Code | CSB-MP015044HU |
MSDS | |
Size | $110 |
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To express the recombinant Human MSLN protein in mammalian cells, a DNA fragment encoding the Human MSLN protein (296-580aa) is inserted into a plasmid vector and transferred to the mammalian cells. Cells containing the plasmid are screened, cultured, and induced to express the MSLN protein. The protein carries a C-terminal hFc tag. Lysing the cells allows for the collection of the recombinant Human MSLN protein, which is purified through affinity purification and then identified through SDS-PAGE and subsequent staining of the gel with Coomassie Brilliant Blue. The purity of the recombinant Human MSLN protein obtained is greater than 85%.
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