N Antibody

Datasheet

Code	CSB-PA33254A0Rb
Size	US$166
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Image	The Binding Activity of SARS-CoV-2-N Antibody with SARS-CoV-2-N Activity: Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-N (CSB-EP3325GMY) at 2 μg/ml can bind SARS-CoV-2-N Antibody, the EC₅₀ is 201.1 ng/ml. Western Blot Positive WB detected in: SARS-CoV-2 nucleocapsid recombinant protein from E. Coli (CSB-EP3325GMY1) at 100ng All lanes N antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 18 KDa Observed band size: 18 KDa
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Target Background

Product Details

Uniprot No.

P0DTC9

Alternative Names

N; Nucleoprotein; N; Nucleocapsid protein; NC; Protein N

Raised in

Rabbit

Species Reactivity

Human Novel Coronavirus (SARS-CoV-2/ 2019-nCoV)

Immunogen

Recombinant Human Novel Coronavirus Nucleoprotein (N) (1-419aa) (CSB-EP3325GMY)

Immunogen Species

Human Novel Coronavirus (SARS-CoV-2/ 2019-nCoV)

Conjugate

Non-conjugated

The N Antibody (Product code: CSB-PA33254A0Rb) is Non-conjugated. For N Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA33254B0Rb	N Antibody, HRP conjugated	ELISA
FITC	CSB-PA33254C0Rb	N Antibody, FITC conjugated
Biotin	CSB-PA33254D0Rb	N Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

WB, ELISA

Recommended Dilution

Application	Recommended Dilution
WB	1000-5000
ELISA	1:1000-1:5000

Protocols

Western Blotting(WB) Protocol
ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

CUSABIO uses the full-length amino acid sequence of the recombinant human SARS-CoV-2 nucleoprotein (N) to immunize rabbits thus obtaining the N antibody (CSB-PA33254A0Rb). This N antibody is a polyclonal antibody and occurs as an unconjugated IgG. Its purity is greater than 95% using protein G purified. It only reacts with human SARS-CoV-2 N protein. It has been validated for use in ELISA and WB applications. In the functional ELISA, this N antibody can bind to the SARS-CoV-2 N protein, and its EC50 is 201.1 ng/ml. The target protein SARS-CoV-2 N is highly expressed within infected cells and is essential for viral RNA replication and assembling into new virions.

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Target Background

Function

Packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication. May modulate transforming growth factor-beta signaling by binding host smad3.

Subcellular Location

Virion. Host cytoplasm.

Protein Families

Betacoronavirus nucleocapsid protein family

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Related pathways

Virus Infection Target Therapy

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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