24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now
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|Intra-assay Precision (Precision within an assay): CV%<8%|
|Three samples of known concentration were tested twenty times on one plate to assess.|
|Inter-assay Precision (Precision between assays): CV%<10%|
|Three samples of known concentration were tested in twenty assays to assess.|
|To assess the linearity of the assay, samples were spiked with high concentrations of rat IL-18 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.|
|The recovery of rat IL-18 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.|
|Sample Type||Average % Recovery||Range|
|EDTA plasma (n=4)||102||98-107|
|These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.|
The product CSB-E04610r is a ready-to-use microwell, strip plate ELISA kit for quantitatively analyzing the presence of the IL-18 in different biological samples, including serum, plasma, and tissue homogenates. This IL-18 ELISA kit specifically recognizes the rat IL-18 in the sample. It adopts the Sandwich ELISA technique in conjunction with antibody-antigen specific binding and an enzyme colorimetric reaction to indicate the presence of rat IL-18 and quantitatively measure the concentration of IL-18 in the samples.
IL-18 is a proinflammatory cytokine that primarily functions to augment the generation of IFNγ from T and NK cells, especially when IL-12p70 is present. It plays an important role in innate and adaptive immunity. IL-18 also promotes the production of other proinflammatory cytokines such as TNFα, IL-8, and IL-1β, to increase the expansion, migration, and activation of neutrophils during infections. In the central nervous system (CNS), IL-18 is involved in neuroinflammatory/neurodegenerative processes and also influences homeostasis and behavior. IL-18 binds to IL-18Rα and then recruits IL-18Rβ, triggering the binding of MyD88, leading to the phosphorylation of IRAK andTRAF6 and activation of NFκB and MAPK pathways, eventually regulating related inflammatory gene transcription, including Ifng, Tnfa, Cd40l, and FasL.
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