N Antibody

Code CSB-PA33254A0Rb
Size US$299Purchase it in Cusabio online store
(only available for customers from the US)
Image
  •  The Binding Activity of SARS-CoV-2-N Antibody with SARS-CoV-2-N
    Activity: Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-N (CSB-EP3325GMY) at 2 μg/ml can bind SARS-CoV-2-N Antibody, the EC50 is 201.1 ng/ml.
  • Western Blot
    Positive WB detected in: SARS-CoV-2 nucleocapsid recombinant protein from E. Coli (CSB-EP3325GMY1) at 100ng
    All lanes N antibody at 1:2000
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 18 KDa
    Observed band size: 18 KDa
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Product Details

Description

CUSABIO uses the full-length amino acid sequence of the recombinant human SARS-CoV-2 nucleoprotein (N) to immunize rabbits thus obtaining the N antibody (CSB-PA33254A0Rb). This N antibody is a polyclonal antibody and occurs as an unconjugated IgG. Its purity is greater than 95% using protein G purified. It only reacts with human SARS-CoV-2 N protein. It has been validated for use in ELISA and WB applications. In the functional ELISA, this N antibody can bind to the SARS-CoV-2 N protein, and its EC50 is 201.1 ng/ml. The target protein SARS-CoV-2 N is highly expressed within infected cells and is essential for viral RNA replication and assembling into new virions.

Uniprot No. P0DTC9
Alternative Names N; Nucleoprotein; N; Nucleocapsid protein; NC; Protein N
Raised in Rabbit
Species Reactivity Human Novel Coronavirus (SARS-CoV-2/ 2019-nCoV)
Immunogen Recombinant Human Novel Coronavirus Nucleoprotein (N) (1-419aa) (CSB-EP3325GMY)
Immunogen Species Human Novel Coronavirus (SARS-CoV-2/ 2019-nCoV)
Conjugate Non-conjugated

The N Antibody (Product code: CSB-PA33254A0Rb) is Non-conjugated. For N Antibody with conjugates, please check the following table.

Available Conjugates
Conjugate Product Code Product Name Application
HRP CSB-PA33254B0Rb N Antibody, HRP conjugated ELISA
FITC CSB-PA33254C0Rb N Antibody, FITC conjugated
Biotin CSB-PA33254D0Rb N Antibody, Biotin conjugated ELISA
Clonality Polyclonal
Isotype IgG
Purification Method >95%, Protein G purified
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form Liquid
Tested Applications WB, ELISA
Recommended Dilution
Application Recommended Dilution
WB 1000-5000
ELISA 1:1000-1:5000
Protocols Western Blotting(WB) Protocol
ELISA Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Background

Function
Packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication. May modulate transforming growth factor-beta signaling by binding host smad3.
Subcellular Location Virion. Host cytoplasm.
Protein Families Betacoronavirus nucleocapsid protein family
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