Mouse pigment epithelium-derived factor,PEDF ELISA Kit

Code CSB-E08820m
Size 96T,5×96T,10×96T
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Product Details

Target Name
serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 1
Alternative Names
Serpinf1 ELISA Kit; Pedf ELISA Kit; Sdf3Pigment epithelium-derived factor ELISA Kit; PEDF ELISA Kit; Caspin ELISA Kit; Serpin F1 ELISA Kit; Stromal cell-derived factor 3 ELISA Kit; SDF-3 ELISA Kit
Abbreviation
Uniprot No.
Species
Mus musculus (Mouse)
Sample Types
serum, plasma, tissue homogenates, cell culture supernates
Detection Range
15.6 pg/mL-1000 pg/mL
Sensitivity
3.9 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Others
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse PEDF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %85
Range %80-90
1:2Average %95
Range %90-100
1:4Average %94
Range %88-99
1:8Average %94
Range %90-100
Recovery
The recovery of mouse PEDF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9590-102
EDTA plasma (n=4)9992-104
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
10002.334 2.217 2.276 2.129
5001.987 1.891 1.939 1.792
2501.436 1.414 1.425 1.278
1251.005 0.926 0.966 0.819
62.50.718 0.698 0.708 0.561
31.20.422 0.402 0.412 0.265
15.60.203 0.201 0.202 0.055
00.151 0.143 0.147  
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Mouse SERPINF1 ELISA Kit was designed for the quantitative measurement of Mouse SERPINF1 protein in serum, plasma, tissue homogenates, cell culture supernates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL.

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 Q&A
Q:

In regards to the Cusabio product CSB-E08820m, can you please send the target sequence that the antibodies recognise?
Very much appreciated.

A:
Thanks for your inquiry. There are 2 antibodies used in the kit CSB-E08820m. Pls check the detailed information:
Capture antibody: Goat polyclonal antibody
Detection antibody: Goat polyclonal antibody
Sorry we didn't detect the epitope mapping on the antibodies before, so we don't know the recognition area.
The sequence information cannot be provided.Hope you could understand.
Q:

Could you please provide which hosts (mouse or rabbit) and clonalilty (monoclonal or polyclonal) the two abs (capture and detection ab) provided in this kit have?

A:
Thanks for your inquiry.
Pls check the antibody information:
Capture antibody: Goat polyclonal antibody(precoated in the plate already).
Detection antibody: Goat polyclonal antibody(There is the component as Biotin-antibody (100 x concentrate in the kit).

Target Background

Function
(From Uniprot)
Neurotrophic protein; induces extensive neuronal differentiation in retinoblastoma cells. Potent inhibitor of angiogenesis. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity.
Gene References into Functions
  1. PN-1 and PEDF share structural and functional features, and expression patterns in the retina. PMID: 28706437
  2. The findings indicate that PEDF functions as a tumor-suppressor gene in the occurrence of epithelial-mesenchymal transition and metastasis in nasopharyngeal carcinoma. PMID: 28569772
  3. Expression of GLUT1 is stimulated by hyperglycemia and low oxygen supply, and this overexpression was associated with increased activity of GLUT1 in the cell membrane that contributes to the impairment of the RPE secretory function of PEDF. PMID: 27440994
  4. Combination of neuroprotective factors Pedf plus docosahexaenoic acid (DHA) stimulates corneal nerve regeneration. Data suggest activation of Pedfr by Pedf (here, applied topically to treat corneal nerve injury) promotes regeneration of corneal nerve via up-regulation of release of DHA (here, also applied topically) and expression of specific nerve tissue/eye proteins. (Pedfr = pigment epithelium-derived factor receptor) PMID: 28972155
  5. The renoprotective effects of PEDF are mediated, at least partially, by inhibition of the Wnt pathway in kidneys. PMID: 27914705
  6. PEDF deficiency has a significant impact on retinal endothelial cell adhesion and migration through altered production of extracellular matrix and junctional proteins in response to increased oxidative stress affecting their proangiogenic activity. PMID: 28747334
  7. PEDF is a hormone-regulated negative autocrine mediator of endometrial proliferation. PMID: 28911166
  8. These results indicate that PEDF counters Wnt signaling to allow for osteoblast differentiation and provides a mechanistic insight into how the PEDF null state results in OI type VI. PMID: 27127101
  9. Furthermore, pigment epithelium-derived factor (PEDF), a secreted glycoprotein known for its anti-tumor properties, blocked Wnt3a-directed induction of autophagy proteins. Autophagy inhibition was complemented by reciprocal regulation of the oxidative stress enzymes, superoxide dismutase 2 (SOD2) and catalase. PMID: 27557659
  10. Study demonstrated the inhibitory effect of PEDF on insulin-dependent molecular mechanisms of glucose homeostasis, and suggests that PEDF could be a specific target in the management of metabolic disorders. PMID: 26700654
  11. Alpha-2-Antiplasmin mediated myofibroblast formation and the development of renal fibrosis in unilateral ureteral obstruction via induction of TGF-beta. PMID: 25095732
  12. PEDF inhibits retinal microvascular dysfunction induced by 12/15-lipoxygenase-derived eicosanoids in diabetic retinopathy. PMID: 25562624
  13. Data suggest that pigment epithelium-derived factor (PEDF) and adipose triglyceride lipase (ATGL) may serve as therapeutic targets for managing vascular hyperpermeability in sepsis. PMID: 25700221
  14. describe alterations in the PEDF signaling pathway and subventricular zone stem cell self-renewal at a very young age, which could be involved in the premature senescence observed in the SAM-P8 model PMID: 25636741
  15. The combination of rAAV-PEDF with hyperthermia has synergistic therapeutic effects on established solid tumors, with no side effects. PMID: 25003563
  16. PEDF mediates reduced vasculogenesis. PMID: 25054647
  17. The results demonstrated that PEDF can protect 661W cells against light-induced cell death and suggest that the binding of PEDF to cell surface PEDF-R triggers a prosurvival signaling pathway. PMID: 24664775
  18. data suggest that the PEDF expression makes significant contribution to modulation of the inflammatory and angiogenic phenotype of the lung endothelium PMID: 24318110
  19. PEDF suppresses IL-1beta-mediated JNK activation to improve hepatocyte insulin signaling PMID: 24456163
  20. Data suggest that Pedf (pigment epithelium-derived factor) is secreted from 3T3-L1 adipocytes, after treatment of the cell line with HDL (high density lipoprotein). PMID: 23600479
  21. Elevated PEDF may contribute to progressive obesity and insulin resistance via its dual regulation of ATGL. PMID: 23850519
  22. These results suggest that proteinase 3 is new essential regulator of neuronal cell death pathway in a condition of excess neutrophil encounter in neuroinflammatory conditions. PMID: 23748041
  23. PEDF deficiency in mice results in increased total body fat and decreased bone mineralization. PMID: 23887690
  24. Continuous infusion of P78-PEDF for 6 wk resulted in protection from diabetic neuropathy as indicated by reduced albuminuria and blood urea nitrogen, and reduced histological changes compared with vehicle-treated diabetic mice. PMID: 23884140
  25. results suggest that PEDF is crucially involved in the neuroprotective process of reactive Muller cells towards RGC PMID: 22410737
  26. alpha-2 antiplasmin induces dendritic growth in hippocampal neurons through p38 MAPK activation, independent of plasmin PMID: 23646899
  27. the dynamic expression of PEDF that inversely portrays VEGF expression may imply its putative role as a physiological negative regulator of follicular angiogenesis. PMID: 23075882
  28. PEDF could block the AGE-induced apoptotic cell death of podocytes by suppressing RAGE expression and subsequent ROS generation partly via PPARgamma activation PMID: 23108227
  29. PEDF secreted by adipocytes contributes to the onset and maintenance of chronic inflammation in obesity. PMID: 22714715
  30. In a mouse model of ovarian hyperstimulation syndrome, PEDF was nearly undetectable in ovarian granulosa cells. PMID: 23295464
  31. EL-Kras(G12D)/PEDF deficient mice developed invasive pancreatic ductal adenocarcinoma with increased peripancreatic fat with adipocyte hypertrophy and intrapancreatic adipocyte infiltration. PMID: 22234980
  32. These studies are the first to report oligodendroglial morphogenic, survival, and remyelination-enhancing effects of PEDF. PMID: 22933798
  33. a novel mechanism for the tumoricidal activity of PEDF, which involves tumor cell killing via PPARgamma-mediated TRAIL induction in macrophages. PMID: 21846721
  34. Results suggest that PEDF is an endogenous antagonist of LRP6, and blocking Wnt signaling may represent a novel mechanism for its protective effects against diabetic retinopathy. PMID: 21576363
  35. Pigment epithelium-derived factor (PEDF) shares binding sites in collagen with heparin/heparan sulfate proteoglycans. PMID: 21652703
  36. Data indicate that bone marrow stem cells play an important role in regulating neovascularization, and that the ratio of VEGF and PEDF may be an indicator of the pro- or anti-angiogenic activities of BMSCs. PMID: 21236558
  37. Reduced pigment epithelium-derived factor is associated with liver cirrhosis and fibrosis. PMID: 20709803
  38. VEGF/PEDF ratio change was correlated with the development and progression of retinal neovascularization. PMID: 19115638
  39. Erk5 and its activating kinase MEK5 are the upstream mediators of the anti-angiogenic signal by the natural angiogenesis inhibitor, pigment epithelial-derived factor (PEDF). PMID: 20185831
  40. Lentivirus-mediated gene transfer of PEDF decreased the growth of ocular melanoma and its hepatic micrometastasis in a mouse ocular melanoma model. PMID: 19661223
  41. expression by adeno-associated virus type 2 reduces retinal neovascularization PMID: 12072560
  42. mortality due to an experimental acute myocardial infarction was increased in mice deficient in alpha2-antiplasmin but not in mice deficient in other components acting in fibrinolysis PMID: 12239160
  43. Results identify the angiogenesis inhibitor pigment epithelium-derived factor (PEDF) as a key inhibitor of stromal vasculature and epithelial tissue growth in mouse prostate and pancreas. PMID: 12740569
  44. Pedf was down-regulated by ras and neu oncogenes. PMID: 12912908
  45. alpha2-AP is not important to net glomerular fibrin deposition, crescent formation or renal impairment in crescentic glomerulonephritis. PMID: 12941042
  46. PEDF may play a regulatory role during endochondral bone formation and skeletal development in mice. PMID: 15549636
  47. PEDF downregulates Fyn through Fes, resulting in inhibition of FGF-2-induced capillary morphogenesis of endothelial cells PMID: 15713745
  48. lack of alpha(2)AP markedly causes an over-release of VEGF from the fibroblasts in cutaneous wound lesions, thereby inducing angiogenesis around the area, and thus resulting in an accelerated-wound closure PMID: 16839360
  49. These results demonstrate that PEDF could inhibit the cold injury-induced brain edema by blocking the VEGF signaling to hyperpermeability through the suppression of NADPH oxidase via inhibition of Rac-1 activation. PMID: 17692294
  50. GPR39 protects from cell death by increasing secretion of pigment epithelium-derived growth factor PMID: 18180304

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Subcellular Location
Secreted. Melanosome.
Protein Families
Serpin family
Tissue Specificity
Highly expressed in the liver, gastric glandular mucosa and renal tubules. It is also expressed in the brain, heart, lung retina and testes.
Database Links
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