| Target Name |
Novel Coronavirus Nucleoprotein (SARS-CoV-2 N) |
| Alternative Names |
N; Nucleoprotein; N; Nucleocapsid protein; NC; Protein N |
| Abbreviation |
SARS-CoV-2 N |
| Uniprot No. |
P0DTC9 |
| Sample Types |
serum, plasma, swabs |
| Detection Range |
3.12 ng/mL-200 ng/mL |
| Sensitivity |
0.78 ng/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Infectious Diseases
|
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV%<8% |
|
| Three samples of known concentration were tested twenty times on one plate to assess. |
| Inter-assay Precision (Precision between assays): CV%<10% |
|
| Three samples of known concentration were tested in twenty assays to assess. |
| |
Intra-Assay Precision |
Inter-Assay Precision |
| Sample |
1 |
2 |
3 |
1 |
2 |
3 |
| n |
20 |
20 |
20 |
20 |
20 |
20 |
| Mean(ng/ml) |
25.39 |
24.46 |
26.31 |
26.65 |
26.31 |
24.19 |
| SD |
0.027 |
0.025 |
0.031 |
0.041 |
0.035 |
0.037 |
| CV(%) |
5.7 |
5.46 |
6.33 |
8.26 |
7.13 |
8.01 |
|
| Typical Data |
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
| 200 |
3.228 |
3.104 |
3.166 |
3.032 |
| 100 |
2.045 |
2.169 |
2.107 |
1.973 |
| 50 |
1.001 |
0.931 |
0.966 |
0.832 |
| 25 |
0.469 |
0.507 |
0.488 |
0.354 |
| 12.5 |
0.268 |
0.276 |
0.272 |
0.138 |
| 6.25 |
0.196 |
0.204 |
0.200 |
0.066 |
| 3.12 |
0.168 |
0.161 |
0.165 |
0.031 |
| 0 |
0.136 |
0.132 |
0.134 |
|
|
| ELISA Data Analysis |
Watch ELISA data processing video & download Curve Expert if needed |
Troubleshooting
and FAQs |
ELISA kit FAQs |
| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
