Rat peroxisome proliferators activator receptors alpha,PPAR-α ELISA Kit

Instructions
Code CSB-E09755r
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name peroxisome proliferator-activated receptor alpha
Alternative Names Ppara ELISA Kit; Nr1c1 ELISA Kit; Ppar ELISA Kit; Peroxisome proliferator-activated receptor alpha ELISA Kit; PPAR-alpha ELISA Kit; Nuclear receptor subfamily 1 group C member 1 ELISA Kit
Abbreviation PPARA
Uniprot No. P37230
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 0.312 ng/mL-20 ng/mL
Sensitivity 0.078 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Metabolism
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat PPAR-α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 96  
Range % 89-100  
1:2 Average % 94  
Range % 88-98  
1:4 Average % 96  
Range % 91-100  
1:8 Average % 95  
Range % 87-101  
Recovery
The recovery of rat PPAR-α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 92 86-96  
EDTA plasma (n=4) 90 85-96  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected  
20 2.183 2.177 2.180 2.068  
10 1.654 1.627 1.641 1.529  
5 1.240 1.211 1.226 1.114  
2.5 0.859 0.842 0.851 0.739  
1.25 0.546 0.513 0.530 0.418  
0.625 0.381 0.373 0.377 0.265  
0.312 0.166 0.157 0.162 0.050  
0 0.111 0.113 0.112    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Background

Function
(From Uniprot)
Ligand-activated transcription factor. Key regulator of lipid metabolism. Activated by the endogenous ligand 1-palmitoyl-2-oleoyl-sn-glycerol-3-phosphocholine (16
Gene References into Functions
  1. MicroRNA-21 regulates peroxisome proliferator-activated receptor alpha expression in cardiorenal syndrome type 4, to alter cardiac pathology. PMID: 28760335
  2. The results suggested that PPARalpha activation may exert a protective effect against ischemia/reperfusion in the myocardium, at least in part via endoplasmic reticulum stress inhibition. PMID: 29568903
  3. Repeated activation of PPARalpha induced a decreased phosphorylation of beta2*nAChRs that was accompanied by a switch in firing pattern of spontaneously active dopamine cells. PPARalpha activation enhanced dopamine D1 receptor signaling in the nucleus accumbens shell (NAcS). Long-term PPARalpha stimulation restored dopamine signaling to sucrose consumption in the NAcS and appetitive motivation. PMID: 27457507
  4. findings unravel the mechanism of Arjunolic acid action in regressing hypertrophy-associated cardiac fibrosis by assigning a role of Arjunolic acid as a PPARalpha agonist that inactivates non-canonical TGF-beta signaling. PMID: 28821620
  5. Roux-en-Y gastric bypass upregulates striatal dopamine 1 receptor (D1R) expression specifically under high-fat diet feeding conditions. PMID: 28065827
  6. Metabolism of methiocarb and carbaryl alters PPAR alpha agonist activity. PMID: 27665777
  7. Ultraviolet absorber, 2-(2'-hydroxy-3',5'-di-tert-butylphenyl)benzotriazole exerts its hepatotoxicity through the PPARalpha signal pathway and the sex-related difference in PPARalpha expression may contribute to the sex-related difference in susceptibility to hepatotoxicity. PMID: 27665778
  8. Suggest the therapeutic effects of berberine on angiotensin IV-induced proliferation in cultured vascular smooth muscle cells are partially partially mediated by the PPARalpha-NO signalling pathway. PMID: 27927051
  9. PPARalpha-stimulation prevents overexpression of pro-inflammatory molecules in a model of acute myocardial infarction. PMID: 27050838
  10. UA inhibited the exaggerated spinal cord inflammatory response to peripheral inflammatory stimulation in HFDinduced obesity by restoring downregulated spinal PPARalpha, preventing peripheral inflammation and inflammatory hyperalgesia. PMID: 27108888
  11. PPAR-alpha-FGF21 induction by docosahexaenoic acid (DHA) and thyroid hormone (T3) may involve ligand activation of PPAR-alpha by DHA and enhanced expression of PPAR-alpha by T3. PMID: 27248050
  12. the results confirm the relation between the development of insulin-resistance with the over-expression of SREBP-1c, the reduction of PPAR-alpha expression and the predominance of large and triglyceride over-enriched VLDL circulating particles. PMID: 25796423
  13. GW0742 has the ability to improve glucose homeostasis in diabetic rats through activation of PPAR-delta. PMID: 26508837
  14. Stimulation of PPAR-alpha by medium chain triglycerides promotes reverse cardiac remodeling in chronic pressure overload. PMID: 25976666
  15. Age-related sensitivity to endotoxin-induced liver inflammation is mediated by IL-1beta-induced lipid accumulation in hepatocytes through the dysregulation of PPARa and SREBP1c. PMID: 25847140
  16. Fructose feeding significantly decreased mRNA levels for PPAR alpha and CPT1A. PMID: 26519879
  17. Data suggest that expression of PPARA in liver can be regulated by dietary factors; here, dietary supplementation with 5-caffeoylquinic acid up-regulates expression of PPARA and prevents high-fat diet induced obesity. PMID: 25186103
  18. Stimulation of PPARalpha by clofibrate prevents an increase in the activity of renin-angiotensin system and promotes the production of vasodilator substances in myocardial ischemia. PMID: 25658458
  19. Tau supplementation prevented obesity and hepatic TG deposition by upregulating MTP mRNA, ameliorating hepatic lipid efflux, and consequently enhancing PPAR-alpha which increases lipid oxidation through ACO and CPT-1a gene expressions. PMID: 26092479
  20. Findings suggest that diet-induced obesity causes down-regulation of spinal PPARalpha, which facilitates the susceptibility to peripheral inflammatory challenge by increasing inflammatory response in the spinal cord. PMID: 25135355
  21. results demonstrate that inhibition of PPARalpha attenuates the beneficial effects of metformin on mitochondria in acute IR. PMID: 22837722
  22. In cardiomyocytes the protective effects of metformin-induced AMPK activation against oxidative stress converge on mitochondria and are mediated, at least in part, through the dissociation of PPARalpha-CypD interactions. PMID: 25617357
  23. the phosphorylation of ERK1/2 inhibited the expression and activity of SCAD through the PPARalpha signaling pathway, which induced the development of pathological cardiomyocyte hypertrophy. PMID: 25636810
  24. PPARalpha contributes to regulation of blood pressure and vascular reactivity in SHR, and clofibrate-mediated reduction in blood pressure and proteinuria is probably through increased NO production. PMID: 24562305
  25. data uncover HILPDA as a novel PPAR target that raises hepatic triglyceride storage via regulation of triglyceride secretion. PMID: 24876382
  26. Peroxisomal multifunctional enzyme type 1 (rpMFE-1) undergoes Posttranslational Modification on several N epsilon-Lys residues under PPAR-alpha-mediated peroxisome proliferation. [peroxisomal multifunctional enzyme type 1] PMID: 24092543
  27. Systemic disorganization of functional activity of nuclear PPAR plays a key role in the molecular mechanisms of metabolic and immune disturbances in rats with inherited stress-induced arterial hypertension. PMID: 24288751
  28. These findings unveil novel roles for PPARalpha in mediating stress signals between hepatic subcellular stress-responding machinery PMID: 24735884
  29. After spinal cord injury, there is a long-lasting in the level of PPARalpha in white matter in the vicinity of the lesion site. PMID: 23958344
  30. Urotensin-II can protect renal tubular cells from apoptosis through PGI2-mediated PPARA and Akt activation. PMID: 23933501
  31. A decrease in the expression of Nrf2 and PPARalpha, together with an increase in NF-kappaB expression, was observed in the renal cortex of L-NAME-induced hypertensive rats compared with control rats. PMID: 23223967
  32. PPAR signaling pathway is activated following ethyl tertiary-butyl ether administration which leads to tumorigenesis. PMID: 24090815
  33. glomerular hypertrophic responses in rats on a HFD are associated with reduced PPARdelta expression and increased p38 MAPK phosphorylation. PMID: 23592661
  34. Growing evidence points to PPARalpha as an additional detector of metabolic signals and altered functional demands, able to set into motion prompt responses to cope with ongoing metabolic needs and maintain cell homeostasis. PMID: 23394525
  35. in ischemia-reperfusion, functional interplay between the decrease in L-carnitine and the PPARalpha/PGC1alpha pathway-induced redirection of fatty acid metabolism protects mitochondria against long chain fatty acid overload PMID: 23525500
  36. novel differences in the regulation of lipins 1,2,3, PPARalpha, and PGC-1alpha splice variants during fasting in heart versus liver, even though the ultimate outcome in both tissues is to increase FA turnover and oxidation. PMID: 23505321
  37. The hypocholesterolemic functions of chlorogenic acid are probably due to the increase in fatty acids unitization in liver via the up-regulation of peroxisome proliferation-activated receptor alpha mRNA. PMID: 22674675
  38. Activation of PPAR-alpha played a protective role in acute pancreatitis, partially mediated by modulation of TLR pathways. PMID: 22722259
  39. PPARalpha plays a role in the regulation of the ubiquitin proteasome system. PMID: 23041501
  40. PPARalpha significantly contributes to the effects of endogenous cholinergic transmission mediated by ss2-nicotinic acetylcholine receptors on dopamine (DA) neuron excitability and to the effects of nicotine on locomotor activity, a DA associated behavior. PMID: 20570248
  41. PPARalpha activation improves endothelial dysfunction and reduces fibrosis and portal pressure in cirrhotic rats. PMID: 22245887
  42. Pharmacological activation of PPARalpha improves metabolic milieu, steatosis, ballooning, and combats NF-kappaB and JNK activation, neutrophil and F4/80 macrophage recruitment in diabetes-related NASH. PMID: 21929649
  43. Peroxisome proliferator-activated receptor-gamma regulates inflammation and renin-angiotensin system activity in the hypothalamic paraventricular nucleus and ameliorates peripheral manifestations of heart failure. PMID: 22083161
  44. PPARalpha can compete with GATA-4 binding to NFATc4 PMID: 22198280
  45. Abnormal expressions of FXR and PPARa may play a role in pathogenesis of estrogen-induced intrahepatic cholestasis. PMID: 21205480
  46. findings suggest that increased PPAR-alpha expression in OLETF rats liver is a contributory factor to the exercise-related improvements in whole-body metabolism. PMID: 21618160
  47. Integrated investigation of PPAR, LXR, PXR, CAR regulatory mechanisms, signal transduction and transcriptional targets will provide insights into the pathogenesis of MS and offer valuable information for designing of drugs for MS treatment. PMID: 22066269
  48. Evidence indicating the involvement of the saturated acylethanolamide palmitoylethanolamide in allopregnanolone synthesis through PPAR-alpha in astrocytes. PMID: 21554431
  49. Activation of PPARalpha protects cells in dentate gyrus and hippocampus from excitotoxic insult. PMID: 20221904
  50. Upregulation of apoA5 expression contributes to the triglyceridelowering effect of statins via the PPARalpha signaling pathway. PMID: 21092650

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Subcellular Location Nucleus
Protein Families Nuclear hormone receptor family, NR1 subfamily
Tissue Specificity Expressed predominantly in liver and kidney.
Database Links

KEGG: rno:25747

STRING: 10116.ENSRNOP00000038651

UniGene: Rn.9753

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