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CCR8｜C5AR1｜CD24｜CLDN18.2｜CD20｜Proteinase K｜EGFR｜PD-L1｜CD276｜Exosome Isolation Kits
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CCR8｜C5AR1｜CD24｜CLDN18.2｜CD20｜Proteinase K｜EGFR｜PD-L1｜CD276｜Exosome Isolation Kits
| Code | CSB-RA33255A1GMY |
| Size |
US$350Purchase it in Cusabio online store (only available for customers from the US) |
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| Description |
Recombinant anti-SARS-CoV-2 Nucleoprotein (N protein) Mouse single-chain variable fragment (ScFv) is expressed from 293 cells (HEK293) with a human IgG1 Fc tag on C-terminal. The resulting product is the recombinant SARS-CoV-2 N monoclonal antibody. This N antibody is recommended for ELISA, WB, and GICA applications. It has undergone affinity-chromatography purification. And it can detect the endogenous content of human SARS-Cov-2 N protein. The SARS-CoV-2 N protein is an abundant RNA-binding protein. It contains two structured domains: the RNA binding domain and the oligomerization domain, which are separated by a long flexible linker. The separated domains allow the N protein to execute many functions in the viral lifecycle such as RNA replication, virion assembly, and immune system interference. |
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| Uniprot No. | P0DTC9 | ||||||||
| Alternative Names | Nucleocapsid protein, NC, protein N, N | ||||||||
| Species Reactivity | Human Novel Coronavirus (SARS-CoV-2/ 2019-nCoV) | ||||||||
| Immunogen | Recombinant Human Novel Coronavirus Nucleoprotein (N) (1-419aa) (CSB-EP3325GMY) | ||||||||
| Immunogen Species | Human Novel Coronavirus (SARS-CoV-2/ 2019-nCoV) | ||||||||
| Conjugate | Non-conjugated | ||||||||
| Clonality | Monoclonal | ||||||||
| Isotype | Mouse scFv fusion with human IgG1 Fc | ||||||||
| Clone No. | 1A6 | ||||||||
| Purification Method | Affinity-chromatography | ||||||||
| Concentration | It differs from different batches. Please contact us to confirm it. | ||||||||
| Buffer | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
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| Form | Liquid | ||||||||
| Tested Applications | ELISA, WB, GICA | ||||||||
| Recommended Dilution |
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| Protocols | ELISA Protocol Western Blotting(WB) Protocol CSB-RA33255A1GMY ELISA protocol |
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| Troubleshooting and FAQs | Antibody FAQs | ||||||||
| Storage | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. | ||||||||
| Lead Time | Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time. |
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| Function (From Uniprot) |
Nucleoprotein packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. It plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication. Coronavirus nucleoproteins are phosphoproteins, and are encoded near the 3′ end of the genome. N possesses two RNA-binding domains: an N-terminal domain with adjacent S/R-rich motif and the C-terminal 209 amino acids. N protein is invovled in coronavirus infection with many ways: the C-terminal domain (CTD) of N is important for binding the genomic RNA packaging signal leading to selective genome incorporation, the N3 domain interacts with the endodomain of M to form virions, and the serine–arginine repeat region of N (SR) interacts with the first ubiquitin-like domain of nsp3 in a critical early replication step. Moreover, it has also been demonstrated that N can oligomerize through interactions in the CTD, bind viral RNA through the N-terminal domain, unwind double-stranded nucleic acid in the manner of an RNA chaperone, and pack in a helix through the N-terminal domain, though none of these other functions has yet been demonstrated to be important for infection.
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| Subcellular Location | Virion i UniRule annotation, Host endoplasmic reticulum-Golgi intermediate compartment i UniRule annotation, Host Golgi apparatus |
| Protein Families | Betacoronavirus nucleocapsid protein family |
