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| Code | CSB-E13399r |
| Size | 96T,5×96T,10×96T |
| Price | Request a Quote or Start an on-line Chat |
| Trial Size |
24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now |
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
| To assess the linearity of the assay, samples were spiked with high concentrations of rat COX-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 98 | ||||
| Range % | 92-106 | |||||
| 1:2 | Average % | 95 | ||||
| Range % | 91-99 | |||||
| 1:4 | Average % | 92 | ||||
| Range % | 88-96 | |||||
| 1:8 | Average % | 85 | ||||
| Range % | 80-89 | |||||
| The recovery of rat COX-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 92 | 88-98 | ||||
| EDTA plasma (n=4) | 94 | 90-98 | ||||
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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The rat COX-2 ELISA Kit quantitates rat COX-2 levels in serum, plasma, and tissue homogenates. COX-2 (PTGS2) is the key rate-limiting enzyme needed for the synthesis of prostanoids, including PGE2, PGD2, PGF2a, PGI2, and TAX2, from an essential fatty acid arachidonic acid (AA). It is constitutively expressed in certain cell groups of the brain and inducibly expressed in response to inflammatory reactions. In the central nervous system (CNS), COX-2 affects memory, sensory integration, and autonomic modulation. COX-2 overexpression is mainly linked to inflammation, apoptosis inhibition, uncontrolled cell proliferation, growth, metastasis, neovascularization, and angiogenesis finally resulting in cancer. COX-2 is often expressed in many malignant tumors and is associated with occurrence, progression, and prognosis.
This kit employs the sandwich-ELISA mechanism in conjugation with COX-2 antibody-COX-2 antigen-specific binding as well as HRP-TMB chromogenic reaction to measure the concentration of COX-2 in the samples. The kit is characterized by high sensitivity, strong specificity, good linearity, high recovery, and a precision of less than 10%.
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How can I convert pyggrams per decilitre to picograms per milligram, because your kits are in deciliters and concentrates
picogeram/dl---picogeram/mg
this kits according to picogerm/dl, i want according to picogeram /mg
