Rat Nephrin ELISA Kit

Code CSB-E13957r
Size 96T,5×96T,10×96T
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now
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Product Details

Target Name
nephrosis 1, congenital, Finnish type (nephrin)
Alternative Names
Nphs1 ELISA Kit; Nphn ELISA Kit; Nephrin ELISA Kit; Renal glomerulus-specific cell adhesion receptor ELISA Kit
Uniprot No.
Rattus norvegicus (Rat)
Sample Types
serum, plasma, tissue homogenates
Detection Range
1.25 ng/mL-80 ng/mL
0.312 ng/mL
Assay Time
Sample Volume
Detection Wavelength
450 nm
Research Area
Signal Transduction
Assay Principle
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
To assess the linearity of the assay, samples were spiked with high concentrations of rat Nephrin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
1:1Average %91
Range %86-95
1:2Average %95
Range %91-98
1:4Average %95
Range %81-89
1:8Average %100
Range %97-103
The recovery of rat Nephrin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 10197-105
EDTA plasma (n=4)9895-102
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
802.215 2.194 2.205 2.049
401.846 1.804 1.825 1.669
201.444 1.468 1.456 1.300
101.033 1.068 1.051 0.895
50.634 0.671 0.653 0.497
2.50.373 0.398 0.386 0.230
1.250.297 0.298 0.298 0.142
00.154 0.157 0.156
and FAQs
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx

This Rat NPHS1 ELISA Kit was designed for the quantitative measurement of Rat NPHS1 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 1.25 ng/mL-80 ng/mL and the sensitivity is 0.312 ng/mL.

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Target Background

(From Uniprot)
Seems to play a role in the development or function of the kidney glomerular filtration barrier. Regulates glomerular vascular permeability. May anchor the podocyte slit diaphragm to the actin cytoskeleton. Plays a role in skeletal muscle formation through regulation of myoblast fusion.
Gene References into Functions
  1. Data show that metformin provides some protection against podocyte damage in diabetic rats, which may be related, in part, to its effect in reducing urinary nephrin (Nphs1) excretion as well as improving podocyte nephrin expression. PMID: 27248136
  2. The effects of sildenafil citrate on urinary podocin and nephrin mRNA expression in an L-NAME model of pre-eclampsia. PMID: 27995418
  3. Irbesartan treatment ameliorates proteinuria in puromycin aminonucleoside nephropathy by inhibiting the reduction of NEPH1 and nephrin in the kidney glomerulus. PMID: 25298195
  4. These findings suggest that candesartan can prevent cisplatin-induced testicular damage by regulating expression patterns of the nephrin-podocin complex in the testes. PMID: 26539476
  5. Rat nephrin Tyr1127 and Tyr1152 are essential for SHP-1 induced nephrin dephosphorylation under high-glucose conditions. PMID: 25404734
  6. Resveratrol alleviated dyslipidemia and renal damage induced by high-fat diet also reduced TNF-alpha and MCP-1 levels, and reduced expressions of nephrin and podocin. PMID: 25025298
  7. findings show that the filament-forming GTPase septin 7 is expressed in podocytes and associates with CD2-associated protein (CD2AP) and nephrin, both essential for glomerular ultrafiltration PMID: 22809625
  8. Soy protein preserves basement membrane integrity through a synergistic effect on nephrin, matrix metalloproteinase and vascular endothelial growth factor PMID: 22094487
  9. Huaiqihuang Granule can up-regulate the expressions of nephrin and podocin in renal tissues of rats with adriamycin-induced nephrosis. PMID: 21565142
  10. The decrease of nephrin expression and the change of its distribution might be the significant factors resulting in considerable proteinuria. PMID: 20629321
  11. Abnormal expression of nephrin is the important molecular mechanism that leads to the occurrence and development of proteinuria. PMID: 20045917
  12. These observations indicate that podocyte-specific expression of rat nephrin can rescue nephrin-deficient mice from perinatal death, but is not sufficient for full complementation. PMID: 19948823
  13. nephrin anchors the slit diaphragm to the actin cytoskeleton, possibly by linkage to CD2AP PMID: 11880318
  14. reduction in nephrin may be a determinant of glomerular hyperpermeability in diabetic nephropathy PMID: 12105259
  15. in normotensive and hypertensive rats, nephrin expression increased from birth to the adult age and down-regulation of nephrin in SHR evident from the early developmental kidney to adulthood may contribute to the development of albuminuria in adult SHR. PMID: 12109777
  16. Expression of nephrin in proteinuric states and in developing glomeruli. Review. PMID: 12386277
  17. Depletion in glomerular nephrin expression in diabetic animals was only abrogated by valsartan treatment, the therapy which was most effective at retarding the development of albuminuria in this model. PMID: 12544453
  18. Nephrin and Neph1 form a hetero-oligomeric receptor complex in the plane of the membrane that might interact across the foot process intercellular junction through interactions between Nephrin with itself and Neph1 PMID: 12646566
  19. nephrin and Neph1 interact and have roles in glomerular permeability PMID: 12865409
  20. Onset of proteinuria in passive Heymann nephritis model of membranous nephropathy is coincident with complement-dependent alterations in association of nephrin with actin cytoskeleton and loss of podocyte slit diaphragm integrity. PMID: 14633129
  21. Nephrin expression and its distribution were altered in puromycin aminonucleoside nephrosis in rats. PMID: 15385636
  22. Data show that in glomerulonephritis in rats, an upregulation of nephrin expression occurs with a concomitant transient downregulation of podocin and CD2AP which may account for a highly dysregulated filtration barrier and increased proteinuria. PMID: 15465010
  23. NPHN contributes to a porous slit diaphragm scaffold, the molecular filter in renal glomerular capillaries. PMID: 15545998
  24. Fetal membranes and placenta of the rat express mRNA for the protein barriers nephrin and Neph 1, suggesting a role in the regulation of protein transfer from the fetus to mother PMID: 15979540
  25. Nck couples nephrin to the actin cytoskeleton in glomerular podocytes and contributes to the maintenance of normal morphology and function of podocytes PMID: 16934223
  26. Downregulation of glomerular Nephrin gene expression in minimal-change like nephropathy. PMID: 17429054
  27. Darbepoetin treatment ameliorates podocyte injury and decreases proteinuria by a direct effect on podocytes. This may be accomplished by maintenance of the actin cytoskeleton and nephrin expression. PMID: 17457371
  28. the nephrin gene is differentially expressed according to glomerular size PMID: 17599973
  29. PI3K is involved in nephrin-mediated actin reorganization in podocytes. PMID: 18033240
  30. Tyrosine phosphorylation of nephrin by regulating F-actin formation may be important for the maintenance of normal podocyte morphology and function. PMID: 18256598
  31. Neph1 but not nephrin specifically binds to adaptor protein Grb2 and tyrosine kinase Csk in a phosphorylation-dependent manner. PMID: 18258597
  32. PEDF could reduce proteinuria by suppressing podocyte damage and decreased nephrin as well as increased VEGF expression in the glomeruli of nephrotic syndrome model rats. PMID: 19042927
  33. Nck is required to maintain adult podocytes and phosphotyrosine-based interactions with nephrin may occur in foot processes of resting, mature podocytes. PMID: 19443634

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Subcellular Location
Cell membrane; Single-pass type I membrane protein.
Protein Families
Immunoglobulin superfamily
Tissue Specificity
Strongly expressed in the podocytes of kidney glomeruli (at protein level) and at lower levels in the spleen.
Database Links
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