Pig Progonadoliberin-2(GNRH2) ELISA kit

Code CSB-EL009636PI
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name gonadotropin-releasing hormone 2
Alternative Names GnRH-II, LH-RHII, OTTHUMP00000030076|OTTHUMP00000030077|luliberin II|luteinizing hormone-releasing hormone II|progonadoliberin II
Abbreviation GNRH2
Species Sus scrofa (Pig)
Sample Types serum, plasma, tissue homogenates
Detection Range 40 pg/mL-8000 pg/mL
Sensitivity 20 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Competitive
Intra-assay Precision (Precision within an assay): CV%<15%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<15%
Three samples of known concentration were tested in twenty assays to assess.
To assess the linearity of the assay, samples were spiked with high concentrations of pig GNRH2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
1:1Average %87
Range %81-92
1:2Average %96
Range %90-105
1:4Average %97
Range %92-105
1:8Average %94
Range %88-103
The recovery of pig GNRH2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9590-99
EDTA plasma (n=4)9789-105
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
80000.186 0.189 0.188
20000.321 0.325 0.323
6400.520 0.518 0.519
1600.811 0.807 0.809
401.321 1.326 1.324
01.967 1.988 1.978
and FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days

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