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| Code | CSB-PA33254A0Rb |
| Size | US$299 How to order? |
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| Description |
CUSABIO uses the full-length amino acid sequence of the recombinant human SARS-CoV-2 nucleoprotein (N) to immunize rabbits thus obtaining the N antibody (CSB-PA33254A0Rb). This N antibody is a polyclonal antibody and occurs as an unconjugated IgG. Its purity is greater than 95% using protein G purified. It only reacts with human SARS-CoV-2 N protein. It has been validated for use in ELISA and WB applications. In the functional ELISA, this N antibody can bind to the SARS-CoV-2 N protein, and its EC50 is 201.1 ng/ml. The target protein SARS-CoV-2 N is highly expressed within infected cells and is essential for viral RNA replication and assembling into new virions. |
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| Uniprot No. | P0DTC9 | ||||||
| Alternative Names | N; Nucleoprotein; N; Nucleocapsid protein; NC; Protein N | ||||||
| Raised in | Rabbit | ||||||
| Species Reactivity | Human Novel Coronavirus (SARS-CoV-2/ 2019-nCoV) | ||||||
| Immunogen | Recombinant Human Novel Coronavirus Nucleoprotein (N) (1-419aa) (CSB-EP3325GMY) | ||||||
| Immunogen Species | Human Novel Coronavirus (SARS-CoV-2/ 2019-nCoV) | ||||||
| Conjugate | Non-conjugated The N Antibody (Product code: CSB-PA33254A0Rb) is Non-conjugated. For N Antibody with conjugates, please check the following table. |
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| Clonality | Polyclonal | ||||||
| Isotype | IgG | ||||||
| Purification Method | >95%, Protein G purified | ||||||
| Concentration | It differs from different batches. Please contact us to confirm it. | ||||||
| Buffer | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
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| Form | Liquid | ||||||
| Tested Applications | WB, ELISA | ||||||
| Recommended Dilution |
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| Protocols | Western Blotting(WB) Protocol ELISA Protocol |
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| Troubleshooting and FAQs | Antibody FAQs | ||||||
| Storage | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. | ||||||
| Lead Time | Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time. |
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| Function |
Packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication. May modulate transforming growth factor-beta signaling by binding host smad3.
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| Subcellular Location | Virion. Host cytoplasm. |
| Protein Families | Betacoronavirus nucleocapsid protein family |
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