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- A 96-well Coated assay plate --This microplate has been pre-coated with human SARS-CoV-2 nucleoprotein.
- Negative Control (1 x 800 μl) --It is free of the SARS-CoV-2 N IgG antibody and used to preclude the false positive.
- Positive Control (1 x 800 μl) --Used to evaluate the validity, stability, and comparability of the test results.
- HRP-conjugated anti-Human IgG antibody (100 x concentrate) (1 x 120 μl) --Act as the detection antibody.
- HRP-conjugate Diluent (1 x 20 ml) --Dilute the HRP-conjugated anti-Human IgG antibody.
- Sample Diluent (2 x 20 ml) --Dilute the sample solution.
- Wash Buffer (25 x concentrate) (1 x 20 ml) --Wash the unbound reagents.
- TMB Substrate (1 x 10 ml) --React with HRP, eliciting a chromogenic color reaction.
- Stop Solution (1 x 10 ml) --Stop the color reaction. The solution turns from blue to yellow.
- Four Adhesive Strips (For 96 wells) --Seal the microplate when incubation.
- An Instruction manual
