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The rat COX-2 ELISA Kit quantitates rat COX-2 levels in serum, plasma, and tissue homogenates. COX-2 (PTGS2) is the key rate-limiting enzyme needed for the synthesis of prostanoids, including PGE2, PGD2, PGF2a, PGI2, and TAX2, from an essential fatty acid arachidonic acid (AA). It is constitutively expressed in certain cell groups of the brain and inducibly expressed in response to inflammatory reactions. In the central nervous system (CNS), COX-2 affects memory, sensory integration, and autonomic modulation. COX-2 overexpression is mainly linked to inflammation, apoptosis inhibition, uncontrolled cell proliferation, growth, metastasis, neovascularization, and angiogenesis finally resulting in cancer. COX-2 is often expressed in many malignant tumors and is associated with occurrence, progression, and prognosis.
This kit employs the sandwich-ELISA mechanism in conjugation with COX-2 antibody-COX-2 antigen-specific binding as well as HRP-TMB chromogenic reaction to measure the concentration of COX-2 in the samples. The kit is characterized by high sensitivity, strong specificity, good linearity, high recovery, and a precision of less than 10%.
|Target Name||prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase)|
Ptgs2 ELISA Kit; Cox-2 ELISA Kit; Cox2 ELISA Kit; Prostaglandin G/H synthase 2 ELISA Kit; EC 220.127.116.11 ELISA Kit; Cyclooxygenase-2 ELISA Kit; COX-2 ELISA Kit; PHS II ELISA Kit; Prostaglandin H2 synthase 2 ELISA Kit; PGH synthase 2 ELISA Kit; PGHS-2 ELISA Kit; Prostaglandin-endoperoxide synthase 2 ELISA Kit
|Species||Rattus norvegicus (Rat)|
|Sample Types||serum, plasma, tissue homogenates|
|Detection Range||1.56 ng/mL-100 ng/mL|
|Detection Wavelength||450 nm|
|ELISA Data Analysis||Watch ELISA data processing video & download Curve Expert if needed|
|ELISA kit FAQs|
|Storage||Store at 2-8°C. Please refer to protocol.|
|Lead Time||3-5 working days|
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How can I convert pyggrams per decilitre to picograms per milligram, because your kits are in deciliters and concentrates
this kits according to picogerm/dl, i want according to picogeram /mg
Dual cyclooxygenase and peroxidase in the biosynthesis pathway of prostanoids, a class of C20 oxylipins mainly derived from arachidonate, with a particular role in the inflammatory response. The cyclooxygenase activity oxygenates arachidonate (AA, C20:4(n-6)) to the hydroperoxy endoperoxide prostaglandin G2 (PGG2), and the peroxidase activity reduces PGG2 to the hydroxy endoperoxide PGH2, the precursor of all 2-series prostaglandins and thromboxanes. This complex transformation is initiated by abstraction of hydrogen at carbon 13 (with S-stereochemistry), followed by insertion of molecular O2 to form the endoperoxide bridge between carbon 9 and 11 that defines prostaglandins. The insertion of a second molecule of O2 (bis-oxygenase activity) yields a hydroperoxy group in PGG2 that is then reduced to PGH2 by two electrons. Similarly catalyzes successive cyclooxygenation and peroxidation of dihomo-gamma-linoleate (DGLA, C20:3(n-6)) and eicosapentaenoate (EPA, C20:5(n-3)) to corresponding PGH1 and PGH3, the precursors of 1- and 3-series prostaglandins. In an alternative pathway of prostanoid biosynthesis, converts 2-arachidonoyl lysophopholipids to prostanoid lysophopholipids, which are then hydrolyzed by intracellular phospholipases to release free prostanoids. Metabolizes 2-arachidonoyl glycerol yielding the glyceryl ester of PGH2, a process that can contribute to pain response. Generates lipid mediators from n-3 and n-6 polyunsaturated fatty acids (PUFAs) via a lipoxygenase-type mechanism. Oxygenates PUFAs to hydroperoxy compounds and then reduces them to corresponding alcohols. Plays a role in the generation of resolution phase interaction products (resolvins) during both sterile and infectious inflammation. Metabolizes docosahexaenoate (DHA, C22:6(n-3)) to 17R-HDHA, a precursor of the D-series resolvins (RvDs). As a component of the biosynthetic pathway of E-series resolvins (RvEs), converts eicosapentaenoate (EPA, C20:5(n-3)) primarily to 18S-HEPE that is further metabolized by ALOX5 and LTA4H to generate 18S-RvE1 and 18S-RvE2. In vascular endothelial cells, converts docosapentaenoate (DPA, C22:5(n-3)) to 13R-HDPA, a precursor for 13-series resolvins (RvTs) shown to activate macrophage phagocytosis during bacterial infection. In activated leukocytes, contributes to oxygenation of hydroxyeicosatetraenoates (HETE) to diHETES (5,15-diHETE and 5,11-diHETE). During neuroinflammation, plays a role in neuronal secretion of specialized preresolving mediators (SPMs) 15R-lipoxin A4 that regulates phagocytic microglia.
|Gene References into Functions||
|Subcellular Location||Microsome membrane; Peripheral membrane protein. Endoplasmic reticulum membrane; Peripheral membrane protein. Nucleus inner membrane; Peripheral membrane protein. Nucleus outer membrane; Peripheral membrane protein.|
|Protein Families||Prostaglandin G/H synthase family|
|Tissue Specificity||Expressed throughout the forebrain in discrete populations of neurons and is enriched in the cortex and hippocampus.|