Rat selenoprotein P, plasma, 1 (SEPP1)ELISA kit

Code CSB-E17683r
Size 96T,5×96T,10×96T
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Product Details

Target Name
selenoprotein P, plasma, 1 (SEPP1)ELISA kit
Alternative Names
Selenop ELISA Kit; Selp ELISA Kit; Sepp1 ELISA Kit; Selenoprotein P ELISA Kit; SeP) [Cleaved into: Selenoprotein Se-P10; Selenoprotein Se-P6; Selenoprotein Se-P2; Selenoprotein Se-P1] ELISA Kit
Abbreviation
SEPP1
Uniprot No.
Species
Rattus norvegicus (Rat)
Sample Types
serum, plasma, tissue homogenates
Detection Range
78 pg/mL-5000 pg/mL
Sensitivity
19.5 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Others
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat SEPP1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1000 Average % 92
Range % 88-96
1:2000 Average % 105
Range % 100-110
1:4000 Average % 96
Range % 92-100
1:8000 Average % 89
Range % 84-93
Recovery
The recovery of rat SEPP1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 98 94-102
EDTA plasma (n=4) 85 80-90
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
5000 2.522 2.496 2.509 2.354
2500 1.949 2.023 1.986 1.831
1250 1.613 1.584 1.599 1.444
625 1.186 1.135 1.161 1.006
312 0.754 0.721 0.738 0.583
156 0.448 0.460 0.454 0.299
78 0.315 0.297 0.306 0.151
0 0.151 0.158 0.155  
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Rat SEPP1 ELISA Kit was designed for the quantitative measurement of Rat SEPP1 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 78 pg/mL-5000 pg/mL and the sensitivity is 19.5 pg/mL.

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Target Background

Function
(From Uniprot)
Might be responsible for some of the extracellular antioxidant defense properties of selenium or might be involved in the transport of selenium. May supply selenium to tissues such as brain and testis.
Gene References into Functions
  1. A chromatin immunoprecipitation (ChIP) assay revealed that EPA decreases binding of SREBP-1c to the SELENOP promoter. Knockdown of Srebf1 resulted in a significant down-regulation of Selenop expression. PMID: 28465347
  2. Data indicate that Selenoprotein P SEPP1 processivity is intrinsic, but efficiency is governed by selenium levels. PMID: 25063811
  3. metformin decreases binding of FoxO3a, a direct target of AMPK, to the SEPP1 promoter. PMID: 24257750
  4. Sepp1 secreted from epithelial cells may support the intestinal immune system by providing immune cells (including plasma cells) with selenium PMID: 24157689
  5. apoER2-mediated uptake of long isoform Sepp1 is responsible for selenium distribution to tissues throughout the body. PMID: 22761431
  6. investigation of Sepp1 expression in adipocytes during adipogenesis and oxidative stress in models of obesity and insulin resistance PMID: 20959537
  7. tear SeP is a key molecule to protect the ocular surface cells against environmental oxidative stress PMID: 20360971
  8. mass spectrometric characterization of full-length protein and three isoforms shortened at the C terminus PMID: 11821412
  9. selenenylsulfide linkages Sec304-Cys314 and Cys306-Sec316 were present; selenoprotein P contains these two selenenylsulfide bonds PMID: 15712351
  10. Sertoli cell ApoER2 is a Sepp1 receptor and a component of the selenium delivery pathway to spermatogenic cells PMID: 17314095
  11. In conclusion, the selenoprotein P promoter is a target of the Akt/FoxO signal transduction cascade and SeP expression is regulated at the level of transcription by the forkhead box protein FoxO1a in human and rat hepatoma cells. PMID: 17986386

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Subcellular Location
Secreted.
Protein Families
Selenoprotein P family
Tissue Specificity
Widely expressed, mainly by the liver. Secreted in plasma.
Database Links

KEGG: rno:29360

UniGene: Rn.1451

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