Pig Progonadoliberin-2(GNRH2) ELISA kit

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Pig Progonadoliberin-2(GNRH2) ELISA kit

Citations (2) Protocol

Code	CSB-EL009636PI
Size	96T,5×96T,10×96T
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Product Details
Citations (2)
Customer Reviews and Q&A

Target Name

gonadotropin-releasing hormone 2

Alternative Names

GnRH-II, LH-RHII, OTTHUMP00000030076|OTTHUMP00000030077|luliberin II|luteinizing hormone-releasing hormone II|progonadoliberin II

Abbreviation

GNRH2

Species

Sus scrofa (Pig)

Sample Types

serum, plasma, tissue homogenates

Detection Range

40 pg/mL-8000 pg/mL

Sensitivity

20 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Signal Transduction

Assay Principle

quantitative

Measurement

Competitive

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of pig GNRH2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	87
	Range %	81-92
1:2	Average %	96
	Range %	90-105
1:4	Average %	97
	Range %	92-105
1:8	Average %	94
	Range %	88-103

Recovery

The recovery of pig GNRH2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	90-99
EDTA plasma (n=4)	97	89-105

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average
8000	0.186	0.189	0.188
2000	0.321	0.325	0.323
640	0.520	0.518	0.519
160	0.811	0.807	0.809
40	1.321	1.326	1.324
0	1.967	1.988	1.978

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.

Description

This Pig GNRH2 ELISA Kit was designed for the quantitative measurement of Pig GNRH2 protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 40 pg/mL-8000 pg/mL and the sensitivity is 20 pg/mL.

Renal Tubular DamageMay Contribute More to Acute Hyperglycemia Induced Kidney Injury in Non-diabetic Conscious Rats Jing-Yu Wang et al,ScienceDirect,2015
LH-Independent Testosterone Secretion Is Mediated by the Interaction Between GNRH2 and Its Receptor Within Porcine Testes Desaulniers AT. et al,Biol Reprod.,2015

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

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