Rat Interleukin 18,IL-18 Elisa Kit

Code CSB-E04610r
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name interleukin 18 (interferon-gamma-inducing factor)
Alternative Names Il18 ELISA Kit; Igif ELISA Kit; Interleukin-18 ELISA Kit; IL-18 ELISA Kit; Interferon gamma-inducing factor ELISA Kit; IFN-gamma-inducing factor ELISA Kit; Interleukin-1 gamma ELISA Kit; IL-1 gamma ELISA Kit
Abbreviation IL18
Uniprot No. P97636
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, cell culture supernates
Detection Range 0.156 ng/mL-10 ng/mL
Sensitivity 0.039 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
To assess the linearity of the assay, samples were spiked with high concentrations of rat IL-18 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 96  
Range % 90-102  
1:2 Average % 89  
Range % 84-95  
1:4 Average % 100  
Range % 95-106  
1:8 Average % 97  
Range % 92-104  
The recovery of rat IL-18 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 89 93-96  
EDTA plasma (n=4) 102 98-107  
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected  
10 2.357 2.387 2.372 2.218  
5 1.686 1.699 1.693 1.539  
2.5 1.064 1.015 1.040 0.886  
1.25 0.687 0.654 0.671 0.517  
0.625 0.382 0.398 0.390 0.236  
0.312 0.289 0.270 0.280 0.126  
0.156 0.221 0.235 0.228 0.074  
0 0.151 0.157 0.154    
and FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days


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Target Data

Function Augments natural killer cell activity in spleen cells and stimulates interferon gamma production in T-helper type I cells.
Gene References into Functions
  1. IL18 and the related markers were closely associated with the occurrence and development of deep venous thrombosis. PMID: 29786104
  2. These observations suggest that IL-18 exerts direct effects upon the GnRH neuron via IL-18Ralpha and acts on GnRH neurons through an autocrine or paracrine pathway. PMID: 28373090
  3. These findings also indicate that IL-18 is an important regulator leading to MMP-13 expression and cell migration in astrocytes. PMID: 26558633
  4. These results suggest that the expression of interferon-gamma via IL-18 in the lenses of OVX rats is induced by ovariectomy PMID: 26725437
  5. Excessive IL-18 Relate the Aggravation of Indomethacin-Induced Intestinal Ulcerogenic Lesions in Adjuvant-Induced Arthritis Rat PMID: 26424019
  6. results provided evidence that delivery of IL-18 and IFN-beta by BMSCs may be an excellent and promising approach to develop an effective treatment protocol for glioma therapy. PMID: 26252165
  7. PMA treatment during a vulnerable period can alter brain development. IL-18 and IRAK-4 appear to be important for the development of PMA induced injury. PMID: 25918710
  8. Remifentanil protects the liver against I/R injury by modulating hepatic IL-18/IL-18BP balance and inhibiting IL-18 signaling PMID: 25919765
  9. The results in vitro showed the protective effect of IL-18 on cortical neurons. PMID: 25022959
  10. Using Dynamic Bayesian Network inference, we identified interleukin-18 as a central node associated with neuropathic pain in chronic sciatic constriction injury in rats. PMID: 26004155
  11. IL-18 can augment the hepatocyte growth ability via NF-kappaB and p38/ATF2 by targeting cyclin B1, cyclin B2, cyclin A2 and Bcl-2 in BRL-3A rat liver cells. PMID: 25752290
  12. IL-18 serves as a positive regulator of hepatocyte proliferation. PMID: 24412291
  13. IL18 was significantly increased in rat benign prostatic hyperplasia tissue. PMID: 24615654
  14. During learning, changes in IL-18 are restricted to the dorsal hippocampus. PMID: 23747799
  15. Changes in macrophage IL-18 and vascular fibrinogen deopsition following injury in aging rats contribute to local inflammation and postinjury neointima formation. PMID: 24414074
  16. interferon-gamma increases NF-kappaB and cytokine IL-18, but decreases IL-27 in acute pancreatitis PMID: 23725508
  17. IFN-gamma production via IL-18 in the retinas of 60-week-old OLETF rats is caused by hyperglycemia, and plays a role in the inflammation of the OLETF rat retinas. PMID: 23823918
  18. Chronic exposure of Sprague-Dawley rats to second hand smoke results in a significant increase of proinflammatory cytokine IL-18 in the bronchoalveolar lavage fluid. PMID: 23392573
  19. IL-18 expression increases in brain following chronic neuroinflammation elicited by systemic inflammation. PMID: 22642744
  20. Il-18-mediated up-regulation plays a role in maintenance of blood brain barrier function following status elepticus. PMID: 22338606
  21. Our findings are the first report that injury of trigeminal nerve induced IL-18 upregulation in activated microglia in the Vc, suggesting a possible role of IL-18 in orofacial neuropathic pain. PMID: 23000553
  22. Serum IL-18 level increased in the process of deep vein thrombosis. PMID: 22318348
  23. Suggest that the angiotensin II/AT1/Nox1/IL-18 pathway is a critical factor in hyperplastic vascular diseases. PMID: 22636674
  24. The treatment of rats with anti-IL-18 antibody at the time of burn injury prevented intestine apoptosis and normalized tight junction proteins following EtOH and burn injury. PMID: 22001439
  25. differential expression in abortive rats as compared to of pregnant and non-pregnant rats PMID: 21920610
  26. These results indicate that IL-18 secreted by microglia, which was activated by C6 glioma-derived ECM, enhanced migration of C6 glioma through NO/cGMP pathway. PMID: 21442623
  27. IL-18 delays neutrophil apoptosis following EtOH and burn injury by modulating the pro- and antiapoptotic proteins. PMID: 20844839
  28. Expression of IL-18, IL-6 and oxidative stress in rat peritoneal mesothelial cells stimulated by lipopolysaccharide PMID: 21882482
  29. The expression of IL-18 was increased in the lenses of OLETF rat. It is possible that activated IL-18 in the lenses of OLETF rat may be related to the lens epithelial cell apotosis and lens opacification. PMID: 21591858
  30. IL-18 may serve as an additional marker to monitor the severity of inflammation during pancreatitis. PMID: 21273803
  31. Transcripts of core components of NOD-like receptor Nlrp6 inflammasome (Nlrp6, Pycard, Caspase-1) and one of its substrates, IL-18, were increased at E20 compared with E16 in fetal intestine and not lung. PMID: 21088234
  32. chronic elevated IL-18 levels at a supraphsiological concentration aggravated insulin resistance, enhanced vascular inflammation and remodeling, probably by increasing the level of IRAK1 and the activity of NF-kappaB PMID: 19717152
  33. Role in acute lung inflammation PMID: 11739527
  34. Up-regulation of IL-18 and IL-12 in the ileum of neonatal rats with necrotizing enterocolitis. PMID: 12032269
  35. IL-18 occured in the medial habenula (MHbS), the interpenducular nucleus, and in the ependymal cells surrounding the third and the lateral ventricles. Restraint stress induced a strong elevation of IL-18 in the MHbS but not in ependymal cells. PMID: 12468024
  36. The frequent expression of IL-18 in thyrocytes suggests that IL-18 itself might be a secreted immunomodulator in autoimmune thyroid disease PMID: 12490070
  37. Translational control of IL-18 expression by its 5'-UTR limits production of IL-18, resulting in restricted expression of mRNA and protein IFN-gamma in this model of crescentic glomerulonephritis(GN). Might amplify CD8+-mediated macrophage-dependent GN. PMID: 12787406
  38. IL-18 induces CXCL16 expression via a MyD88, IRAK1-IRAK4-TRAF6, c-Src, PI3K, Akt, JNK, AP-1 pathway PMID: 15890643
  39. IL-18 may have host-protective and growth-promoting functions in the testis. PMID: 16002206
  40. The combined insult of EtOH and burn injury results in increased CORT levels, which in turn up-regulates intestinal IL-18 levels and thereby causes altered intestinal barrier function following a combined insult of EtOH intoxication and burn injury. PMID: 16707557
  41. These results identify a critical role for IL-18 in neointimal formation in a rat model of vascular injury and suggest a potential role for IL-18 neutralization in the reduction of neointimal development. PMID: 16864728
  42. mRNA and the protein level were attenuated by post hypoxia-ischemia hypothermia and that post hypoxia-ischemia hypothermia may decrease microglia activation in the developing brain. PMID: 17010950
  43. Presence of neutrophils appears to be critical for IL-18-meditaed increased lung tissue edema following a combined insult of ethanol and burn injury. PMID: 17220368
  44. IL-18 plays a role in enhancing the lipopolysaccharide-induced neutrophilic inflammation of the lung, but does not affect the resolution of inflammation. PMID: 17352829
  45. IL-18 is regulated by parathyroid hormone and is required for its bone anabolic actions PMID: 18165223
  46. Augmented IL-18 in serum and cardiac tissue in metabolic syndrome may contribute to the coronary perivascular fibrosis. PMID: 18504504
  47. Rapamycin and Sanglifehrin A, but not Cyclosporin A, block IL-18 production and this novel Rapamycin blockade effect on IL-18 may contribute to the ability of Rapamycin to inhibit chronic allograft nephropathy and restenosis. PMID: 18662782
  48. Our results indicate that IL-18-mediated microglia/astrocyte interactions in the spinal cord have a substantial role in the generation of tactile allodynia. PMID: 19036970
  49. Neutrophil chemokines CINC-1/3 may have a role in IL-18-mediated increase in neutrophil O2- production and intestinal edema following alcohol intoxication and burn injury. PMID: 19497959

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Subcellular Location Secreted
Protein Families IL-1 family
Database Links

KEGG: rno:29197

STRING: 10116.ENSRNOP00000013093

UniGene: Rn.11118


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