Rat selenoprotein P, plasma, 1 (SEPP1)ELISA kit

Instructions
Code CSB-E17683r
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name selenoprotein P, plasma, 1 (SEPP1)ELISA kit
Alternative Names Selenop ELISA Kit; Selp ELISA Kit; Sepp1 ELISA Kit; Selenoprotein P ELISA Kit; SeP) [Cleaved into: Selenoprotein Se-P10; Selenoprotein Se-P6; Selenoprotein Se-P2; Selenoprotein Se-P1] ELISA Kit
Abbreviation SEPP1
Uniprot No. P25236
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, tissue homogenates
Detection Range 78 pg/mL-5000 pg/mL
Sensitivity 19.5 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Others
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat SEPP1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1000 Average % 92
Range % 88-96
1:2000 Average % 105
Range % 100-110
1:4000 Average % 96
Range % 92-100
1:8000 Average % 89
Range % 84-93
Recovery
The recovery of rat SEPP1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 98 94-102
EDTA plasma (n=4) 85 80-90
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
5000 2.522 2.496 2.509 2.354
2500 1.949 2.023 1.986 1.831
1250 1.613 1.584 1.599 1.444
625 1.186 1.135 1.161 1.006
312 0.754 0.721 0.738 0.583
156 0.448 0.460 0.454 0.299
78 0.315 0.297 0.306 0.151
0 0.151 0.158 0.155  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Data

Function Might be responsible for some of the extracellular antioxidant defense properties of selenium or might be involved in the transport of selenium. May supply selenium to tissues such as brain and testis.
Gene References into Functions
  1. A chromatin immunoprecipitation (ChIP) assay revealed that EPA decreases binding of SREBP-1c to the SELENOP promoter. Knockdown of Srebf1 resulted in a significant down-regulation of Selenop expression. PMID: 28465347
  2. Data indicate that Selenoprotein P SEPP1 processivity is intrinsic, but efficiency is governed by selenium levels. PMID: 25063811
  3. metformin decreases binding of FoxO3a, a direct target of AMPK, to the SEPP1 promoter. PMID: 24257750
  4. Sepp1 secreted from epithelial cells may support the intestinal immune system by providing immune cells (including plasma cells) with selenium PMID: 24157689
  5. apoER2-mediated uptake of long isoform Sepp1 is responsible for selenium distribution to tissues throughout the body. PMID: 22761431
  6. investigation of Sepp1 expression in adipocytes during adipogenesis and oxidative stress in models of obesity and insulin resistance PMID: 20959537
  7. tear SeP is a key molecule to protect the ocular surface cells against environmental oxidative stress PMID: 20360971
  8. mass spectrometric characterization of full-length protein and three isoforms shortened at the C terminus PMID: 11821412
  9. selenenylsulfide linkages Sec304-Cys314 and Cys306-Sec316 were present; selenoprotein P contains these two selenenylsulfide bonds PMID: 15712351
  10. Sertoli cell ApoER2 is a Sepp1 receptor and a component of the selenium delivery pathway to spermatogenic cells PMID: 17314095
  11. In conclusion, the selenoprotein P promoter is a target of the Akt/FoxO signal transduction cascade and SeP expression is regulated at the level of transcription by the forkhead box protein FoxO1a in human and rat hepatoma cells. PMID: 17986386

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Subcellular Location Secreted
Protein Families Selenoprotein P family
Tissue Specificity Widely expressed, mainly by the liver. Secreted in plasma.
Database Links

KEGG: rno:29360

UniGene: Rn.1451

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