SARS-CoV-2 N Antibody Pair 1

Code CSB-EAP33255A1
Size US$1000Purchase it in Cusabio online store
(only available for customers from the US)
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  • CSB-EAP33255A1 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). An antibody specific for SARS-CoV-2 Nucleoprotein (N) has been pre-coated onto the microwells. The SARS-CoV-2 Nucleoprotein (N) protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody HRP conjugated specific for SARS-CoV-2 Nucleoprotein (N) is added to detect the captured SARS-CoV-2 Nucleoprotein (N) protein. Followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm.
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Product Details

Uniprot No. P0DTC9
Alternative Names Nucleocapsid protein, NC, protein N, N
Species Reactivity Human Novel Coronavirus (SARS-CoV-2/ 2019-nCoV)
Immunogen Recombinant Human Novel Coronavirus Nucleoprotein (N) (1-419aa)
Immunogen Species Human Novel Coronavirus (SARS-CoV-2/ 2019-nCoV)
Concentration It differs from different batches. Please contact us to confirm it.
Form Liquid
Protein Names Human Novel Coronavirus Nucleoprotein (N)
Host Capture: Mouse scFv fusion with human IgG1 Fc
Detection: Mouse
Buffer Capture: 50% Glycerol, 0.01M PBS, PH 7.4
Detection: 50% Glycerol, 0.01M PBS, PH 7.4
Components Capture: CSB-EAP33255A1C
Detection: CSB-EAP33255A1B(HRP)
Reagents are sufficient for at least 5 x 96 well plates using recommended protocol.
Tested Applications S-ELISA
Troubleshooting and FAQs Antibody FAQs
Notes We recommend using the capture antibody at a concentration of 1ug/ml and the detection antibody at a concentration of 0.42ug/ml. Optimal dilutions should be determined experimentally by the researcher.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Background

Function
(From Uniprot)
Nucleoprotein packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. It plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication. Coronavirus nucleoproteins are phosphoproteins, and are encoded near the 3′ end of the genome. N possesses two RNA-binding domains: an N-terminal domain with adjacent S/R-rich motif and the C-terminal 209 amino acids. N protein is invovled in coronavirus infection with many ways: the C-terminal domain (CTD) of N is important for binding the genomic RNA packaging signal leading to selective genome incorporation, the N3 domain interacts with the endodomain of M to form virions, and the serine–arginine repeat region of N (SR) interacts with the first ubiquitin-like domain of nsp3 in a critical early replication step. Moreover, it has also been demonstrated that N can oligomerize through interactions in the CTD, bind viral RNA through the N-terminal domain, unwind double-stranded nucleic acid in the manner of an RNA chaperone, and pack in a helix through the N-terminal domain, though none of these other functions has yet been demonstrated to be important for infection.
Subcellular Location Virion i UniRule annotation, Host endoplasmic reticulum-Golgi intermediate compartment i UniRule annotation, Host Golgi apparatus
Protein Families Betacoronavirus nucleocapsid protein family

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