Pig Progonadoliberin-2(GNRH2) ELISA kit

Code CSB-EL009636PI
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Lead Time 7-14 working days
Abbreviation GNRH2
Target Name gonadotropin-releasing hormone 2
Alias GnRH-II, LH-RHII, OTTHUMP00000030076|OTTHUMP00000030077|luliberin II|luteinizing hormone-releasing hormone II|progonadoliberin II
Species Sus scrofa (Pig)
Sample Types serum, plasma, tissue homogenates
Detection Range 40 pg/mL-8000 pg/mL
Sensitivity 20 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Competitive
Target Details This protein is a preproprotein that is cleaved to form a secreted 10 aa peptide hormone. The secreted decapeptide regulates reproduction in females by stimulating the secretion of both luteinizing- and follicle-stimulating hormones. Three transcript variants that encode unique proproteins but the same peptide hormone have been found for this gene.
HGNC 4420
Precision
Intra-assay Precision (Precision within an assay): CV%<15%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<15%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of pig GNRH2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %87
Range %81-92
1:2Average %96
Range %90-105
1:4Average %97
Range %92-105
1:8Average %94
Range %88-103
Recovery
The recovery of pig GNRH2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9590-99
EDTA plasma (n=4)9789-105
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2Average
80000.186 0.189 0.188
20000.321 0.325 0.323
6400.520 0.518 0.519
1600.811 0.807 0.809
401.321 1.326 1.324
01.967 1.988 1.978
References
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