SARS-CoV-2 N ELISA Kit

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Protocol MSDS

Code	CSB-EL33251
Size	96T,5×96T,10×96T
Price	Request a Quote
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Product Details
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Target Background

Product Details

Target Name

Novel Coronavirus Nucleoprotein (SARS-CoV-2 N)

Alternative Names

N; Nucleoprotein; N; Nucleocapsid protein; NC; Protein N

Abbreviation

SARS-CoV-2 N

Uniprot No.

P0DTC9

Sample Types

serum, plasma, swabs

Detection Range

3.12 ng/mL-200 ng/mL

Sensitivity

0.78 ng/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Infectious Diseases

Assay Principle

quantitative

Measurement

Sandwich

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean(ng/ml)	25.39	24.46	26.31	26.65	26.31	24.19
SD	0.027	0.025	0.031	0.041	0.035	0.037
CV(%)	5.7	5.46	6.33	8.26	7.13	8.01

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
200	3.228	3.104	3.166	3.032
100	2.045	2.169	2.107	1.973
50	1.001	0.931	0.966	0.832
25	0.469	0.507	0.488	0.354
12.5	0.268	0.276	0.272	0.138
6.25	0.196	0.204	0.200	0.066
3.12	0.168	0.161	0.165	0.031
0	0.136	0.132	0.134

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.

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Target Background

Function
(From Uniprot)

Packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication. May modulate transforming growth factor-beta signaling by binding host smad3.

Subcellular Location

Virion. Host cytoplasm.

Protein Families

Betacoronavirus nucleocapsid protein family

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