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The Recombinant Human Mesothelin MSLN is expressed in mammalian cells and fused with a human IgG1 Fc tag at N-terminus. It contains amino acids of Glu296-Gly580 and has a theoretical molecular weight of 59.6kDa. The purity of this MSLN protein is greater than 90% as determined on the SDS-PAGE. Its endotoxin content is less than 1.0 EU/ug as determined by the LAL method. And it was verified to be an active protein in the function ELISA. Immobilized MSLN at 2 μg/ml can bind Anti-MSLN rabbit monoclonal antibody with the EC50 of 2.657-3.177 ng/ml. Immobilized MUC16 (CSB-MP704410HU3c7) at 10 μg/ml can bind MSLN wity the EC50 of 460.7-662.2 ng/ml.
Recombinant Human Mesothelin is valuable in cancer research as a target for therapy. It is also used for detecting anti-MLSN antibodies in functional ELISA.
Mesothelin is widely expressed in normal mesothelial cells, oncogenic mesothelial cells, and ovarian cancer cells. Faulty expression of MSLN plays a role in the multiplication and invasion of tumors. When it is overexpressed, mesothelin may induce MAPK, PI3K, and NF-kB pathways. The induction of these pathways plays a part in the metastasis, proliferation, and survival of cancerous cells. Mesothelin also interacts with CA125 to facilitate the implantation of ovarian cancer cells in the peritoneum and the migration of carcinogenic pancreas cells by altering cellular adhesion.
Applications : Antigen, Drug related studies
Review: I used CSB-MP015044HUc9 to perform an ELISA experiment with the anti-MSLN antibody, and the EC50 was 1.940-4.024ng/ml, showing good binding activity. Subsequently, it was used as an antigen for immunization, and the immune effect was very good. When used for ELISA screening, the antigen coating effect is also good, stable, and has good repeatability.