Rat Interleukin 18,IL-18 Elisa Kit

Rat Interleukin 18,IL-18 Elisa Kit

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Target

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Il18 Antibodies

Il18 Proteins

Il18 Antibodies

Il18 Proteins

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ELISA Kit
Rat Interleukin 18,IL-18 Elisa Kit

Specification
Target

Code

CSB-E04610r

See More Details

Product Type

ELISA Kit

Size

96T,5×96T,10×96T

Uniprot No.

P97636

Lead Time

3-5 working days

Abbreviation

IL18

Target Name

interleukin 18 (interferon-gamma-inducing factor)

Alias

IGIF, IL-18, IL-1g, IL1F4, MGC12320, IL-1 gamma|interferon-gamma-inducing factor|interleukin 18|interleukin-1 gamma|interleukin-18

Species

Rattus norvegicus (Rat)

Sample Types

serum, plasma, cell culture supernates

Detection Range

0.156 ng/mL-10 ng/mL

Sensitivity

0.039 ng/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Protocol

Instructions

Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.

Assay Principle

quantitative

Measurement

Sandwich

Target Details

This protein is a proinflammatory cytokine. This cytokine can induce the IFN-gamma production of T cells. The combination of this cytokine and IL12 has been shown to inhibit IL4 dependent IgE and IgG1 production, and enhance IgG2a production of B cells. IL-18 binding protein (IL18BP) can specifically interact with this cytokine, and thus negatively regulate its biological activity.

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat IL-18 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	96
	Range %	90-102
1:2	Average %	89
	Range %	84-95
1:4	Average %	100
	Range %	95-106
1:8	Average %	97
	Range %	92-104

Recovery

The recovery of rat IL-18 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	93-96
EDTA plasma (n=4)	102	98-107

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
10	2.357	2.387	2.372	2.218
5	1.686	1.699	1.693	1.539
2.5	1.064	1.015	1.040	0.886
1.25	0.687	0.654	0.671	0.517
0.625	0.382	0.398	0.390	0.236
0.312	0.289	0.270	0.280	0.126
0.156	0.221	0.235	0.228	0.074
0	0.151	0.157	0.154

References

Protective effect of adrenomedullin on contrast induced nephropathy in rats Salih Inal et al,Nefrología (Madrid),2014 34(6):724-31
Dietary Fructose Activates Insulin Signaling and Inflammation in Adipose Tissue: Modulatory Role of Resveratrol Mehmet Bilgehan Pektas.et al,BioMed Research International,2016 Feb:10
Involvement of S100A8/A9-TLR4-NLRP3 Inflammasome Pathway in Contrast-Induced Acute Kidney Injury Tan X.et al,Cell Physiol Biochem.,Aug 30
Propofol Does Not Reduce Pyroptosis of Enterocytes and Intestinal Epithelial Injury After Lipopolysaccharide Challenge Xu-Yu Zhang.et al,Digestive Diseases and Sciences,Oct 23
Correlation between the expression of IL‑18 and deep venous thrombosis Guangdi Li.et al,International Journal of Molecular Medicine,May 16
Combined ischemic postconditioning and ozone postconditioning provides synergistic protection against renal ischemia and reperfusion injury through inhibiting pyroptosis Wang L, et al,Urology,Octobert 22
Suhuang antitussive capsule inhibits NLRP3 inflammasome activation and ameliorates pulmonary dysfunction via suppression of endoplasmic reticulum stress in cough variant asthma Qin W, et al,Livestock Science,July 14

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Function	Augments natural killer cell activity in spleen cells and stimulates interferon gamma production in T-helper type I cells.
Subcellular Location	Secreted
Protein Families	IL-1 family
Database Links	KEGG: rno:29197 STRING: 10116.ENSRNOP00000013093 UniGene: Rn.11118
Pathway	Inflammasome Signaling NOD-like receptor signaling pathway

Il18 Antibody

Il18 Protein

Il18 Antibodies for Rattus norvegicus (Rat)

Code	Product Name	Species Reactivity	Application
CSB-PA011608XA01RA	IL18 Antibody	Rattus norvegicus (Rat)	ELISA, WB (ensure identification of antigen)

Il18 Proteins for Rattus norvegicus (Rat)

Code	Product Name	Source
CSB-YP011608RA CSB-EP011608RA CSB-BP011608RA CSB-MP011608RA	Recombinant Rat Interleukin-18(Il18)	Yeast E.coli Baculovirus Mammalian cell
CSB-RP176194r	Recombinant Rat Interleukin-18(Il18)	E.coli

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.