Mouse apolipoprotein H (Apo-H) ELISA Kit

Code CSB-E10074m
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Description

This Mouse APOH ELISA Kit was designed for the quantitative measurement of Mouse APOH protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 15.6 ng/mL-1000 ng/mL and the sensitivity is 3.9 ng/mL.

Target Name apolipoprotein H (beta-2-glycoprotein I)
Alternative Names Apoh ELISA kit; B2gp1Beta-2-glycoprotein 1 ELISA kit; APC inhibitor ELISA kit; Activated protein C-binding protein ELISA kit; Apolipoprotein H ELISA kit; Apo-H ELISA kit; Beta-2-glycoprotein I ELISA kit; B2GPI ELISA kit; Beta(2)GPI ELISA kit
Abbreviation APOH
Uniprot No. Q01339
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates
Detection Range 15.6 ng/mL-1000 ng/mL
Sensitivity 3.9 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cardiovascular
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse Apo-H in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1000Average %88
Range %83-92
1:2000Average %97
Range %91-101
1:4000Average %89
Range %84-92
1:8000Average %97
Range %91-101
Recovery
The recovery of mouse Apo-H spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9287-95
EDTA plasma (n=4)10398-105
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
10002.669 2.625 2.647 2.512
5002.180 2.112 2.146 2.011
2501.611 1.600 1.606 1.471
1250.859 0.831 0.845 0.710
62.50.515 0.527 0.521 0.386
31.20.321 0.333 0.327 0.192
15.60.248 0.242 0.245 0.110
00.136 0.134 0.135
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Background

Function
(From Uniprot)
Binds to various kinds of negatively charged substances such as heparin, phospholipids, and dextran sulfate. May prevent activation of the intrinsic blood coagulation cascade by binding to phospholipids on the surface of damaged cells.
Gene References into Functions
  1. During the parasite's hepatic replication, the C-terminal region of EXP-1 binds ApoH. This molecular interaction plays a pivotal role for successful Plasmodium liver-stage development. Down-regulation of ApoH expression in either hepatic cells or mouse livers by RNA interference resulted in impaired intrahepatic development. PMID: 28137845
  2. A1-A1, which is a soluble analog of ApoER2 that binds pathological beta2GPI/anti-beta2GPI complexes, has a positive impact on hemodynamics in lupus-prone mice with spontaneous anti-beta2GPI antibodies and hypertension PMID: 26990752
  3. These results suggested that CRP/oxidized low density lipoprotein/beta 2-Glycoprotein I complexes may induce lipid accumulation and inflammation in macrophages via the p38/MAPK and NFkappaB signaling pathways. PMID: 27512978
  4. TLR2 increase the stimulating effect of beta2GP1/anti-beta2GP1 complex on the expression of TNF-alpha in mouse peritoneal macrophages PMID: 27053607
  5. The Fifth Domain of Beta 2 Glycoprotein I Protects from Natural IgM Mediated Cardiac Ischaemia Reperfusion Injury. PMID: 27031114
  6. These results indicate that both NF-kappaB and c-Jun/AP-1 are involved in regulating anti-beta2GPI-induced expression of prothrombotic and proinflammatory molecules in vivo. PMID: 26829121
  7. Phospholipid scramblase 1 regulates phosphatidylserine exposure in response to oxygen stress, leading to beta2-glycoprotein I and IgM binding and lipid-mediated, inflammatory responses. PMID: 26216936
  8. beta2-GPI-D-I-tDCs may offer a novel approach for developing therapy for APS patients PMID: 24972993
  9. Data suggest that factors enabling a beta2-glycoprotein I (beta2GPI)-reactive T cell response may predispose individuals to the development of systemic lupus erythematosus (SLE)-related autoantibodies PMID: 25555913
  10. Deletion of beta2 GPI accelerates and potentiates the autoimmune phenotype in male BXSB.Yaa mice PMID: 24692206
  11. beta2-glycoprotein I-derived peptides attenuate injury and inflammation when administered in a therapeutic manner in intestinal ischemia/reperfusion injury. PMID: 23034168
  12. Interaction of beta2-glycoprotein I with lipopolysaccharide leads to Toll-like receptor 4 (TLR4)-dependent activation of macrophages PMID: 21965665
  13. beta2GPI binding to EC requires priming with pro-inflammatory factors which is not needed for uterine and placental localization probably dependent on hormonal changes. PMID: 21791419
  14. gene knock-out mice display enhanced B16 melanoma development and tumor microvessel density PMID: 20655705
  15. Endothelial cell incubation with anti-lysobisphosphatidic-acid MoAb or with patients' IgG resulted in antibody binding to late endosomes and caused beta2GPI redistribution and accumulation in perinuclear vesicular structures reminiscent of late endosomes. PMID: 12008959
  16. Data are consistent with pathogenic mechanisms in antiphospholipid syndrome involving both neutralization of beta(2)-glycoprotein I (beta(2)GPI) function and beta(2)GPI-immunoglobulin complex formation. PMID: 15100383
  17. beta-2-glycoprotein 1 plays an important physiologic role in the recognition of dying cells PMID: 15843904
  18. certain subpopulations of antibodies raised against tetanus toxoid and cross-reactive with beta(2)GPI, because of the molecular mimicry mechanism, could have pathologic potential PMID: 19086991
  19. These results are compatible with a role for apolipoproteins in lipid metabolism and transport in the developing lung in association with the sex difference in surfactant lipid synthesis. PMID: 19106236

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Subcellular Location Secreted.
Tissue Specificity Expressed by the liver and secreted in plasma.
Database Links

KEGG: mmu:11818

STRING: 10090.ENSMUSP00000000049

UniGene: Mm.2266

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