N Antibody

Code CSB-PA33254A0Rb
Size US$166
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  •  The Binding Activity of SARS-CoV-2-N Antibody with SARS-CoV-2-N
    Activity: Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-N (CSB-EP3325GMY) at 2 μg/ml can bind SARS-CoV-2-N Antibody, the EC50 is 201.1 ng/ml.
  • Western Blot
    Positive WB detected in: SARS-CoV-2 nucleocapsid recombinant protein from E. Coli (CSB-EP3325GMY1) at 100ng
    All lanes N antibody at 1:2000
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 18 KDa
    Observed band size: 18 KDa
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Product Details

Uniprot No.
Alternative Names
N; Nucleoprotein; N; Nucleocapsid protein; NC; Protein N
Raised in
Rabbit
Species Reactivity
Human Novel Coronavirus (SARS-CoV-2/ 2019-nCoV)
Immunogen
Recombinant Human Novel Coronavirus Nucleoprotein (N) (1-419aa) (CSB-EP3325GMY)
Immunogen Species
Human Novel Coronavirus (SARS-CoV-2/ 2019-nCoV)
Conjugate
Non-conjugated

The N Antibody (Product code: CSB-PA33254A0Rb) is Non-conjugated. For N Antibody with conjugates, please check the following table.

Available Conjugates
Conjugate Product Code Product Name Application
HRP CSB-PA33254B0Rb N Antibody, HRP conjugated ELISA
FITC CSB-PA33254C0Rb N Antibody, FITC conjugated
Biotin CSB-PA33254D0Rb N Antibody, Biotin conjugated ELISA
Clonality
Polyclonal
Isotype
IgG
Purification Method
>95%, Protein G purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
WB, ELISA
Recommended Dilution
Application Recommended Dilution
WB 1000-5000
ELISA 1:1000-1:5000
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Description

CUSABIO uses the full-length amino acid sequence of the recombinant human SARS-CoV-2 nucleoprotein (N) to immunize rabbits thus obtaining the N antibody (CSB-PA33254A0Rb). This N antibody is a polyclonal antibody and occurs as an unconjugated IgG. Its purity is greater than 95% using protein G purified. It only reacts with human SARS-CoV-2 N protein. It has been validated for use in ELISA and WB applications. In the functional ELISA, this N antibody can bind to the SARS-CoV-2 N protein, and its EC50 is 201.1 ng/ml. The target protein SARS-CoV-2 N is highly expressed within infected cells and is essential for viral RNA replication and assembling into new virions.

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Target Background

Function
Packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication. May modulate transforming growth factor-beta signaling by binding host smad3.
Subcellular Location
Virion. Host cytoplasm.
Protein Families
Betacoronavirus nucleocapsid protein family
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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