The recombinant N antibody was prepared by obtaining the antibody genes, cloning the genes into a plasma vector to construct vector clone, transfecting the vector clone into a mammalian cell line for transient expression, and purifying the antibody by affinity-chromatography. This recombinant N antibody has been verified to detect the N protein from Human Novel Coronavirus (SARS-CoV-2/ 2019-nCoV) in the ELISA, GICA.
SARS-CoV-2 N protein, a highly immunogenic viral protein, is encoded by the ninth ORF of the virus. It is involved in viral assembly, replication, and host immune response regulation. The N protein recognizes and binds to RNA, allowing for genome encapsidation and participating in the assembly of the virion through its interactions with the membrane protein M. It is an immunodominant antigen in host immune responses that can be used as a diagnostic antigen and immunogen. The N protein is more sensitive than the S protein in response to adaptive immunity, making it a better indicator of early disease.