SARS-CoV-2 N ELISA Kit

Code CSB-EL33251
Size 96T,5×96T,10×96T How to order?
Trial Size 24T ELISA kits trial application
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Product Details

Target Name Novel Coronavirus Nucleoprotein (SARS-CoV-2 N)
Alternative Names N; Nucleoprotein; N; Nucleocapsid protein; NC; Protein N
Abbreviation SARS-CoV-2 N
Uniprot No. P0DTC9
Sample Types serum, plasma, swabs
Detection Range 3.12 ng/mL-200 ng/mL
Sensitivity 0.78 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Infectious Diseases
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%  
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%  
Three samples of known concentration were tested in twenty assays to assess.

  Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean(ng/ml) 25.39 24.46 26.31 26.65 26.31 24.19
SD 0.027 0.025 0.031 0.041 0.035 0.037
CV(%) 5.7 5.46 6.33 8.26 7.13 8.01
Typical Data These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml OD1 OD2 Average Corrected
200 3.228 3.104 3.166 3.032
100 2.045 2.169 2.107 1.973
50 1.001 0.931 0.966 0.832
25 0.469 0.507 0.488 0.354
12.5 0.268 0.276 0.272 0.138
6.25 0.196 0.204 0.200 0.066
3.12 0.168 0.161 0.165 0.031
0 0.136 0.132 0.134  
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Background

Function
(From Uniprot)
Packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication. May modulate transforming growth factor-beta signaling by binding host smad3.
Subcellular Location Virion. Host cytoplasm.
Protein Families Betacoronavirus nucleocapsid protein family
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