N Antibody

Code CSB-RA33255A0GMY
Size US$520 How to order?
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  • The Binding Activity of SARS-CoV-2-N Antibody with SARS-CoV-2-N
    Activity: Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-N (CSB-EP3325GMY) at 2 μg/ml can bind SARS-CoV-2-N Antibody, the EC50 is 6.892 ng/ml.

  • In the Colloidal Gold Immunochromatography Assay
    detection system, the background of antibody (CSB-RA33255A0GMY) is clean, the detection limit can be as low as 446.4ng/ml (31.25ng/0.07ml), and the sensitivity is very good.

  • ELISA: Immobilize various types of SARS proteins at concentration of 2μg/ml on solid substrate, then react with SARS-CoV-2-N Antibody at concentration of 100μg/ml, 10μg/ml and 1μg/ml. It shows the SARS-CoV-2-N Antibody (CSB-RA33255A0GMY) is specific for SARS-CoV-2-N protein, without any cross-reactivity with NL63-CoV, HCoV-OC43, HCoV-229E or HCoV-HKU1.
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Product Details

Description

The recombinant N antibody was prepared by obtaining the antibody genes, cloning the genes into a plasma vector to construct vector clone, transfecting the vector clone into a mammalian cell line for transient expression, and purifying the antibody by affinity-chromatography. This recombinant N antibody has been verified to detect the N protein from Human Novel Coronavirus (SARS-CoV-2/ 2019-nCoV) in the ELISA, GICA.

SARS-CoV-2 N protein, a highly immunogenic viral protein, is encoded by the ninth ORF of the virus. It is involved in viral assembly, replication, and host immune response regulation. The N protein recognizes and binds to RNA, allowing for genome encapsidation and participating in the assembly of the virion through its interactions with the membrane protein M. It is an immunodominant antigen in host immune responses that can be used as a diagnostic antigen and immunogen. The N protein is more sensitive than the S protein in response to adaptive immunity, making it a better indicator of early disease.

Uniprot No. P0DTC9
Target Names N (Nucleoprotein)
Alternative Names N; Nucleoprotein; N; Nucleocapsid protein; NC; Protein N
Species Reactivity Human Novel Coronavirus (SARS-CoV-2/ 2019-nCoV)
Immunogen Recombinant Human Novel Coronavirus Nucleoprotein (N) (1-419aa)(CSB-EP3325GMY)
Immunogen Species Human Novel Coronavirus (SARS-CoV-2/ 2019-nCoV)
Conjugate Non-conjugated
Clonality Monoclonal
Isotype Monoclonal mouse (varialbe region)/human (kappa/IgG1 constant)chimeric antibody
Clone No. 1A6
Purification Method Affinity-chromatography
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form Liquid
Tested Applications ELISA, GICA
Recommended Dilution
Application Recommended Dilution
ELISA 1:10000-1:50000
GICA 1:500-1:2000
Protocols ELISA Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Background

Function
Packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication. May modulate transforming growth factor-beta signaling by binding host smad3.
Subcellular Location Virion. Host cytoplasm.
Protein Families Betacoronavirus nucleocapsid protein family
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Address
7505 Fannin St. Ste 610-312, Houston, TX 77054, USA
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