	Range %	84-92
1:8000	Average %	97
	Range %	91-101

Recovery

The recovery of mouse Apo-H spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	87-95
EDTA plasma (n=4)	103	98-105

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
1000	2.669	2.625	2.647	2.512
500	2.180	2.112	2.146	2.011
250	1.611	1.600	1.606	1.471
125	0.859	0.831	0.845	0.710
62.5	0.515	0.527	0.521	0.386
31.2	0.321	0.333	0.327	0.192
15.6	0.248	0.242	0.245	0.110
0	0.136	0.134	0.135

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx

Description

This Mouse APOH ELISA Kit was designed for the quantitative measurement of Mouse APOH protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 15.6 ng/mL-1000 ng/mL and the sensitivity is 3.9 ng/mL.

Target Background

Function
(From Uniprot)

Binds to various kinds of negatively charged substances such as heparin, phospholipids, and dextran sulfate. May prevent activation of the intrinsic blood coagulation cascade by binding to phospholipids on the surface of damaged cells.

Gene References into Functions

During the parasite's hepatic replication, the C-terminal region of EXP-1 binds ApoH. This molecular interaction plays a pivotal role for successful Plasmodium liver-stage development. Down-regulation of ApoH expression in either hepatic cells or mouse livers by RNA interference resulted in impaired intrahepatic development. PMID: 28137845
A1-A1, which is a soluble analog of ApoER2 that binds pathological beta2GPI/anti-beta2GPI complexes, has a positive impact on hemodynamics in lupus-prone mice with spontaneous anti-beta2GPI antibodies and hypertension PMID: 26990752
These results suggested that CRP/oxidized low density lipoprotein/beta 2-Glycoprotein I complexes may induce lipid accumulation and inflammation in macrophages via the p38/MAPK and NFkappaB signaling pathways. PMID: 27512978
TLR2 increase the stimulating effect of beta2GP1/anti-beta2GP1 complex on the expression of TNF-alpha in mouse peritoneal macrophages PMID: 27053607
The Fifth Domain of Beta 2 Glycoprotein I Protects from Natural IgM Mediated Cardiac Ischaemia Reperfusion Injury. PMID: 27031114
These results indicate that both NF-kappaB and c-Jun/AP-1 are involved in regulating anti-beta2GPI-induced expression of prothrombotic and proinflammatory molecules in vivo. PMID: 26829121
Phospholipid scramblase 1 regulates phosphatidylserine exposure in response to oxygen stress, leading to beta2-glycoprotein I and IgM binding and lipid-mediated, inflammatory responses. PMID: 26216936
beta2-GPI-D-I-tDCs may offer a novel approach for developing therapy for APS patients PMID: 24972993
Data suggest that factors enabling a beta2-glycoprotein I (beta2GPI)-reactive T cell response may predispose individuals to the development of systemic lupus erythematosus (SLE)-related autoantibodies PMID: 25555913
Deletion of beta2 GPI accelerates and potentiates the autoimmune phenotype in male BXSB.Yaa mice PMID: 24692206
beta2-glycoprotein I-derived peptides attenuate injury and inflammation when administered in a therapeutic manner in intestinal ischemia/reperfusion injury. PMID: 23034168
Interaction of beta2-glycoprotein I with lipopolysaccharide leads to Toll-like receptor 4 (TLR4)-dependent activation of macrophages PMID: 21965665
beta2GPI binding to EC requires priming with pro-inflammatory factors which is not needed for uterine and placental localization probably dependent on hormonal changes. PMID: 21791419
gene knock-out mice display enhanced B16 melanoma development and tumor microvessel density PMID: 20655705
Endothelial cell incubation with anti-lysobisphosphatidic-acid MoAb or with patients' IgG resulted in antibody binding to late endosomes and caused beta2GPI redistribution and accumulation in perinuclear vesicular structures reminiscent of late endosomes. PMID: 12008959
Data are consistent with pathogenic mechanisms in antiphospholipid syndrome involving both neutralization of beta(2)-glycoprotein I (beta(2)GPI) function and beta(2)GPI-immunoglobulin complex formation. PMID: 15100383
beta-2-glycoprotein 1 plays an important physiologic role in the recognition of dying cells PMID: 15843904
certain subpopulations of antibodies raised against tetanus toxoid and cross-reactive with beta(2)GPI, because of the molecular mimicry mechanism, could have pathologic potential PMID: 19086991
These results are compatible with a role for apolipoproteins in lipid metabolism and transport in the developing lung in association with the sex difference in surfactant lipid synthesis. PMID: 19106236

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Subcellular Location

Secreted.

Tissue Specificity

Expressed by the liver and secreted in plasma.

Database Links

KEGG: mmu:11818

STRING: 10090.ENSMUSP00000000049

UniGene: Mm.2266

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Code	CSB-E10074m
Size	96T,5×96T,10×96T
Price	Request a Quote or Start an on-line Chat
Trial Size	24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.