Rat cyclooxygenase-2,COX-2 ELISA Kit

Instructions
Code CSB-E13399r
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase)
Alternative Names Ptgs2 ELISA Kit; Cox-2 ELISA Kit; Cox2 ELISA Kit; Prostaglandin G/H synthase 2 ELISA Kit; EC 1.14.99.1 ELISA Kit; Cyclooxygenase-2 ELISA Kit; COX-2 ELISA Kit; PHS II ELISA Kit; Prostaglandin H2 synthase 2 ELISA Kit; PGH synthase 2 ELISA Kit; PGHS-2 ELISA Kit; Prostaglandin-endoperoxide synthase 2 ELISA Kit
Abbreviation PTGS2
Uniprot No. P35355
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, tissue homogenates
Detection Range 1.56 ng/mL-100 ng/mL
Sensitivity 0.39 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Metabolism
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat COX-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 98  
Range % 92-106  
1:2 Average % 95  
Range % 91-99  
1:4 Average % 92  
Range % 88-96  
1:8 Average % 85  
Range % 80-89  
Recovery
The recovery of rat COX-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 92 88-98  
EDTA plasma (n=4) 94 90-98  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected  
100 2.386 2.285 2.336 2.191  
50 1.628 1.527 1.578 1.433  
25 0.937 0.916 0.927 0.782  
12.5 0.564 0.575 0.570 0.425  
6.25 0.365 0.376 0.371 0.226  
3.12 0.240 0.251 0.246 0.101  
1.56 0.195 0.191 0.193 0.048  
0 0.145 0.144 0.145    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Citations

Customer Reviews and Q&A

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 Q&A
Q:

How can I convert pyggrams per decilitre to picograms per milligram, because your kits are in deciliters and concentrates
picogeram/dl---picogeram/mg
this kits according to picogerm/dl, i want according to picogeram /mg

A:
Thanks for your inquiry. For the kit indicated,convertions are not suggested.We recommand you caculate according to the unit stated in the manual.
Pls let me know if you have any further questions. Thank you.

Target Background

Function
(From Uniprot)
Converts arachidonate to prostaglandin H2 (PGH2), a committed step in prostanoid synthesis. Constitutively expressed in some tissues in physiological conditions, such as the endothelium, kidney and brain, and in pathological conditions, such as in cancer. PTGS2 is responsible for production of inflammatory prostaglandins. Up-regulation of PTGS2 is also associated with increased cell adhesion, phenotypic changes, resistance to apoptosis and tumor angiogenesis. In cancer cells, PTGS2 is a key step in the production of prostaglandin E2 (PGE2), which plays important roles in modulating motility, proliferation and resistance to apoptosis.
Gene References into Functions
  1. Increased expression of COX-2 is associated with aortic endothelial dysfunction. PMID: 30292829
  2. findings indicated that BMP9 is involved in the phosphate-induced calcification in VSMCs and COX-2 partly mediates the BMP9-induced calcification in VSMCs through activating Wnt/beta-catenin pathway. PMID: 29073723
  3. the cortical COX2 pathway was activated in CUMS rats. Inhibition of COX2 activity/expression can obviously improve depressive behaviours in CUMS rats. Upregulating COX2 expression can increase the susceptibility of rats to CUMS. PMID: 28352129
  4. The present study proposed that COX-2-mediated inflammation is important as a candidate target for hippocampal impairment in the hypothyroidism. PMID: 29436670
  5. COX1 and 2 gene expression and localization in the injured brain regulating prostaglandin synthase. PMID: 28933223
  6. In heart irradiation groups, 3-fold up-regulation of both ET-1 and COX-2 was observed. In pelvis irradiation groups, 3-fold up-regulation of ET-1 was seen, but not significant changes in COX-2 gene expression have observed at distant heart tissues after pelvis irradiation. PMID: 28508833
  7. Microglial TNFalpha induces cyclooxygenase 2 (COX2) and PGI2 synthase expression in spinal endothelial cells during neuropathic pain. These findings further suggest that the glia-endothelial cell interaction of the neurovascular unit via transient TNFalpha is involved in the generation of neuropathic pain. PMID: 28451639
  8. The authors measured brain oxygenation in localized areas from freely-moving rodents and discovered a severe hypoxic event (pO2 < 10 mmHg) after the termination of seizures. This event lasted over an hour, is mediated by hypoperfusion, generalizes to people with epilepsy, and is attenuated by inhibiting cyclooxygenase-2 or L-type calcium channels. PMID: 27874832
  9. C2-ceramide enhances phenylephrine-induced vascular smooth muscle constriction by mediation of the ER stress/COX-2/PGE2 pathway. PMID: 28797762
  10. Results point to an excess of reactive oxygen species mainly from NAD(P)H oxidase after aluminum exposure and increased vascular prostanoids from COX-2 acting in concert to decrease NO bioavailability, thus inducing vascular dysfunction and increasing blood pressure. PMID: 28826906
  11. Hepatic COX2 expression and PGI2 production are enhanced in cirrhotic rats, but the correlation with Hepatic encephalopathy (HE) is not remarkable. PMID: 27580512
  12. a stiffness-dependent YAP/TAZ-mediated positive feedback loop that drives remodeling phenotypes in pulmonary artery smooth muscle cells via reduced COX-2 and prostaglandin activity. PMID: 28642262
  13. the disrupted pattern of hippocampal neurogenesis induced by MNU is different between developmental and postpubertal exposure and is dependant on COX2 regulation. PMID: 28688903
  14. the long-lasting oligemia following cortical spreading depression consists of at least two distinct temporal phases, mediated by preferential actions of COX-1- and COX-2-derived prostanoids: an initial phase mediated by COX-1 that involves TXA2 followed by a later phase, mediated by COX-2 and PGF2alpha. PMID: 27178425
  15. These data suggest that adaptive changes induced in the myocardium by CIH may include activation of cPLA2alpha and COX-2 via beta2-AR/Gi-mediated stimulation of the ERK/p38 pathway. PMID: 27686454
  16. COX-2 protein expression was upregulated in lung tissue in response to skeletal muscle ischemia reperfusion PMID: 26724476
  17. It was concluded that intestinal damage during acute pancreatitis is exacerbated in elderly rats compared with young rats and that COX-2 inhibition could be a potential therapeutic target to offer tailored treatment for acute pancreatitis in the elderly. PMID: 26610611
  18. TNFalpha-RelB/p52 pathway may be involved in the early stages of renal damage, in part by stimulating COX-2 and inflammatory responses PMID: 26824492
  19. COX1/2 inhibition alters the host response and reduces ECM scaffold mediated constructive tissue remodeling in a rodent model of skeletal muscle injury PMID: 26612417
  20. Low-salt diet modulates mesenteric vascular responses via increased NO bioavailability suggested by increased iNOS protein expression and vasoconstrictor prostanoid production via COX-2. PMID: 26685759
  21. These results suggest the existence of a causal link between Cox-2 and p53, which may represent a toxic mechanism of electrophilic lipid peroxidation products. PMID: 25506925
  22. Src modulates the 4-Hydroxynonenal-enhanced activation of AP-1 and the expression of COX-2 PMID: 26466383
  23. IL-1beta reduces tonic contraction of mesenteric lymphatic muscle cells, with the involvement of COX-2 and prostaglandin E2 PMID: 25989136
  24. Data show that mesenchymal stem cells (MSCs) administration alleviated airway inflammation and emphysema through the down-regulation of cyclooxygenase-2 (COX-2) via p38 and ERK MAPK pathways. PMID: 25736434
  25. increased COX-2-dependent vasoconstriction contributes to renal endothelial dysfunction through enhanced reactive oxygen species generation in obesity. PMID: 25841778
  26. The aim of this study was to determine the role of COX-2 in the regulation of blood pressure and to define the mechanisms in two kidney one-clip hypertensive (2K1C) rats PMID: 25846103
  27. Treatment with probiotics has the potential of providing protection against colon cancer by down-regulating the COX-2 expression as one of the protective mechanisms. PMID: 25811420
  28. TNF-alpha-induced COX-2 and PGE2 stimulate Wnt signaling and activate Wnt target genes PMID: 26123748
  29. Increased COX2 expression after l-DOPA long-term treatment in Parkinsonian-like rats could contribute to the development of dyskinesia. PMID: 26009769
  30. Concomitant use of alpha-lipoic acid and cyclosporine significantly increased nitric oxide production, cyclooxygenase-2 and miR-210 gene expression in rat colon. PMID: 26276312
  31. E2-BSA induced the COX-2 expression and consequent PGE2 and PGF2alpha release in rat OECs. These effects are mediated through GPR30-derived EGFR transactivation and PI-3K/Akt cascade leading to NF-kappaB activation PMID: 26241029
  32. Low-level laser therapy decreases Achilles tendon inflammatory process mediated by Il1b and COX-2. PMID: 25070591
  33. COX-2 oxidative metabolism is a key regulator of the anandamide-mediated decidual remodeling in pregnancy. PMID: 26335727
  34. Our results demonstrated that ventricular hypertrophy abrogated isoflurane-induced delayed cardioprotection by alteration of iNOS/COX-2 pathway PMID: 25400168
  35. Hypomethylation of PTGS2, and hypermethylation of APC2 may be causally linked to the etiology of oral cancer in a rat model. PMID: 25635769
  36. Clathrin-dependent internalization of the angiotensin II ATA receptor links receptor internalization to COX-2 protein expression in rat aortic vascular smooth muscle cells PMID: 25542758
  37. Report design of benzimidazole analogs endowed with oxadiazole as selective COX-2 inhibitors. PMID: 25303727
  38. Cadmum induced release of reactive oxygen species derived from NADPH oxidase may be due to the increased local release of angiotensin II and the release of vasoconstrictor prostanoids derived from the COX-2 pathway. PMID: 23973752
  39. Data suggest that cyclooxygenase-2 (COX2) and NADPHox are potential therapeutic targets to decrease testosterone-driven cardiovascular risk. PMID: 25700020
  40. data propose that an increase in COX-2 expression in ventral CA1 following trauma is likely due to its attenuated degradation. PMID: 25058273
  41. Data (including data from studies in transgenic rats) suggest that attenuation of circadian clock system, especially its core component Rev-erb-alpha, contributes to induction of Ptgs2 in endometrial stromal cells during decidualization/placentation. PMID: 25648833
  42. Nitric oxide synthase (NOS) and cyclooxygenase (COX) isoform expression patterns were determined in femoral artery of obese Zucker rats. PMID: 25216050
  43. Reflux of duodenal contents induces COX2 expression and increases prostaglandin synthesis in dysplastic and cancer tissues PMID: 24914375
  44. ischemia reperfusion injury significantly increased the COX2 expression, PGI2 and TXA2 levels, and the PGI2/TXA2 ratio in rat hippocampus in a time-dependent manner PMID: 25388440
  45. The present study showed that the group receiving Vitamin E supplementation at 3x recommended daily intake displayed reduced COX2 expression. PMID: 25123248
  46. during the early phase of ANG II-dependent hypertension, the increased (P)RR and COX-2 expression in the renal medulla may contribute to attenuate the vasoconstrictor effects of ANG II on renal hemodynamics PMID: 25143455
  47. The data suggest that local hypertonicity in the medullary thick ascending limb is associated with an increase in COX-2 expression concomitant with elevated EP3 receptor expression, which limits COX-2 activity in this segment of the nephron. PMID: 25080527
  48. Elevated COX-2 expression during hypoxia where angiogenesis occurs and re-oxygenation, when micro-vessels regress, identifies this protein as a vascular remodeling molecule crucial for angioplasticity. PMID: 24796880
  49. Fibroblasts activated by hepatoma cell supernatant express COX-2 and HGF at higher levels. PMID: 24815169
  50. Suggest lead-associated inflammatory neurotoxicity occurs via activation of pro-inflammatory NFAT3/COX-2 axis. PMID: 25193092

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Subcellular Location Microsome membrane, Peripheral membrane protein, Endoplasmic reticulum membrane, Peripheral membrane protein
Protein Families Prostaglandin G/H synthase family
Tissue Specificity Expressed throughout the forebrain in discrete populations of neurons and is enriched in the cortex and hippocampus.
Database Links

KEGG: rno:29527

STRING: 10116.ENSRNOP00000003567

UniGene: Rn.217585

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