Rat Interleukin 18,IL-18 Elisa Kit

Instructions
Code CSB-E04610r
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application

Product Details

Target Name interleukin 18 (interferon-gamma-inducing factor)
Alternative Names IGIF, IL-18, IL-1g, IL1F4, MGC12320, IL-1 gamma|interferon-gamma-inducing factor|interleukin 18|interleukin-1 gamma|interleukin-18
Abbreviation IL18
Uniprot No. P97636
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, cell culture supernates
Detection Range 0.156 ng/mL-10 ng/mL
Sensitivity 0.039 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat IL-18 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 96  
Range % 90-102  
1:2 Average % 89  
Range % 84-95  
1:4 Average % 100  
Range % 95-106  
1:8 Average % 97  
Range % 92-104  
Recovery
The recovery of rat IL-18 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 89 93-96  
EDTA plasma (n=4) 102 98-107  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected  
10 2.357 2.387 2.372 2.218  
5 1.686 1.699 1.693 1.539  
2.5 1.064 1.015 1.040 0.886  
1.25 0.687 0.654 0.671 0.517  
0.625 0.382 0.398 0.390 0.236  
0.312 0.289 0.270 0.280 0.126  
0.156 0.221 0.235 0.228 0.074  
0 0.151 0.157 0.154    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days
Still Have Questions? Leave a Message or Start an on-line Chat

Target Data

Function Augments natural killer cell activity in spleen cells and stimulates interferon gamma production in T-helper type I cells.
Subcellular Location Secreted
Protein Families IL-1 family
Database Links

KEGG: rno:29197

STRING: 10116.ENSRNOP00000013093

UniGene: Rn.11118

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