Rat Interleukin 18,IL-18 Elisa Kit

Code CSB-E04610r
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P97636
Lead Time 3-5 working days
Abbreviation IL18
Target Name interleukin 18 (interferon-gamma-inducing factor)
Alias IGIF, IL-18, IL-1g, IL1F4, MGC12320, IL-1 gamma|interferon-gamma-inducing factor|interleukin 18|interleukin-1 gamma|interleukin-18
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, cell culture supernates
Detection Range 0.156 ng/mL-10 ng/mL
Sensitivity 0.039 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details This protein is a proinflammatory cytokine. This cytokine can induce the IFN-gamma production of T cells. The combination of this cytokine and IL12 has been shown to inhibit IL4 dependent IgE and IgG1 production, and enhance IgG2a production of B cells. IL-18 binding protein (IL18BP) can specifically interact with this cytokine, and thus negatively regulate its biological activity.
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat IL-18 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 96  
Range % 90-102  
1:2 Average % 89  
Range % 84-95  
1:4 Average % 100  
Range % 95-106  
1:8 Average % 97  
Range % 92-104  
Recovery
The recovery of rat IL-18 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 89 93-96  
EDTA plasma (n=4) 102 98-107  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected  
10 2.357 2.387 2.372 2.218  
5 1.686 1.699 1.693 1.539  
2.5 1.064 1.015 1.040 0.886  
1.25 0.687 0.654 0.671 0.517  
0.625 0.382 0.398 0.390 0.236  
0.312 0.289 0.270 0.280 0.126  
0.156 0.221 0.235 0.228 0.074  
0 0.151 0.157 0.154    
References
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Function Augments natural killer cell activity in spleen cells and stimulates interferon gamma production in T-helper type I cells.
Subcellular Location Secreted
Protein Families IL-1 family
Database Links

KEGG: rno:29197

STRING: 10116.ENSRNOP00000013093

UniGene: Rn.11118

Pathway Inflammasome Signaling
NOD-like receptor signaling pathway

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