N Recombinant Monoclonal Antibody

^{Rare Species}

Datasheet

Code	CSB-RA33255A0GMY
Size	US$320
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Image	The Binding Activity of SARS-CoV-2-N Antibody with SARS-CoV-2-N Activity: Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-N (CSB-EP3325GMY) at 2 μg/ml can bind SARS-CoV-2-N Antibody, the EC₅₀ is 6.892 ng/ml. In the Colloidal Gold Immunochromatography Assay detection system, the background of antibody (CSB-RA33255A0GMY) is clean, the detection limit can be as low as 446.4ng/ml (31.25ng/0.07ml), and the sensitivity is very good. ELISA: Immobilize various types of SARS proteins at concentration of 2μg/ml on solid substrate, then react with SARS-CoV-2-N Antibody at concentration of 100μg/ml, 10μg/ml and 1μg/ml. It shows the SARS-CoV-2-N Antibody (CSB-RA33255A0GMY) is specific for SARS-CoV-2-N protein, without any cross-reactivity with NL63-CoV, HCoV-OC43, HCoV-229E or HCoV-HKU1.
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Product Details
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Target Background

Product Details

Uniprot No.

P0DTC9

Target Names

N (Nucleoprotein)

Alternative Names

N; Nucleoprotein; N; Nucleocapsid protein; NC; Protein N

Species Reactivity

Human Novel Coronavirus (SARS-CoV-2/ 2019-nCoV)

Immunogen

Recombinant Human Novel Coronavirus Nucleoprotein (N) (1-419aa)(CSB-EP3325GMY)

Immunogen Species

Human Novel Coronavirus (SARS-CoV-2/ 2019-nCoV)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Monoclonal mouse (varialbe region)/human (kappa/IgG1 constant)chimeric antibody

Clone No.

1A6

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, GICA

Recommended Dilution

Application	Recommended Dilution
ELISA	1:10000-1:50000
GICA	1:500-1:2000

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

The SARS-CoV-2 nucleocapsid protein serves as one of the most abundant viral proteins during infection, playing essential roles in viral RNA packaging, replication, and host cell interactions. Its high immunogenicity and conservation make it a primary target for diagnostic development and fundamental coronavirus research, complementing spike protein-focused studies with insights into viral assembly and pathogenesis.

This recombinant monoclonal antibody targeting the SARS-CoV-2 nucleocapsid protein offers the reproducibility and consistency that diagnostic and research applications demand. As a chimeric construct combining mouse variable regions with human kappa and IgG1 constant domains, it provides sequence-defined performance that eliminates the lot-to-lot variability inherent in traditional hybridoma production. The antibody was raised against a recombinant nucleoprotein construct spanning amino acids 1-419, ensuring recognition of properly folded epitopes.

Validation data demonstrate exceptional performance characteristics across immunoassay platforms. In functional ELISA, the antibody achieves an EC50 of 6.892 ng/ml, indicating high-affinity binding suitable for sensitive detection work. The recommended ELISA dilution range of 1:10000 to 1:50000 reflects this potency. For rapid testing applications, colloidal gold immunochromatography assay validation shows clean background signal with detection limits reaching 446.4 ng/ml, supporting point-of-care diagnostic development. Critically, specificity testing confirms no cross-reactivity with nucleocapsid proteins from related human coronaviruses including NL63-CoV, HCoV-OC43, HCoV-229E, and HCoV-HKU1, ensuring reliable discrimination of SARS-CoV-2 in samples where multiple coronavirus exposures may be relevant.

This antibody supports researchers developing SARS-CoV-2 diagnostic platforms, studying viral protein biology, or requiring a reliable detection reagent for nucleocapsid-focused coronavirus investigations.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication. May modulate transforming growth factor-beta signaling by binding host smad3.

Subcellular Location

Virion. Host cytoplasm.

Protein Families

Betacoronavirus nucleocapsid protein family

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Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Related pathways

Virus Infection Target Therapy

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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