Rat procollagen type III (HPCⅢ) ELISA Kit

Code CSB-E15810r
Size 96T,5×96T,10×96T
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now
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Product Details

Target Name
procollagen type III (HPCⅢ)
Alternative Names
procollagen type 3 (HPC3),procollagen type III (HPCIII)
Rattus norvegicus (Rat)
Sample Types
serum, plasma, cell culture supernates, tissue homogenates
Detection Range
0.156 ng/mL-10 ng/mL
0.039 ng/mL
Assay Time
Sample Volume
Detection Wavelength
450 nm
Research Area
Signal Transduction
Assay Principle
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
To assess the linearity of the assay, samples were spiked with high concentrations of rat HPCⅢ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:5 Average % 84  
Range % 80-88  
1:10 Average % 97  
Range % 91-102  
1:20 Average % 97  
Range % 92-104  
1:40 Average % 93  
Range % 90-96  
The recovery of rat HPCⅢ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 95 90-98  
EDTA plasma (n=4) 97 92-104  
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected  
10 2.589 2.636 2.613 2.426  
5 1.901 1.967 1.934 1.747  
2.5 1.455 1.468 1.462 1.275  
1.25 1.012 1.089 1.051 0.864  
0.625 0.587 0.597 0.592 0.405  
0.312 0.342 0.368 0.355 0.168  
0.156 0.282 0.289 0.286 0.099  
0 0.188 0.186 0.187    
and FAQs
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx

This Rat HPCⅢ ELISA Kit was designed for the quantitative measurement of Rat HPCⅢ protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.156 ng/mL-10 ng/mL and the sensitivity is 0.039 ng/mL.

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