Rat Interleukin 18,IL-18 Elisa Kit

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Target Data

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Rat Interleukin 18,IL-18 Elisa Kit

Name: Rat Interleukin 18,IL-18 Elisa Kit
Brand: CUSABIO
SKU: CSB-E04610r

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Code	CSB-E04610r
Size	96T,5×96T,10×96T
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Target Data

Target Name

interleukin 18 (interferon-gamma-inducing factor)

Alternative Names

IGIF, IL-18, IL-1g, IL1F4, MGC12320, IL-1 gamma|interferon-gamma-inducing factor|interleukin 18|interleukin-1 gamma|interleukin-18

Abbreviation

IL18

Uniprot No.

P97636

Species

Rattus norvegicus (Rat)

Sample Types

serum, plasma, cell culture supernates

Detection Range

0.156 ng/mL-10 ng/mL

Sensitivity

0.039 ng/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat IL-18 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	96
	Range %	90-102
1:2	Average %	89
	Range %	84-95
1:4	Average %	100
	Range %	95-106
1:8	Average %	97
	Range %	92-104

Recovery

The recovery of rat IL-18 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	93-96
EDTA plasma (n=4)	102	98-107

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
10	2.357	2.387	2.372	2.218
5	1.686	1.699	1.693	1.539
2.5	1.064	1.015	1.040	0.886
1.25	0.687	0.654	0.671	0.517
0.625	0.382	0.398	0.390	0.236
0.312	0.289	0.270	0.280	0.126
0.156	0.221	0.235	0.228	0.074
0	0.151	0.157	0.154

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days

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Function	Augments natural killer cell activity in spleen cells and stimulates interferon gamma production in T-helper type I cells.
Subcellular Location	Secreted
Protein Families	IL-1 family
Database Links	KEGG: rno:29197 STRING: 10116.ENSRNOP00000013093 UniGene: Rn.11118

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

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