N Recombinant Monoclonal Antibody

^{Rare Species}

Datasheet

Code	CSB-RA33255A1GMY
Size	US$210
	Order now
Image	Western Blot Positive WB detected in: His tag-tagged SARS-CoV-2 nucleocapsid recombinant protein from E. Coli (CSB-EP3325GMY) SARS-CoV-2 nucleocapsid antibody at 1:1000 (CSB-RA33255A1GMY) Secondary Peroxidase-Affinipure Goat Anti-Human IgG Fc Fragment Specific at 1/20000 dilution Predicted band size: 48 kDa Observed band size: 55 kDa The Binding Activity of SARS-CoV-2-N Antibody with SARS-CoV-2-N Activity: Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-N at 2 μg/ml can bind SARS-CoV-2-N Antibody, the EC₅₀ is 1.586 ng/ml. In the Colloidal Gold Immunochromatography Assay detection system, the background of antibody (CSB-RA33255A1GMY) is clean, the detection limit can be as low as 125ng/ml (8.75ng/0.07ml), and the sensitivity is very good. ELISA: Immobilize various types of SARS proteins at concentration of 2μg/ml on solid substrate, then react with SARS-CoV-2-N Antibody at concentration of 100μg/ml, 10μg/ml and 1μg/ml. It shows the SARS-CoV-2-N Antibody (CSB-RA33255A1GMY) is specific for SARS-CoV-2-N protein, without any cross-reactivity with NL63-CoV, HCoV-OC43, HCoV-229E or HCoV-HKU1. The Binding Activity of N protein with N Recombinant Antibody. Activity: Measured by its binding ability in a functional ELISA. Immobilized N at 2 μg/ml can bind N Recombinant Antibody, the EC₅₀ of N Recombinant Antibody. The Binding Activity of N protein with N Recombinant Antibody. Activity: Measured by its binding ability in a functional ELISA. Immobilized N at 2 μg/ml can bind N Recombinant Antibody, the EC₅₀ of N Recombinant Antibody. The Binding Activity of N protein with N Recombinant Antibody. Activity: Measured by its binding ability in a functional ELISA. Immobilized N at 2 μg/ml can bind N Recombinant Antibody, the EC₅₀ of N Recombinant Antibody.
Have Questions?	Leave a Message or Start an on-line Chat

Product Details
Citations
Related Products
Customer Reviews and Q&A
Target Background

Product Details

Uniprot No.

P0DTC9

Alternative Names

N; Nucleoprotein; N; Nucleocapsid protein; NC; Protein N

Species Reactivity

Human Novel Coronavirus (SARS-CoV-2/ 2019-nCoV)

Immunogen

Recombinant Human Novel Coronavirus Nucleoprotein (N) (1-419aa) (CSB-EP3325GMY)

Immunogen Species

Human Novel Coronavirus (SARS-CoV-2/ 2019-nCoV)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Mouse scFv fusion with human IgG1 Fc

Clone No.

1A6

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB, GICA

Recommended Dilution

Application	Recommended Dilution
ELISA	1:10000-1:50000
WB	1:500-1:5000
GICA	1:1000-1:10000

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
CSB-RA33255A1GMY ELISA protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

The SARS-CoV-2 nucleocapsid protein plays a central role in viral replication and assembly, making it one of the most abundant and immunogenic proteins produced during infection. Its high expression levels and structural conservation have established it as a primary target for diagnostic development and fundamental coronavirus research, enabling detection of viral presence and investigation of host-pathogen interactions.

This recombinant monoclonal antibody targeting the SARS-CoV-2 nucleocapsid protein offers the consistency and reliability that demanding research applications require. As a sequence-defined reagent produced through recombinant technology, it eliminates the lot-to-lot variability inherent in traditional hybridoma-derived antibodies, ensuring your experimental results remain reproducible across studies. The mouse scFv fusion with human IgG1 Fc format provides stable performance while maintaining compatibility with standard secondary detection systems.

Validation data demonstrates exceptional specificity for SARS-CoV-2 nucleocapsid protein without cross-reactivity against related coronavirus proteins including NL63-CoV, HCoV-OC43, HCoV-229E, and HCoV-HKU1, a critical consideration when distinguishing SARS-CoV-2 from endemic human coronaviruses. In Western blot analysis using His-tagged recombinant nucleocapsid protein expressed in E. coli, the antibody detects a band at approximately 55 kDa, slightly higher than the predicted 48 kDa molecular weight, likely reflecting post-translational modifications or anomalous migration patterns common with highly basic proteins like nucleocapsid. Functional ELISA testing reveals strong binding activity with an EC50 of 1.586 ng/ml, while colloidal gold immunochromatography assay validation demonstrates clean background signal with detection sensitivity reaching 125 ng/ml.

This antibody supports researchers developing SARS-CoV-2 diagnostic platforms, studying viral protein biology, or requiring a dependable nucleocapsid detection reagent across ELISA, Western blot, and lateral flow applications.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Citations

High-Sensitivity Detection of Infectious Disease via Fe3O4@UiO-66-NH2 Combined with a Graphene Field-Effect Transistor M Wang, G Zhou, W Hai, Y Zhang,ACS Infectious Diseases,2025

Customer Reviews and Q&A

■ Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Target Background

Function

Packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication. May modulate transforming growth factor-beta signaling by binding host smad3.

Subcellular Location

Virion. Host cytoplasm.

Protein Families

Betacoronavirus nucleocapsid protein family

Promotions

Email: [email protected]
Distributors Worldwide

Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Related pathways

Virus Infection Target Therapy

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

Join us with

Subscribe newsletter

Leave a message

Message

Product >> Product Name：
Product >> Code：
Name： *
Email： *
Company/Organization：
Phone：
Since emails may get flagged by spam filters occasionally, we recommend leaving your phone number as a precaution. Your information is safe with us.
Question/Comment： *
Your Region： *
I'd like to receive email newsletters from Cusabio

Yes

No
How do you know CUSABIO?

Email

Distributor

Search engine

Advertisement

Others
CAPTCHA verification：
Please enter the characters shown. Click the image to refresh if unclear.

Place an order now

I. Product details

Product name *

Code *

Size *

Quantity *

Message

II. Contact details

Name *

Email address *

Company/Organization

Phone

III. Ship To

Address *

City *

State/Province *

Zip/Post code *

Country *

Attn Name *

Phone# *

IV. Bill To Same as Shipping Address