Product Details

Purity

Greater than 95% as determined by SDS-PAGE.

Endotoxin

Less than 1.0 EU/ug as determined by LAL method.

Activity

Measured by its binding ability in a functional ELISA. Immobilized TNF-α (CSB-YP023955HU) at 5 μg/ml can bind human TNFR2, the EC₅₀ of human TNFR2 protein is 1.162-1.481 ng/ml.②Measured by its binding ability in a functional ELISA. Immobilized LTA (CSB-MP013218HU) at 5 μg/ml can bind human TNFRSF1B, the EC₅₀ is 1.632-2.699 ng/ml.

Target Names

TNFRSF1B

Uniprot No.

P20333

Research Area

Cancer

Alternative Names

(Tumor necrosis factor receptor 2) (TNF-R2) (Tumor necrosis factor receptor type II) (TNF-RII) (TNFR-II) (p75) (p80 TNF-alpha receptor) (CD120b) (Etanercept) (TBP-2) (TBPII)

Molecular Characterization

Species

Homo sapiens (Human)

Source

Mammalian cell

Expression Region

23-257aa

Target Protein Sequence

LPAQVAFTPYAPEPGSTCRLREYYDQTAQMCCSKCSPGQHAKVFCTKTSDTVCDSCEDSTYTQLWNWVPECLSCGSRCSSDQVETQACTREQNRICTCRPGWYCALSKQEGCRLCAPLRKCRPGFGVARPGTETSDVVCKPCAPGTFSNTTSSTDICRPHQICNVVAIPGNASMDAVCTSTSPTRSMAPGAVHLPQPVSTRSQHTQPTPEPSTAPSTSFLLPMGPSPPAEGSTGD

Mol. Weight

54.1 kDa

Protein Length

Partial

Tag Info

C-terminal hFc1-tagged

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

TNFRSF1B (TNF-R2/p75) mediates critical TNF-α signaling in immune regulation and tumor microenvironment modulation, making it a key target in oncology drug discovery. This recombinant human TNFR2 construct spans the extracellular ligand-binding domain (aa 23–257) with a C-terminal hFc1 tag, and its functional integrity is confirmed by ELISA binding to immobilized TNF-α with an EC50 of 1.162–1.481 ng/ml, supporting use in ligand-receptor interaction assays, competitive inhibition studies, and blocking antibody screening. Mammalian cell expression preserves native glycosylation and disulfide-dependent folding essential for conformationally accurate epitope mapping and affinity characterization by SPR or BLI. Purity exceeding 95% by SDS-PAGE combined with endotoxin levels below 1.0 EU/μg satisfies the criteria typical for therapeutic antibody validation and small-molecule inhibitor screening in cancer-focused programs.

Customer Reviews and Q&A

■ Customer Reviews

Average Rating:

4.0 - 1 reviews

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Applications : Antigen

Review: We validated the binding activity of human TNFRSF1B and ligand human TNFRSF1B through functional ELISA, and the results showed good binding activity, which is basically consistent with the data on the website. It was subsequently used as an antigen to immunize mouse, the titer was high, and ideal positive clones were screened through ELISA screening!

By Anonymous

Code	CSB-MP023978HU2
Abbreviation	Recombinant Human TNFRSF1B protein, partial (Active)
MSDS	MSDS Datasheet
Size	$116
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel. Activity Measured by its binding ability in a functional ELISA. Immobilized TNF-α (CSB-YP023955HU) at 5 μg/ml can bind human TNFR2, the EC₅₀ of human TNFR2 protein is 1.162-1.481 ng/ml. Biological Activity Assay Activity Measured by its binding ability in a functional ELISA. Immobilized LTA (CSB-MP013218HU) at 5 μg/ml can bind human TNFRSF1B, the EC₅₀ is 1.632-2.699 ng/ml. Biological Activity Assay
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Recombinant Human Tumor necrosis factor receptor superfamily member 1B (TNFRSF1B), partial (Active)

Product Details

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